Self-assembly of proteins and their nucleic acids
<p>Abstract</p> <p>We have developed an artificial protein scaffold, herewith called a protein vector, which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2003-01-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://www.jnanobiotechnology.com/content/1/1/1 |
| _version_ | 1798038397663576064 |
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| author | Terrett Jon Mason Sean Fletcher Graham Soloviev Mikhail |
| author_facet | Terrett Jon Mason Sean Fletcher Graham Soloviev Mikhail |
| author_sort | Terrett Jon |
| collection | DOAJ |
| description | <p>Abstract</p> <p>We have developed an artificial protein scaffold, herewith called a protein vector, which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical linkage between a functional protein and its genetic code. The principle is demonstrated using a streptavidin-based protein vector (SAPV) as both a nucleic acid binding pocket and a protein display system. We have shown that functional proteins or protein domains can be produced in vitro and physically linked to their DNA in a single enzymatic reaction. Such self-assembled protein-DNA complexes can be used for protein cloning, the cloning of protein affinity reagents or for the production of proteins which self-assemble on a variety of solid supports. Self-assembly can be utilised for making libraries of protein-DNA complexes or for labelling the protein part of such a complex to a high specific activity by labelling the nucleic acid associated with the protein. In summary, self-assembly offers an opportunity to quickly generate cheap protein affinity reagents, which can also be efficiently labelled, for use in traditional affinity assays or for protein arrays instead of conventional antibodies.</p> |
| first_indexed | 2024-04-11T21:39:39Z |
| format | Article |
| id | doaj.art-a9a89de8ca774896b9c8d5372a5eb4ca |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T21:39:39Z |
| publishDate | 2003-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-a9a89de8ca774896b9c8d5372a5eb4ca2022-12-22T04:01:39ZengBMCJournal of Nanobiotechnology1477-31552003-01-0111110.1186/1477-3155-1-1Self-assembly of proteins and their nucleic acidsTerrett JonMason SeanFletcher GrahamSoloviev Mikhail<p>Abstract</p> <p>We have developed an artificial protein scaffold, herewith called a protein vector, which allows linking of an in-vitro synthesised protein to the nucleic acid which encodes it through the process of self-assembly. This protein vector enables the direct physical linkage between a functional protein and its genetic code. The principle is demonstrated using a streptavidin-based protein vector (SAPV) as both a nucleic acid binding pocket and a protein display system. We have shown that functional proteins or protein domains can be produced in vitro and physically linked to their DNA in a single enzymatic reaction. Such self-assembled protein-DNA complexes can be used for protein cloning, the cloning of protein affinity reagents or for the production of proteins which self-assemble on a variety of solid supports. Self-assembly can be utilised for making libraries of protein-DNA complexes or for labelling the protein part of such a complex to a high specific activity by labelling the nucleic acid associated with the protein. In summary, self-assembly offers an opportunity to quickly generate cheap protein affinity reagents, which can also be efficiently labelled, for use in traditional affinity assays or for protein arrays instead of conventional antibodies.</p>http://www.jnanobiotechnology.com/content/1/1/1self-assemblyproteinDNAmolecular engineeringmolecular interfacecloning expression |
| spellingShingle | Terrett Jon Mason Sean Fletcher Graham Soloviev Mikhail Self-assembly of proteins and their nucleic acids Journal of Nanobiotechnology self-assembly protein DNA molecular engineering molecular interface cloning expression |
| title | Self-assembly of proteins and their nucleic acids |
| title_full | Self-assembly of proteins and their nucleic acids |
| title_fullStr | Self-assembly of proteins and their nucleic acids |
| title_full_unstemmed | Self-assembly of proteins and their nucleic acids |
| title_short | Self-assembly of proteins and their nucleic acids |
| title_sort | self assembly of proteins and their nucleic acids |
| topic | self-assembly protein DNA molecular engineering molecular interface cloning expression |
| url | http://www.jnanobiotechnology.com/content/1/1/1 |
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