Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
Pre-oxidation can be used against cyanobacteria at the water treatment plant intake to improve cell removal efficiency in down flow processes and reduce cyanotoxins concentrations. In this study, shotgun metagenomic sequencing was used to describe the functional capacity of a cyanobacterial bloom (a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Chemical Engineering Journal Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666821121000673 |
| _version_ | 1830283201834123264 |
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| author | Saber Moradinejad Hana Trigui Juan Francisco Guerra Maldonado B. Jesse Shapiro Yves Terrat Sébastien Sauvé Nathalie Fortin Arash Zamyadi Sarah Dorner Michèle Prévost |
| author_facet | Saber Moradinejad Hana Trigui Juan Francisco Guerra Maldonado B. Jesse Shapiro Yves Terrat Sébastien Sauvé Nathalie Fortin Arash Zamyadi Sarah Dorner Michèle Prévost |
| author_sort | Saber Moradinejad |
| collection | DOAJ |
| description | Pre-oxidation can be used against cyanobacteria at the water treatment plant intake to improve cell removal efficiency in down flow processes and reduce cyanotoxins concentrations. In this study, shotgun metagenomic sequencing was used to describe the functional capacity of a cyanobacterial bloom (at Lake Champlain, southern Quebec, Canada) before and after pre-oxidation using Cl2, KMnO4 and H2O2. The bloom samples were associated with two functional profile assemblages: that of August 1st (onset of the bloom) characterized by enrichment of genes related to nutrient uptake and that of August 13th-29th (towards the end of the sampling) associated with competition for resources and repair such as Photosynthesis, Protein metabolism and DNA metabolism. Different functional profile responses to oxidation with Cl2, KMnO4 and H2O2 was also identified as two-time points during the bloom (at the August 1st, and August 29th). On August 1st, chlorinated samples showed a progressive shift in functional profile: starting by acquiring and sequestering nutrient sources (e.g. Iron acquisition, carbohydrates) at low chlorine exposure (CT, concentration X contact time) level, followed by showing a stronger tendency toward dormancy and sporulation genes at high CT. Our results showed that following high CT of H2O2, the relative abundance of the cyanobacterial biomarkers decreased, regardless of the dominant cyanobacterial genus. The toxicity of the bloom before and after oxidation samples was assessed by droplet digital PCR (ddPCR) to measure the mcyD gene. Our results showed significant positive correlation between the mcyD gene copies number and microcystin concentrations in the bloom samples (before the oxidation). However, such correlation was not observed after oxidation. These results suggest that ddPCR can only be used to evaluate bloom toxicity before oxidation. |
| first_indexed | 2024-12-19T02:52:48Z |
| format | Article |
| id | doaj.art-a9942352854e4d3ab3503cd8a569b886 |
| institution | Directory Open Access Journal |
| issn | 2666-8211 |
| language | English |
| last_indexed | 2024-12-19T02:52:48Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chemical Engineering Journal Advances |
| spelling | doaj.art-a9942352854e4d3ab3503cd8a569b8862022-12-21T20:38:31ZengElsevierChemical Engineering Journal Advances2666-82112021-11-018100151Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidationSaber Moradinejad0Hana Trigui1Juan Francisco Guerra Maldonado2B. Jesse Shapiro3Yves Terrat4Sébastien Sauvé5Nathalie Fortin6Arash Zamyadi7Sarah Dorner8Michèle Prévost9NSERC Industrial Chair on Drinking Water, Department of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec H3T 1J4, Canada; Corresponding author.NSERC Industrial Chair on Drinking Water, Department of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec H3T 1J4, CanadaNSERC Industrial Chair on Drinking Water, Department of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec H3T 1J4, CanadaDepartment of biological science, Université de Montréal, Montréal, Québec, H2V 0B3, Canada; Department of Microbiology and Immunology, McGill University, Canada; McGill Genome Centre, McGill University, CanadaDepartment of biological science, Université de Montréal, Montréal, Québec, H2V 0B3, CanadaDépartement de Chimie, Université de Montréal, Montréal, QC, CanadaNational Research Council Canada, Energy, Mining and Environment, 6100 Royalmount Avenue, Montreal, QC H4P 2R2, CanadaWater Research Australia (WaterRA) Melbourne based position hosted by Melbourne Water, 990 La Trobe St, Docklands VIC 3008, Australia; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research (WEHI), 1G, Royal Parade, Parkville VIC 3052, AustraliaNSERC Industrial Chair on Drinking Water, Department of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec H3T 1J4, CanadaNSERC Industrial Chair on Drinking Water, Department of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec H3T 1J4, CanadaPre-oxidation can be used against cyanobacteria at the water treatment plant intake to improve cell removal efficiency in down flow processes and reduce cyanotoxins concentrations. In this study, shotgun metagenomic sequencing was used to describe the functional capacity of a cyanobacterial bloom (at Lake Champlain, southern Quebec, Canada) before and after pre-oxidation using Cl2, KMnO4 and H2O2. The bloom samples were associated with two functional profile assemblages: that of August 1st (onset of the bloom) characterized by enrichment of genes related to nutrient uptake and that of August 13th-29th (towards the end of the sampling) associated with competition for resources and repair such as Photosynthesis, Protein metabolism and DNA metabolism. Different functional profile responses to oxidation with Cl2, KMnO4 and H2O2 was also identified as two-time points during the bloom (at the August 1st, and August 29th). On August 1st, chlorinated samples showed a progressive shift in functional profile: starting by acquiring and sequestering nutrient sources (e.g. Iron acquisition, carbohydrates) at low chlorine exposure (CT, concentration X contact time) level, followed by showing a stronger tendency toward dormancy and sporulation genes at high CT. Our results showed that following high CT of H2O2, the relative abundance of the cyanobacterial biomarkers decreased, regardless of the dominant cyanobacterial genus. The toxicity of the bloom before and after oxidation samples was assessed by droplet digital PCR (ddPCR) to measure the mcyD gene. Our results showed significant positive correlation between the mcyD gene copies number and microcystin concentrations in the bloom samples (before the oxidation). However, such correlation was not observed after oxidation. These results suggest that ddPCR can only be used to evaluate bloom toxicity before oxidation.http://www.sciencedirect.com/science/article/pii/S2666821121000673CyanobacteriaOxidationFunctionBiomarkerStress |
| spellingShingle | Saber Moradinejad Hana Trigui Juan Francisco Guerra Maldonado B. Jesse Shapiro Yves Terrat Sébastien Sauvé Nathalie Fortin Arash Zamyadi Sarah Dorner Michèle Prévost Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation Chemical Engineering Journal Advances Cyanobacteria Oxidation Function Biomarker Stress |
| title | Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation |
| title_full | Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation |
| title_fullStr | Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation |
| title_full_unstemmed | Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation |
| title_short | Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation |
| title_sort | metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation |
| topic | Cyanobacteria Oxidation Function Biomarker Stress |
| url | http://www.sciencedirect.com/science/article/pii/S2666821121000673 |
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