Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography
X-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
International Union of Crystallography
2021-11-01
|
| Series: | IUCrJ |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S2052252521008423 |
| _version_ | 1798030208082640896 |
|---|---|
| author | Selina L. S. Storm Danny Axford Robin L. Owen |
| author_facet | Selina L. S. Storm Danny Axford Robin L. Owen |
| author_sort | Selina L. S. Storm |
| collection | DOAJ |
| description | X-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provide an extension of crystal lifetime via an increase in diffraction efficiency. To date, however, hardware limitations have negated any possible gains. Through the first use of a cadmium telluride EIGER2 detector and a beamline optimized for high-energy data collection, it is shown that at higher energies fewer crystals will be required to obtain complete data, as the diffracted intensity per unit dose increases by a factor of more than two between 12.4 and 25 keV. Additionally, these higher energy data can provide more information, as shown by a systematic increase in the high-resolution cutoff of the data collected. Taken together, these gains point to a high-energy future for synchrotron-based macromolecular crystallography. |
| first_indexed | 2024-04-11T19:37:35Z |
| format | Article |
| id | doaj.art-a9b61fdbda464a97ac4b18878e62cd8c |
| institution | Directory Open Access Journal |
| issn | 2052-2525 |
| language | English |
| last_indexed | 2024-04-11T19:37:35Z |
| publishDate | 2021-11-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | IUCrJ |
| spelling | doaj.art-a9b61fdbda464a97ac4b18878e62cd8c2022-12-22T04:06:48ZengInternational Union of CrystallographyIUCrJ2052-25252021-11-018689690410.1107/S2052252521008423jt5058Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallographySelina L. S. Storm0Danny Axford1Robin L. Owen2Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United KingdomDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United KingdomDiamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United KingdomX-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provide an extension of crystal lifetime via an increase in diffraction efficiency. To date, however, hardware limitations have negated any possible gains. Through the first use of a cadmium telluride EIGER2 detector and a beamline optimized for high-energy data collection, it is shown that at higher energies fewer crystals will be required to obtain complete data, as the diffracted intensity per unit dose increases by a factor of more than two between 12.4 and 25 keV. Additionally, these higher energy data can provide more information, as shown by a systematic increase in the high-resolution cutoff of the data collected. Taken together, these gains point to a high-energy future for synchrotron-based macromolecular crystallography.http://scripts.iucr.org/cgi-bin/paper?S2052252521008423high-energy x-raysmacromolecular crystallographyx-ray radiation damage |
| spellingShingle | Selina L. S. Storm Danny Axford Robin L. Owen Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography IUCrJ high-energy x-rays macromolecular crystallography x-ray radiation damage |
| title | Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography |
| title_full | Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography |
| title_fullStr | Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography |
| title_full_unstemmed | Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography |
| title_short | Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography |
| title_sort | experimental evidence for the benefits of higher x ray energies for macromolecular crystallography |
| topic | high-energy x-rays macromolecular crystallography x-ray radiation damage |
| url | http://scripts.iucr.org/cgi-bin/paper?S2052252521008423 |
| work_keys_str_mv | AT selinalsstorm experimentalevidenceforthebenefitsofhigherxrayenergiesformacromolecularcrystallography AT dannyaxford experimentalevidenceforthebenefitsofhigherxrayenergiesformacromolecularcrystallography AT robinlowen experimentalevidenceforthebenefitsofhigherxrayenergiesformacromolecularcrystallography |