Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants
Osteoarthritis is characterized by cartilage loss resulting from the activation of chondrocytes associated with a synovial inflammation. Activated chondrocytes promote an increased secretion of matrix proteases and proinflammatory cytokines leading to cartilage breakdown. Since natural products poss...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/2/245 |
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| author | Morgane Bourmaud Mylene Zarka Romain Le Cozannet Pascale Fança-Berthon Eric Hay Martine Cohen-Solal |
| author_facet | Morgane Bourmaud Mylene Zarka Romain Le Cozannet Pascale Fança-Berthon Eric Hay Martine Cohen-Solal |
| author_sort | Morgane Bourmaud |
| collection | DOAJ |
| description | Osteoarthritis is characterized by cartilage loss resulting from the activation of chondrocytes associated with a synovial inflammation. Activated chondrocytes promote an increased secretion of matrix proteases and proinflammatory cytokines leading to cartilage breakdown. Since natural products possess anti-inflammatory properties, we investigated the direct effect of <i>Rubus idaeus</i> extracts (RIE) in chondrocyte metabolism and cartilage loss. The effect of RIE in chondrocyte metabolism was analyzed in murine primary chondrocytes and cartilage explants. We also assessed the contribution of RIE in an inflammation environment by culturing mice primary chondrocytes with the supernatant of Raw 264.7 macrophage-like cells primed with RIE. In primary chondrocytes, RIE diminished chondrocyte hypertrophy (<i>Col10</i>), while increasing the expression of catabolic genes (<i>Mmp-3</i>, <i>Mmp-13)</i> and reducing anabolic genes (<i>Col2a1</i>, <i>Acan</i>). In cartilage explants, <i>Rubus idaeus</i> prevented the loss of proteoglycan (14.84 ± 3.07% loss of proteoglycans with IL1 alone vs. 3.03 ± 1.86% with IL1 and 100 µg/mL of RIE), as well as the NITEGE neoepitope expression. RIE alone reduced the expression of <i>Il1</i> and <i>Il6</i> in macrophages, without changes in <i>Tnf</i> and <i>Cox2</i> expression. The secretome of macrophages pre-treated with RIE and transferred to chondrocytes decreases the gene and protein expression of <i>Mmp-3</i> and <i>Cox2</i>. In conclusion, these data suggest that RIE may protect from chondrocyte catabolism and cartilage loss in inflammatory conditions. Further evaluations are need before considering RIE as a candidate for the treatment for osteoarthritis. |
| first_indexed | 2024-03-09T05:03:02Z |
| format | Article |
| id | doaj.art-a9b78682160f4e49a48fc1e8b2eef06f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T05:03:02Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a9b78682160f4e49a48fc1e8b2eef06f2023-12-03T12:58:10ZengMDPI AGBiomolecules2218-273X2021-02-0111224510.3390/biom11020245Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and ExplantsMorgane Bourmaud0Mylene Zarka1Romain Le Cozannet2Pascale Fança-Berthon3Eric Hay4Martine Cohen-Solal5BIOSCAR Inserm U1132, Department of Rheumatology, Université de Paris, Hôpital Lariboisière, F-75010 Paris, FranceBIOSCAR Inserm U1132, Department of Rheumatology, Université de Paris, Hôpital Lariboisière, F-75010 Paris, FranceNaturex, Part of Givaudan SA, 250 rue Pierre Bayle, 84000 Avignon, FranceNaturex, Part of Givaudan SA, 250 rue Pierre Bayle, 84000 Avignon, FranceBIOSCAR Inserm U1132, Department of Rheumatology, Université de Paris, Hôpital Lariboisière, F-75010 Paris, FranceBIOSCAR Inserm U1132, Department of Rheumatology, Université de Paris, Hôpital Lariboisière, F-75010 Paris, FranceOsteoarthritis is characterized by cartilage loss resulting from the activation of chondrocytes associated with a synovial inflammation. Activated chondrocytes promote an increased secretion of matrix proteases and proinflammatory cytokines leading to cartilage breakdown. Since natural products possess anti-inflammatory properties, we investigated the direct effect of <i>Rubus idaeus</i> extracts (RIE) in chondrocyte metabolism and cartilage loss. The effect of RIE in chondrocyte metabolism was analyzed in murine primary chondrocytes and cartilage explants. We also assessed the contribution of RIE in an inflammation environment by culturing mice primary chondrocytes with the supernatant of Raw 264.7 macrophage-like cells primed with RIE. In primary chondrocytes, RIE diminished chondrocyte hypertrophy (<i>Col10</i>), while increasing the expression of catabolic genes (<i>Mmp-3</i>, <i>Mmp-13)</i> and reducing anabolic genes (<i>Col2a1</i>, <i>Acan</i>). In cartilage explants, <i>Rubus idaeus</i> prevented the loss of proteoglycan (14.84 ± 3.07% loss of proteoglycans with IL1 alone vs. 3.03 ± 1.86% with IL1 and 100 µg/mL of RIE), as well as the NITEGE neoepitope expression. RIE alone reduced the expression of <i>Il1</i> and <i>Il6</i> in macrophages, without changes in <i>Tnf</i> and <i>Cox2</i> expression. The secretome of macrophages pre-treated with RIE and transferred to chondrocytes decreases the gene and protein expression of <i>Mmp-3</i> and <i>Cox2</i>. In conclusion, these data suggest that RIE may protect from chondrocyte catabolism and cartilage loss in inflammatory conditions. Further evaluations are need before considering RIE as a candidate for the treatment for osteoarthritis.https://www.mdpi.com/2218-273X/11/2/245<i>Rubus idaeus</i>chondrocytesmacrophagesosteoarthritisinflammation |
| spellingShingle | Morgane Bourmaud Mylene Zarka Romain Le Cozannet Pascale Fança-Berthon Eric Hay Martine Cohen-Solal Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants Biomolecules <i>Rubus idaeus</i> chondrocytes macrophages osteoarthritis inflammation |
| title | Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants |
| title_full | Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants |
| title_fullStr | Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants |
| title_full_unstemmed | Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants |
| title_short | Effect of <i>Rubus idaeus</i> Extracts in Murine Chondrocytes and Explants |
| title_sort | effect of i rubus idaeus i extracts in murine chondrocytes and explants |
| topic | <i>Rubus idaeus</i> chondrocytes macrophages osteoarthritis inflammation |
| url | https://www.mdpi.com/2218-273X/11/2/245 |
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