Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection.
IL-27 is an immunomodulatory cytokine with potent anti-HIV properties in PBMCs, CD4+ T cells, macrophages and immature dendritic cells. Previous smaller studies have suggested that HIV-1 infection may alter IL-27 and influence HIV-1 pathogenesis. The aim of this study was to examine the relationship...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4045808?pdf=render |
| _version_ | 1818151208924217344 |
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| author | Sanjay Swaminathan Zonghui Hu Adam W Rupert Jeanette M Higgins Robin L Dewar Randy Stevens Qian Chen Catherine A Rehm Julia A Metcalf Michael W Baseler H Clifford Lane Tomozumi Imamichi |
| author_facet | Sanjay Swaminathan Zonghui Hu Adam W Rupert Jeanette M Higgins Robin L Dewar Randy Stevens Qian Chen Catherine A Rehm Julia A Metcalf Michael W Baseler H Clifford Lane Tomozumi Imamichi |
| author_sort | Sanjay Swaminathan |
| collection | DOAJ |
| description | IL-27 is an immunomodulatory cytokine with potent anti-HIV properties in PBMCs, CD4+ T cells, macrophages and immature dendritic cells. Previous smaller studies have suggested that HIV-1 infection may alter IL-27 and influence HIV-1 pathogenesis. The aim of this study was to examine the relationship between plasma IL-27 levels in a well-characterised cohort of HIV-1 infected patients.Patients were stratified into four groups based on HIV-1 viral load and matched according to age, gender and those receiving antiretroviral treatment. IL-27 levels and C-reactive protein (CRP) were measured using electrochemiluminescence assays. D-dimer and CD4+ T cell counts were measured using an Enzyme Linked Fluorescence Assay and FACS, respectively. sCD14 and sCD163 were measured using ELISA. HIV-1 viral load was measured by bDNA or qRT-PCR assays.Plasma IL-27 levels were measured in 505 patients (462 HIV+, 43 controls). The mean level (±SEM) of IL-27 in controls was 2990.7±682.1 pg/ml, in the <50 copies/ml group it was 2008.0±274.8 pg/ml, in the 51-10,000 copies group it was 1468.7±172.3 pg/ml, in the 10,001-100,000 copies/ml group it was 1237.9±127.3 pg/ml and in the >100,000 copies/ml group it was 1590.1±223.7 pg/ml. No statistically significant difference in IL-27 levels between groups were seen. There were no correlations noted between IL-27 and HIV-1 viral load or CD4+ T cell counts. There was a small correlation noted between D-dimer and IL-27 (Spearman r = 0.09, p = 0.03) and sCD163 and IL-27 (Spearman r = 0.12, p = 0.005). No correlation was observed between IL-27 and CRP or sCD14 levels.This is the largest study examining the levels of plasma IL-27 in HIV-1 infection. While IL-27 levels are not significantly altered in HIV-1 infection compared to uninfected controls there may be a small association between IL-27 and D-dimer levels and IL-27 and sCD163 levels. |
| first_indexed | 2024-12-11T13:35:11Z |
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| id | doaj.art-a9a384aa9aaf4ffd8af6fd8476c47c44 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T13:35:11Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-a9a384aa9aaf4ffd8af6fd8476c47c442022-12-22T01:05:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9898910.1371/journal.pone.0098989Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection.Sanjay SwaminathanZonghui HuAdam W RupertJeanette M HigginsRobin L DewarRandy StevensQian ChenCatherine A RehmJulia A MetcalfMichael W BaselerH Clifford LaneTomozumi ImamichiIL-27 is an immunomodulatory cytokine with potent anti-HIV properties in PBMCs, CD4+ T cells, macrophages and immature dendritic cells. Previous smaller studies have suggested that HIV-1 infection may alter IL-27 and influence HIV-1 pathogenesis. The aim of this study was to examine the relationship between plasma IL-27 levels in a well-characterised cohort of HIV-1 infected patients.Patients were stratified into four groups based on HIV-1 viral load and matched according to age, gender and those receiving antiretroviral treatment. IL-27 levels and C-reactive protein (CRP) were measured using electrochemiluminescence assays. D-dimer and CD4+ T cell counts were measured using an Enzyme Linked Fluorescence Assay and FACS, respectively. sCD14 and sCD163 were measured using ELISA. HIV-1 viral load was measured by bDNA or qRT-PCR assays.Plasma IL-27 levels were measured in 505 patients (462 HIV+, 43 controls). The mean level (±SEM) of IL-27 in controls was 2990.7±682.1 pg/ml, in the <50 copies/ml group it was 2008.0±274.8 pg/ml, in the 51-10,000 copies group it was 1468.7±172.3 pg/ml, in the 10,001-100,000 copies/ml group it was 1237.9±127.3 pg/ml and in the >100,000 copies/ml group it was 1590.1±223.7 pg/ml. No statistically significant difference in IL-27 levels between groups were seen. There were no correlations noted between IL-27 and HIV-1 viral load or CD4+ T cell counts. There was a small correlation noted between D-dimer and IL-27 (Spearman r = 0.09, p = 0.03) and sCD163 and IL-27 (Spearman r = 0.12, p = 0.005). No correlation was observed between IL-27 and CRP or sCD14 levels.This is the largest study examining the levels of plasma IL-27 in HIV-1 infection. While IL-27 levels are not significantly altered in HIV-1 infection compared to uninfected controls there may be a small association between IL-27 and D-dimer levels and IL-27 and sCD163 levels.http://europepmc.org/articles/PMC4045808?pdf=render |
| spellingShingle | Sanjay Swaminathan Zonghui Hu Adam W Rupert Jeanette M Higgins Robin L Dewar Randy Stevens Qian Chen Catherine A Rehm Julia A Metcalf Michael W Baseler H Clifford Lane Tomozumi Imamichi Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection. PLoS ONE |
| title | Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection. |
| title_full | Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection. |
| title_fullStr | Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection. |
| title_full_unstemmed | Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection. |
| title_short | Plasma interleukin-27 (IL-27) levels are not modulated in patients with chronic HIV-1 infection. |
| title_sort | plasma interleukin 27 il 27 levels are not modulated in patients with chronic hiv 1 infection |
| url | http://europepmc.org/articles/PMC4045808?pdf=render |
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