Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis.
To explore the expression level of Nrf2 in adenomyosis and study the mechanism of abnormal expression of Nrf2 in the pathogenesis of adenomyosis.Western blot, immunohistochemistry(IHC) and real time PCR were used to measure Nrf2 expression levels in tissue and cell samples. Knockdown and overexpress...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5560740?pdf=render |
| _version_ | 1818581704962473984 |
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| author | Ning Chen Baoying Du Hao Zhou Fengxian Shen Juan Li Zhenwei Xie |
| author_facet | Ning Chen Baoying Du Hao Zhou Fengxian Shen Juan Li Zhenwei Xie |
| author_sort | Ning Chen |
| collection | DOAJ |
| description | To explore the expression level of Nrf2 in adenomyosis and study the mechanism of abnormal expression of Nrf2 in the pathogenesis of adenomyosis.Western blot, immunohistochemistry(IHC) and real time PCR were used to measure Nrf2 expression levels in tissue and cell samples. Knockdown and overexpression of Nrf2 were used to investigate the variation of migration ability of endometrial glandular cells as well as the regulatory mechanism.Nrf2 protein levels were significantly higher in the eutopic and ectopic endometrial glands when compared with control cases using IHC and western blot methods. (p< 0.05). However, there was no statistical difference in Nrf2 mRNA expression levels between the adenomyosis and control groups. Using an agonist and Nrf2 siRNA, we regulated the Nrf2 protein levels of primary cultured endometrial glandular cells. With increased expression of Nrf2, cell scratch assay showed that the agonist-treated group migrated significantly faster than the control group, with MMP9 protein level markedly elevated. In contrast, Nrf2 siRNA-treated group migrated slower than the control group, with decreased expression of MMP9 protein. All of the scratching healing spaces and protein levels between the treated and control groups were statistically significant (p< 0.05).Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. Specified reduction of Nrf2 expression could prove to be a new therapeutic target in the clinical treatment of adenomyosis. |
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| format | Article |
| id | doaj.art-a9a3f6642e564336a2ef33f477f9f090 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T07:37:44Z |
| publishDate | 2017-01-01 |
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| spelling | doaj.art-a9a3f6642e564336a2ef33f477f9f0902022-12-21T22:39:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018277310.1371/journal.pone.0182773Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis.Ning ChenBaoying DuHao ZhouFengxian ShenJuan LiZhenwei XieTo explore the expression level of Nrf2 in adenomyosis and study the mechanism of abnormal expression of Nrf2 in the pathogenesis of adenomyosis.Western blot, immunohistochemistry(IHC) and real time PCR were used to measure Nrf2 expression levels in tissue and cell samples. Knockdown and overexpression of Nrf2 were used to investigate the variation of migration ability of endometrial glandular cells as well as the regulatory mechanism.Nrf2 protein levels were significantly higher in the eutopic and ectopic endometrial glands when compared with control cases using IHC and western blot methods. (p< 0.05). However, there was no statistical difference in Nrf2 mRNA expression levels between the adenomyosis and control groups. Using an agonist and Nrf2 siRNA, we regulated the Nrf2 protein levels of primary cultured endometrial glandular cells. With increased expression of Nrf2, cell scratch assay showed that the agonist-treated group migrated significantly faster than the control group, with MMP9 protein level markedly elevated. In contrast, Nrf2 siRNA-treated group migrated slower than the control group, with decreased expression of MMP9 protein. All of the scratching healing spaces and protein levels between the treated and control groups were statistically significant (p< 0.05).Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. Specified reduction of Nrf2 expression could prove to be a new therapeutic target in the clinical treatment of adenomyosis.http://europepmc.org/articles/PMC5560740?pdf=render |
| spellingShingle | Ning Chen Baoying Du Hao Zhou Fengxian Shen Juan Li Zhenwei Xie Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. PLoS ONE |
| title | Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. |
| title_full | Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. |
| title_fullStr | Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. |
| title_full_unstemmed | Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. |
| title_short | Abnormal expression of Nrf2 may play an important role in the pathogenesis and development of adenomyosis. |
| title_sort | abnormal expression of nrf2 may play an important role in the pathogenesis and development of adenomyosis |
| url | http://europepmc.org/articles/PMC5560740?pdf=render |
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