Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts
The biochemical mechanism(s) underlying brunescent cataracts remain unclear. Oxidative stress due to reactive oxygen species may have a role in the pigmentation process in eye lens. We have analysed human cataractous lenses for flavins by high-performance liquid chromatography (HPLC), since flavins...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
1998-01-01
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| Series: | Indian Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=1998;volume=46;issue=4;spage=233;epage=237;aulast=Bhat |
| _version_ | 1819131242812014592 |
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| author | Bhat K Nayak Sujatha |
| author_facet | Bhat K Nayak Sujatha |
| author_sort | Bhat K |
| collection | DOAJ |
| description | The biochemical mechanism(s) underlying brunescent cataracts remain unclear. Oxidative stress due to reactive oxygen species may have a role in the pigmentation process in eye lens. We have analysed human cataractous lenses for flavins by high-performance liquid chromatography (HPLC), since flavins are light sensitive and act as endogenous sensitizers generating reactive oxygen species in the eye. The most significant observation in this study is that higher levels of flavin nucleotides occur in brown lens compared to yellow lens. The concentration of flavin nucleotides (flavin monouncleotide, FMN + flavin adenine dinucleotide, FAD) was highest in the nuclear region of the lens followed by the cortical and capsule-epithelial regions. However, the ratio of FAD/FMN was lowest in the nuclear region of the lens followed by other regions. On the other hand, riboflavin was not detected in any of the lens (cataractous) regions. These results suggest that the observed increase in flavin nucleotides in the ocular tissue could contribute towards deepening of lens pigmentation. |
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| format | Article |
| id | doaj.art-a9b655e3a0984f7b8eb73579224081bd |
| institution | Directory Open Access Journal |
| issn | 0301-4738 |
| language | English |
| last_indexed | 2024-12-22T09:12:24Z |
| publishDate | 1998-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-a9b655e3a0984f7b8eb73579224081bd2022-12-21T18:31:24ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-01-01464233237Flavin nucleotides in human lens: Regional distribution in Brunescent cataractsBhat KNayak SujathaThe biochemical mechanism(s) underlying brunescent cataracts remain unclear. Oxidative stress due to reactive oxygen species may have a role in the pigmentation process in eye lens. We have analysed human cataractous lenses for flavins by high-performance liquid chromatography (HPLC), since flavins are light sensitive and act as endogenous sensitizers generating reactive oxygen species in the eye. The most significant observation in this study is that higher levels of flavin nucleotides occur in brown lens compared to yellow lens. The concentration of flavin nucleotides (flavin monouncleotide, FMN + flavin adenine dinucleotide, FAD) was highest in the nuclear region of the lens followed by the cortical and capsule-epithelial regions. However, the ratio of FAD/FMN was lowest in the nuclear region of the lens followed by other regions. On the other hand, riboflavin was not detected in any of the lens (cataractous) regions. These results suggest that the observed increase in flavin nucleotides in the ocular tissue could contribute towards deepening of lens pigmentation.http://www.ijo.in/article.asp?issn=0301-4738;year=1998;volume=46;issue=4;spage=233;epage=237;aulast=BhatFlavin nucleotidesbrunescent cataracthuman lenslens pigmentationlens regions |
| spellingShingle | Bhat K Nayak Sujatha Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts Indian Journal of Ophthalmology Flavin nucleotides brunescent cataract human lens lens pigmentation lens regions |
| title | Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts |
| title_full | Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts |
| title_fullStr | Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts |
| title_full_unstemmed | Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts |
| title_short | Flavin nucleotides in human lens: Regional distribution in Brunescent cataracts |
| title_sort | flavin nucleotides in human lens regional distribution in brunescent cataracts |
| topic | Flavin nucleotides brunescent cataract human lens lens pigmentation lens regions |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=1998;volume=46;issue=4;spage=233;epage=237;aulast=Bhat |
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