Thioester‐Based Coupled Fluorogenic Assays in Microdevice for the Detection of Single‐Molecule Enzyme Activities of Esterases with Specified Substrate Recognition

Thioester‐Based Coupled Fluorogenic Assays in Microdevice for the Detection of Single‐Molecule Enzyme Activities of Esterases with Specified Substrate Recognition

Abstract Single‐molecule enzyme activity assay is a platform that enables the analysis of enzyme activities at single proteoform level. The limitation of the targetable enzymes is the major drawback of the assay, but the general assay platform is reported to study single‐molecule enzyme activities o...

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Bibliographic Details
Main Authors: Tatsuya Ukegawa, Toru Komatsu, Mayano Minoda, Takuya Matsumoto, Takumi Iwasaka, Tadahaya Mizuno, Ryo Tachibana, Shingo Sakamoto, Kenjiro Hanaoka, Hiroyuki Kusuhara, Kazufumi Honda, Rikiya Watanabe, Yasuteru Urano
Format: Article
Language:English
Published: Wiley 2024-03-01
Series:Advanced Science
Subjects:
chemical biology
enzymes
enzymomics
single‐molecule analysis
Online Access:https://doi.org/10.1002/advs.202306559

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