Development of an immunocolloidal strip for rapid detection of picoxystrobin
In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1754344 |
| _version_ | 1819203686829654016 |
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| author | Shanshan Song Ziwen Chen Jie Liu Liguang Xu Hua Kuang Jianping Zhu Liqiang Liu |
| author_facet | Shanshan Song Ziwen Chen Jie Liu Liguang Xu Hua Kuang Jianping Zhu Liqiang Liu |
| author_sort | Shanshan Song |
| collection | DOAJ |
| description | In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC50 value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples. |
| first_indexed | 2024-12-23T04:23:52Z |
| format | Article |
| id | doaj.art-a9bd6407f7144c9ab7be48ac89d6f6af |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-23T04:23:52Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-a9bd6407f7144c9ab7be48ac89d6f6af2022-12-21T18:00:11ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131171172210.1080/09540105.2020.17543441754344Development of an immunocolloidal strip for rapid detection of picoxystrobinShanshan Song0Ziwen Chen1Jie Liu2Liguang Xu3Hua Kuang4Jianping Zhu5Liqiang Liu6State Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityIn this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC50 value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples.http://dx.doi.org/10.1080/09540105.2020.1754344picoxystrobinic-elisamonoclonal antibodyimmunochromatographic strips (ics) |
| spellingShingle | Shanshan Song Ziwen Chen Jie Liu Liguang Xu Hua Kuang Jianping Zhu Liqiang Liu Development of an immunocolloidal strip for rapid detection of picoxystrobin Food and Agricultural Immunology picoxystrobin ic-elisa monoclonal antibody immunochromatographic strips (ics) |
| title | Development of an immunocolloidal strip for rapid detection of picoxystrobin |
| title_full | Development of an immunocolloidal strip for rapid detection of picoxystrobin |
| title_fullStr | Development of an immunocolloidal strip for rapid detection of picoxystrobin |
| title_full_unstemmed | Development of an immunocolloidal strip for rapid detection of picoxystrobin |
| title_short | Development of an immunocolloidal strip for rapid detection of picoxystrobin |
| title_sort | development of an immunocolloidal strip for rapid detection of picoxystrobin |
| topic | picoxystrobin ic-elisa monoclonal antibody immunochromatographic strips (ics) |
| url | http://dx.doi.org/10.1080/09540105.2020.1754344 |
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