Genome-Wide Identification and Drought Stress Response Pattern of the NF-Y Gene Family in <i>Cymbidium sinense</i>
<i>Cymbidium sinense,</i> a type of orchid plant, is more drought-resistant and ornamental than other terrestrial orchids. Research has shown that many members of the <i>NUCLEAR FACTOR Y (NF-Y)</i> transcription factor family are responsive to plant growth, development, and a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/5/3031 |
| Summary: | <i>Cymbidium sinense,</i> a type of orchid plant, is more drought-resistant and ornamental than other terrestrial orchids. Research has shown that many members of the <i>NUCLEAR FACTOR Y (NF-Y)</i> transcription factor family are responsive to plant growth, development, and abiotic stress. However, the mechanism of the <i>NF-Y</i> gene family’s response to abiotic stress in orchids has not yet been reported. In this study, phylogenetic analysis allowed for 27 <i>CsNF-Y</i> genes to be identified (5 <i>CsNF-YAs</i>, 9 <i>CsNF-YBs</i>, and 13 <i>CsNF-YC</i> subunits), and the <i>CsNF-Y</i>s were homologous to those in <i>Arabidopsis</i> and <i>Oryza</i>. Protein structure analysis revealed that different subfamilies contained different motifs, but all of them contained Motif 2. Secondary and tertiary protein structure analysis indicated that the <i>CsNF-YB</i> and <i>CsNF-YC</i> subfamilies had a high content of alpha helix structures. <i>Cis</i>-element analysis showed that elements related to drought stress were mainly concentrated in the <i>CsNF-YB</i> and <i>CsNF-YC</i> subfamilies, with <i>CsNF-YB3</i> and <i>CsNF-YC12</i> having the highest content. The results of a transcriptome analysis showed that there was a trend of downregulation of almost all <i>CsNF-Ys</i> in leaves under drought stress, while in roots, most members of the <i>CsNF-YB</i> subfamily showed a trend of upregulation. Additionally, seven genes were selected for real-time reverse transcription quantitative PCR (qRT-PCR) experiments. The results were generally consistent with those of the transcriptome analysis. The regulatory roles of <i>CsNF-YB 1</i>, <i>2</i>, and <i>4</i> were particularly evident in the roots. The findings of our study may make a great contribution to the understanding of the role of <i>CsNF-Ys</i> in stress-related metabolic processes. |
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| ISSN: | 1661-6596 1422-0067 |