Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil
Tandem repeat proteins (TRPs) are major immunoreactive proteins of <i>Ehrlichia canis</i>, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different <i>E. canis</i> isolates expressed on both reticulate a...
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MDPI AG
2020-10-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/7/4/165 |
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| author | Isis Indaiara Gonçalves Granjeiro Taques Amanda Noeli Silva Campos Mayara Lima Kavasaki Sayanne Luns Hatum de Almeida Daniel Moura de Aguiar |
| author_facet | Isis Indaiara Gonçalves Granjeiro Taques Amanda Noeli Silva Campos Mayara Lima Kavasaki Sayanne Luns Hatum de Almeida Daniel Moura de Aguiar |
| author_sort | Isis Indaiara Gonçalves Granjeiro Taques |
| collection | DOAJ |
| description | Tandem repeat proteins (TRPs) are major immunoreactive proteins of <i>Ehrlichia canis</i>, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different <i>E. canis</i> isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of <i>E. canis</i> in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 34% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (<i>n</i> = 5; 0.61%) reactive to the <i>E. canis</i> TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of <i>E. canis</i> are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil. |
| first_indexed | 2024-03-10T15:14:08Z |
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| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T15:14:08Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj.art-a99929416ad44c0ca0910026f34559f52023-11-20T19:05:35ZengMDPI AGVeterinary Sciences2306-73812020-10-017416510.3390/vetsci7040165Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in BrazilIsis Indaiara Gonçalves Granjeiro Taques0Amanda Noeli Silva Campos1Mayara Lima Kavasaki2Sayanne Luns Hatum de Almeida3Daniel Moura de Aguiar4Laboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilLaboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilLaboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilLaboratory of Parasitology and Parasitic Diseases, Veterinary Hospital, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilLaboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilTandem repeat proteins (TRPs) are major immunoreactive proteins of <i>Ehrlichia canis</i>, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different <i>E. canis</i> isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of <i>E. canis</i> in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 34% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (<i>n</i> = 5; 0.61%) reactive to the <i>E. canis</i> TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of <i>E. canis</i> are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil.https://www.mdpi.com/2306-7381/7/4/165dogsehrlichiosisELISAtick-borneTRP36TRP19 |
| spellingShingle | Isis Indaiara Gonçalves Granjeiro Taques Amanda Noeli Silva Campos Mayara Lima Kavasaki Sayanne Luns Hatum de Almeida Daniel Moura de Aguiar Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil Veterinary Sciences dogs ehrlichiosis ELISA tick-borne TRP36 TRP19 |
| title | Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil |
| title_full | Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil |
| title_fullStr | Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil |
| title_full_unstemmed | Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil |
| title_short | Geographic Distribution of <i>Ehrlichia canis</i> TRP Genotypes in Brazil |
| title_sort | geographic distribution of i ehrlichia canis i trp genotypes in brazil |
| topic | dogs ehrlichiosis ELISA tick-borne TRP36 TRP19 |
| url | https://www.mdpi.com/2306-7381/7/4/165 |
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