Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico
Background: Histoplasma capsulatum and Pneumocystis jirovecii are respiratory fungal pathogens that principally cause pulmonary disease. Coinfection with both pathogens is scarcely reported. This study detected this coinfection using specific molecular methods for each fungus in the bronchoalveolar...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-09-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971219302553 |
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| author | Laura E. Carreto-Binaghi Fernando R. Morales-Villarreal Guadalupe García-de la Torre Tania Vite-Garín Jose-Antonio Ramirez El-Moukhtar Aliouat Jose-Arturo Martínez-Orozco Maria-Lucia Taylor |
| author_facet | Laura E. Carreto-Binaghi Fernando R. Morales-Villarreal Guadalupe García-de la Torre Tania Vite-Garín Jose-Antonio Ramirez El-Moukhtar Aliouat Jose-Arturo Martínez-Orozco Maria-Lucia Taylor |
| author_sort | Laura E. Carreto-Binaghi |
| collection | DOAJ |
| description | Background: Histoplasma capsulatum and Pneumocystis jirovecii are respiratory fungal pathogens that principally cause pulmonary disease. Coinfection with both pathogens is scarcely reported. This study detected this coinfection using specific molecular methods for each fungus in the bronchoalveolar lavage (BAL) of patients from a tertiary care hospital. Materials and methods: BAL samples from 289 hospitalized patients were screened by PCR with specific markers for H. capsulatum (Hcp100) and P. jirovecii (mtLSUrRNA and mtSSUrRNA). The presence of these pathogens was confirmed by the generated sequences for each marker. The clinical and laboratory data for the patients were analyzed using statistical software. Results: The PCR findings separated three groups of patients, where the first was represented by 60 (20.8%) histoplasmosis patients, the second by 45 (15.6%) patients with pneumocystosis, and the last group by 12 (4.2%) patients with coinfection. High similarity among the generated sequences of each species was demonstrated by BLASTn and neighbor-joining algorithms. The estimated prevalence of H. capsulatum and P. jirovecii coinfection was higher in HIV patients. Keywords: Histoplasmosis, Pneumocystosis, Coinfection, Molecular diagnosis, Bronchoalveolar lavage |
| first_indexed | 2024-12-21T22:53:52Z |
| format | Article |
| id | doaj.art-a9b1506e7c714ff1a3c2415ab7da9955 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-21T22:53:52Z |
| publishDate | 2019-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-a9b1506e7c714ff1a3c2415ab7da99552022-12-21T18:47:29ZengElsevierInternational Journal of Infectious Diseases1201-97122019-09-01866572Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in MexicoLaura E. Carreto-Binaghi0Fernando R. Morales-Villarreal1Guadalupe García-de la Torre2Tania Vite-Garín3Jose-Antonio Ramirez4El-Moukhtar Aliouat5Jose-Arturo Martínez-Orozco6Maria-Lucia Taylor7Laboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CDMX, 04510, Mexico; Departamento de Investigación en Microbiología, Instituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas” (INER), CDMX, 14080, MexicoDepartamento de Microbiología Clínica, INER, CDMX, 14080, MexicoDepartamento de Salud Pública, Facultad de Medicina, UNAM, CDMX, 04510, MexicoLaboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CDMX, 04510, MexicoLaboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CDMX, 04510, MexicoUniv. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Centre d’Infection et d’Immunité de Lille, Lille, FranceDepartamento de Microbiología Clínica, INER, CDMX, 14080, MexicoLaboratorio de Inmunología de Hongos, Unidad de Micología, Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), CDMX, 04510, Mexico; Corresponding author.Background: Histoplasma capsulatum and Pneumocystis jirovecii are respiratory fungal pathogens that principally cause pulmonary disease. Coinfection with both pathogens is scarcely reported. This study detected this coinfection using specific molecular methods for each fungus in the bronchoalveolar lavage (BAL) of patients from a tertiary care hospital. Materials and methods: BAL samples from 289 hospitalized patients were screened by PCR with specific markers for H. capsulatum (Hcp100) and P. jirovecii (mtLSUrRNA and mtSSUrRNA). The presence of these pathogens was confirmed by the generated sequences for each marker. The clinical and laboratory data for the patients were analyzed using statistical software. Results: The PCR findings separated three groups of patients, where the first was represented by 60 (20.8%) histoplasmosis patients, the second by 45 (15.6%) patients with pneumocystosis, and the last group by 12 (4.2%) patients with coinfection. High similarity among the generated sequences of each species was demonstrated by BLASTn and neighbor-joining algorithms. The estimated prevalence of H. capsulatum and P. jirovecii coinfection was higher in HIV patients. Keywords: Histoplasmosis, Pneumocystosis, Coinfection, Molecular diagnosis, Bronchoalveolar lavagehttp://www.sciencedirect.com/science/article/pii/S1201971219302553 |
| spellingShingle | Laura E. Carreto-Binaghi Fernando R. Morales-Villarreal Guadalupe García-de la Torre Tania Vite-Garín Jose-Antonio Ramirez El-Moukhtar Aliouat Jose-Arturo Martínez-Orozco Maria-Lucia Taylor Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico International Journal of Infectious Diseases |
| title | Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico |
| title_full | Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico |
| title_fullStr | Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico |
| title_full_unstemmed | Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico |
| title_short | Histoplasma capsulatum and Pneumocystis jirovecii coinfection in hospitalized HIV and non-HIV patients from a tertiary care hospital in Mexico |
| title_sort | histoplasma capsulatum and pneumocystis jirovecii coinfection in hospitalized hiv and non hiv patients from a tertiary care hospital in mexico |
| url | http://www.sciencedirect.com/science/article/pii/S1201971219302553 |
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