Modified polymeric biomaterials with antimicrobial and immunomodulating properties
Abstract The modification of the surgical polypropylene mesh and the polytetrafluoroethylene vascular prosthesis with cecropin A (small peptide) and puromycin (aminonucleoside) yielded very stable preparations of modified biomaterials. The main emphasis was placed on analyses of their antimicrobial...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-58730-3 |
| _version_ | 1797219887232319488 |
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| author | Katarzyna Szałapata Mateusz Pięt Martyna Kasela Marcin Grąz Justyna Kapral-Piotrowska Aleksandra Mordzińska-Rak Elżbieta Samorek Paulina Pieniądz Jolanta Polak Monika Osińska-Jaroszuk Roman Paduch Bożena Pawlikowska-Pawlęga Anna Malm Anna Jarosz-Wilkołazka |
| author_facet | Katarzyna Szałapata Mateusz Pięt Martyna Kasela Marcin Grąz Justyna Kapral-Piotrowska Aleksandra Mordzińska-Rak Elżbieta Samorek Paulina Pieniądz Jolanta Polak Monika Osińska-Jaroszuk Roman Paduch Bożena Pawlikowska-Pawlęga Anna Malm Anna Jarosz-Wilkołazka |
| author_sort | Katarzyna Szałapata |
| collection | DOAJ |
| description | Abstract The modification of the surgical polypropylene mesh and the polytetrafluoroethylene vascular prosthesis with cecropin A (small peptide) and puromycin (aminonucleoside) yielded very stable preparations of modified biomaterials. The main emphasis was placed on analyses of their antimicrobial activity and potential immunomodulatory and non-cytotoxic properties towards the CCD841 CoTr model cell line. Cecropin A did not significantly affect the viability or proliferation of the CCD 841 CoTr cells, regardless of its soluble or immobilized form. In contrast, puromycin did not induce a significant decrease in the cell viability or proliferation in the immobilized form but significantly decreased cell viability and proliferation when administered in the soluble form. The covalent immobilization of these two molecules on the surface of biomaterials resulted in stable preparations that were able to inhibit the multiplication of Staphylococcus aureus and S. epidermidis strains. It was also found that the preparations induced the production of cytokines involved in antibacterial protection mechanisms and stimulated the immune response. The key regulator of this activity may be related to TLR4, a receptor recognizing bacterial LPS. In the present study, these factors were produced not only in the conditions of LPS stimulation but also in the absence of LPS, which indicates that cecropin A- and puromycin-modified biomaterials may upregulate pathways leading to humoral antibacterial immune response. |
| first_indexed | 2024-04-24T12:40:47Z |
| format | Article |
| id | doaj.art-a9aec7ab2be549bd8a9d47d8afc43436 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:40:47Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a9aec7ab2be549bd8a9d47d8afc434362024-04-07T11:14:50ZengNature PortfolioScientific Reports2045-23222024-04-0114111710.1038/s41598-024-58730-3Modified polymeric biomaterials with antimicrobial and immunomodulating propertiesKatarzyna Szałapata0Mateusz Pięt1Martyna Kasela2Marcin Grąz3Justyna Kapral-Piotrowska4Aleksandra Mordzińska-Rak5Elżbieta Samorek6Paulina Pieniądz7Jolanta Polak8Monika Osińska-Jaroszuk9Roman Paduch10Bożena Pawlikowska-Pawlęga11Anna Malm12Anna Jarosz-Wilkołazka13Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Pharmaceutical Microbiology, Medical University of LublinDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityDepartment of Pharmaceutical Microbiology, Medical University of LublinDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska UniversityAbstract The modification of the surgical polypropylene mesh and the polytetrafluoroethylene vascular prosthesis with cecropin A (small peptide) and puromycin (aminonucleoside) yielded very stable preparations of modified biomaterials. The main emphasis was placed on analyses of their antimicrobial activity and potential immunomodulatory and non-cytotoxic properties towards the CCD841 CoTr model cell line. Cecropin A did not significantly affect the viability or proliferation of the CCD 841 CoTr cells, regardless of its soluble or immobilized form. In contrast, puromycin did not induce a significant decrease in the cell viability or proliferation in the immobilized form but significantly decreased cell viability and proliferation when administered in the soluble form. The covalent immobilization of these two molecules on the surface of biomaterials resulted in stable preparations that were able to inhibit the multiplication of Staphylococcus aureus and S. epidermidis strains. It was also found that the preparations induced the production of cytokines involved in antibacterial protection mechanisms and stimulated the immune response. The key regulator of this activity may be related to TLR4, a receptor recognizing bacterial LPS. In the present study, these factors were produced not only in the conditions of LPS stimulation but also in the absence of LPS, which indicates that cecropin A- and puromycin-modified biomaterials may upregulate pathways leading to humoral antibacterial immune response.https://doi.org/10.1038/s41598-024-58730-3 |
| spellingShingle | Katarzyna Szałapata Mateusz Pięt Martyna Kasela Marcin Grąz Justyna Kapral-Piotrowska Aleksandra Mordzińska-Rak Elżbieta Samorek Paulina Pieniądz Jolanta Polak Monika Osińska-Jaroszuk Roman Paduch Bożena Pawlikowska-Pawlęga Anna Malm Anna Jarosz-Wilkołazka Modified polymeric biomaterials with antimicrobial and immunomodulating properties Scientific Reports |
| title | Modified polymeric biomaterials with antimicrobial and immunomodulating properties |
| title_full | Modified polymeric biomaterials with antimicrobial and immunomodulating properties |
| title_fullStr | Modified polymeric biomaterials with antimicrobial and immunomodulating properties |
| title_full_unstemmed | Modified polymeric biomaterials with antimicrobial and immunomodulating properties |
| title_short | Modified polymeric biomaterials with antimicrobial and immunomodulating properties |
| title_sort | modified polymeric biomaterials with antimicrobial and immunomodulating properties |
| url | https://doi.org/10.1038/s41598-024-58730-3 |
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