Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma

Various environmental factors can alter the gut microbiome’s composition and functionality, and modulate host health. In this study, the effects of oral and parenteral administration of two poorly bioavailable antibiotics (i.e., vancomycin and streptomycin) on male Wistar Crl/Wi(Han) rats for 28 day...

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Main Authors: Véronique de Bruijn, Christina Behr, Saskia Sperber, Tilmann Walk, Philipp Ternes, Markus Slopianka, Volker Haake, Karsten Beekmann, Bennard van Ravenzwaay
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Metabolites
Subjects:
Online Access:https://www.mdpi.com/2218-1989/10/6/242
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author Véronique de Bruijn
Christina Behr
Saskia Sperber
Tilmann Walk
Philipp Ternes
Markus Slopianka
Volker Haake
Karsten Beekmann
Bennard van Ravenzwaay
author_facet Véronique de Bruijn
Christina Behr
Saskia Sperber
Tilmann Walk
Philipp Ternes
Markus Slopianka
Volker Haake
Karsten Beekmann
Bennard van Ravenzwaay
author_sort Véronique de Bruijn
collection DOAJ
description Various environmental factors can alter the gut microbiome’s composition and functionality, and modulate host health. In this study, the effects of oral and parenteral administration of two poorly bioavailable antibiotics (i.e., vancomycin and streptomycin) on male Wistar Crl/Wi(Han) rats for 28 days were compared to distinguish between microbiome-derived or -associated and systemic changes in the plasma metabolome. The resulting changes in the plasma metabolome were compared to the effects of a third reference compound, roxithromycin, which is readily bioavailable. A community analysis revealed that the oral administration of vancomycin and roxithromycin in particular leads to an altered microbial population. Antibiotic-induced changes depending on the administration routes were observed in plasma metabolite levels. Indole-3-acetic acid (IAA) and hippuric acid (HA) were identified as key metabolites of microbiome modulation, with HA being the most sensitive. Even though large variations in the plasma bile acid pool between and within rats were observed, the change in microbiome community was observed to alter the composition of the bile acid pool, especially by an accumulation of taurine-conjugated primary bile acids. In-depth investigation of the relationship between microbiome variability and their functionality, with emphasis on the bile acid pool, will be necessary to better assess the potential adverseness of environmentally induced microbiome changes.
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spelling doaj.art-a9b34ee77ea14fbaad96bf6d269fc6eb2023-11-20T03:30:11ZengMDPI AGMetabolites2218-19892020-06-0110624210.3390/metabo10060242Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat PlasmaVéronique de Bruijn0Christina Behr1Saskia Sperber2Tilmann Walk3Philipp Ternes4Markus Slopianka5Volker Haake6Karsten Beekmann7Bennard van Ravenzwaay8BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, GermanyBASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, GermanyBASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, GermanyBASF Metabolome Solutions, Tegeler Weg 33, 10589 Berlin, GermanyBASF Metabolome Solutions, Tegeler Weg 33, 10589 Berlin, GermanyBASF Metabolome Solutions, Tegeler Weg 33, 10589 Berlin, GermanyBASF Metabolome Solutions, Tegeler Weg 33, 10589 Berlin, GermanyDivision of Toxicology, Wageningen University and Research, 6708 WE Wageningen, The NetherlandsBASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, GermanyVarious environmental factors can alter the gut microbiome’s composition and functionality, and modulate host health. In this study, the effects of oral and parenteral administration of two poorly bioavailable antibiotics (i.e., vancomycin and streptomycin) on male Wistar Crl/Wi(Han) rats for 28 days were compared to distinguish between microbiome-derived or -associated and systemic changes in the plasma metabolome. The resulting changes in the plasma metabolome were compared to the effects of a third reference compound, roxithromycin, which is readily bioavailable. A community analysis revealed that the oral administration of vancomycin and roxithromycin in particular leads to an altered microbial population. Antibiotic-induced changes depending on the administration routes were observed in plasma metabolite levels. Indole-3-acetic acid (IAA) and hippuric acid (HA) were identified as key metabolites of microbiome modulation, with HA being the most sensitive. Even though large variations in the plasma bile acid pool between and within rats were observed, the change in microbiome community was observed to alter the composition of the bile acid pool, especially by an accumulation of taurine-conjugated primary bile acids. In-depth investigation of the relationship between microbiome variability and their functionality, with emphasis on the bile acid pool, will be necessary to better assess the potential adverseness of environmentally induced microbiome changes.https://www.mdpi.com/2218-1989/10/6/242microbiomebile acidsmetabolomicsantibiotics
spellingShingle Véronique de Bruijn
Christina Behr
Saskia Sperber
Tilmann Walk
Philipp Ternes
Markus Slopianka
Volker Haake
Karsten Beekmann
Bennard van Ravenzwaay
Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma
Metabolites
microbiome
bile acids
metabolomics
antibiotics
title Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma
title_full Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma
title_fullStr Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma
title_full_unstemmed Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma
title_short Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma
title_sort antibiotic induced changes in microbiome related metabolites and bile acids in rat plasma
topic microbiome
bile acids
metabolomics
antibiotics
url https://www.mdpi.com/2218-1989/10/6/242
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AT tilmannwalk antibioticinducedchangesinmicrobiomerelatedmetabolitesandbileacidsinratplasma
AT philippternes antibioticinducedchangesinmicrobiomerelatedmetabolitesandbileacidsinratplasma
AT markusslopianka antibioticinducedchangesinmicrobiomerelatedmetabolitesandbileacidsinratplasma
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