Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ<sup>9</sup>-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Del...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/11/9486 |
| Summary: | Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ<sup>9</sup>-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ<sup>8</sup>-THC), an isomer of Δ<sup>9</sup>-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ<sup>8</sup>-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ<sup>8</sup>-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ<sup>8</sup>-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ<sup>8</sup>-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways. |
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| ISSN: | 1661-6596 1422-0067 |