Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation
Abstract Background Cucumis prophetarum var. prophetarum is used in Saudi folk medicine for treating liver disorders and grows widely between Abha and Khamis Mushait City, Saudi Arabia. Methods Bioassay-guided fractionation and purification were used to isolate the main active constituents of Cucumi...
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2295-5 |
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| author | Abdulrhman Alsayari Lucas Kopel Mahmoud Salama Ahmed Hesham S. M. Soliman Sivakumar Annadurai Fathi T. Halaweish |
| author_facet | Abdulrhman Alsayari Lucas Kopel Mahmoud Salama Ahmed Hesham S. M. Soliman Sivakumar Annadurai Fathi T. Halaweish |
| author_sort | Abdulrhman Alsayari |
| collection | DOAJ |
| description | Abstract Background Cucumis prophetarum var. prophetarum is used in Saudi folk medicine for treating liver disorders and grows widely between Abha and Khamis Mushait City, Saudi Arabia. Methods Bioassay-guided fractionation and purification were used to isolate the main active constituents of Cucumis prophetarum var. prophetarum fruits. These compounds were structurally elucidated using NMR spectroscopy, mass spectral analyses and x-ray crystallography. All fractions, sub-fractions and pure compounds were screened for their anticancer activity against six cancer cell lines. Results The greatest cytotoxic activity was found to be in the ethyl acetate fraction, resulting in the isolation of five cucurbitacin compounds [E, B, D, F-25 acetate and Hexanorcucurbitacin D]. Among the cucurbitacins that were isolated and tested cucurbitacin B and E showed potent cytotoxicity activities against all six human cancer cell lines. Conclusion Human breast cancer cell lines were found to be the most sensitive to cucurbitacins. Preliminary structure activity relationship (SAR) for cytotoxic activity of Cucurbitacins against human breast cancer cell line MDA-MB-231 has been reported. |
| first_indexed | 2024-12-21T17:53:15Z |
| format | Article |
| id | doaj.art-a9b840c78cec4aaa91639bb1eeb410d1 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-21T17:53:15Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-a9b840c78cec4aaa91639bb1eeb410d12022-12-21T18:55:17ZengBMCBMC Complementary and Alternative Medicine1472-68822018-10-0118111210.1186/s12906-018-2295-5Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionationAbdulrhman Alsayari0Lucas Kopel1Mahmoud Salama Ahmed2Hesham S. M. Soliman3Sivakumar Annadurai4Fathi T. Halaweish5Department of Pharmacognosy, College of Pharmacy, King Khalid UniversityKalexsynDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The British University in EgyptDepartment of Pharmacognosy, Helwan UniversityDepartment of Pharmacognosy, College of Pharmacy, King Khalid UniversityDepartment of Chemistry and Biochemistry, South Dakota State UniversityAbstract Background Cucumis prophetarum var. prophetarum is used in Saudi folk medicine for treating liver disorders and grows widely between Abha and Khamis Mushait City, Saudi Arabia. Methods Bioassay-guided fractionation and purification were used to isolate the main active constituents of Cucumis prophetarum var. prophetarum fruits. These compounds were structurally elucidated using NMR spectroscopy, mass spectral analyses and x-ray crystallography. All fractions, sub-fractions and pure compounds were screened for their anticancer activity against six cancer cell lines. Results The greatest cytotoxic activity was found to be in the ethyl acetate fraction, resulting in the isolation of five cucurbitacin compounds [E, B, D, F-25 acetate and Hexanorcucurbitacin D]. Among the cucurbitacins that were isolated and tested cucurbitacin B and E showed potent cytotoxicity activities against all six human cancer cell lines. Conclusion Human breast cancer cell lines were found to be the most sensitive to cucurbitacins. Preliminary structure activity relationship (SAR) for cytotoxic activity of Cucurbitacins against human breast cancer cell line MDA-MB-231 has been reported.http://link.springer.com/article/10.1186/s12906-018-2295-5Bioassay-guided fractionationCucurbitacinsCucumis prophetarum var. prophetarumAnticancerBreast cancerPreliminary SAR |
| spellingShingle | Abdulrhman Alsayari Lucas Kopel Mahmoud Salama Ahmed Hesham S. M. Soliman Sivakumar Annadurai Fathi T. Halaweish Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation BMC Complementary and Alternative Medicine Bioassay-guided fractionation Cucurbitacins Cucumis prophetarum var. prophetarum Anticancer Breast cancer Preliminary SAR |
| title | Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation |
| title_full | Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation |
| title_fullStr | Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation |
| title_full_unstemmed | Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation |
| title_short | Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation |
| title_sort | isolation of anticancer constituents from cucumis prophetarum var prophetarum through bioassay guided fractionation |
| topic | Bioassay-guided fractionation Cucurbitacins Cucumis prophetarum var. prophetarum Anticancer Breast cancer Preliminary SAR |
| url | http://link.springer.com/article/10.1186/s12906-018-2295-5 |
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