Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ<sup>9</sup>-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Del...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/11/9486 |
| _version_ | 1797597425835180032 |
|---|---|
| author | Ivan Anchesi Giovanni Schepici Luigi Chiricosta Agnese Gugliandolo Stefano Salamone Diego Caprioglio Federica Pollastro Emanuela Mazzon |
| author_facet | Ivan Anchesi Giovanni Schepici Luigi Chiricosta Agnese Gugliandolo Stefano Salamone Diego Caprioglio Federica Pollastro Emanuela Mazzon |
| author_sort | Ivan Anchesi |
| collection | DOAJ |
| description | Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ<sup>9</sup>-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ<sup>8</sup>-THC), an isomer of Δ<sup>9</sup>-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ<sup>8</sup>-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ<sup>8</sup>-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ<sup>8</sup>-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ<sup>8</sup>-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways. |
| first_indexed | 2024-03-11T03:05:54Z |
| format | Article |
| id | doaj.art-a9b05e1b606f4addb90ba68e69fb3d49 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:05:54Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a9b05e1b606f4addb90ba68e69fb3d492023-11-18T07:59:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012411948610.3390/ijms24119486Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic StudyIvan Anchesi0Giovanni Schepici1Luigi Chiricosta2Agnese Gugliandolo3Stefano Salamone4Diego Caprioglio5Federica Pollastro6Emanuela Mazzon7IRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyCannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ<sup>9</sup>-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ<sup>8</sup>-THC), an isomer of Δ<sup>9</sup>-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ<sup>8</sup>-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ<sup>8</sup>-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ<sup>8</sup>-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ<sup>8</sup>-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways.https://www.mdpi.com/1422-0067/24/11/9486delta-8-tetrahydrocannabinolSH-SY5Y cellstranscriptomic analysisglutamatergic synapsescholinergic synapsesdopaminergic synapses |
| spellingShingle | Ivan Anchesi Giovanni Schepici Luigi Chiricosta Agnese Gugliandolo Stefano Salamone Diego Caprioglio Federica Pollastro Emanuela Mazzon Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study International Journal of Molecular Sciences delta-8-tetrahydrocannabinol SH-SY5Y cells transcriptomic analysis glutamatergic synapses cholinergic synapses dopaminergic synapses |
| title | Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
| title_full | Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
| title_fullStr | Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
| title_full_unstemmed | Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
| title_short | Δ<sup>8</sup>-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
| title_sort | δ sup 8 sup thc induces up regulation of glutamatergic pathway genes in differentiated sh sy5y a transcriptomic study |
| topic | delta-8-tetrahydrocannabinol SH-SY5Y cells transcriptomic analysis glutamatergic synapses cholinergic synapses dopaminergic synapses |
| url | https://www.mdpi.com/1422-0067/24/11/9486 |
| work_keys_str_mv | AT ivananchesi dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT giovannischepici dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT luigichiricosta dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT agnesegugliandolo dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT stefanosalamone dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT diegocaprioglio dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT federicapollastro dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy AT emanuelamazzon dsup8supthcinducesupregulationofglutamatergicpathwaygenesindifferentiatedshsy5yatranscriptomicstudy |