Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys
ABSTRACT Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurus is the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are...
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Academia Brasileira de Ciências
2016-01-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200665&lng=en&tlng=en |
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| author | NATHALIA A. SILVA CLEIDE M.R. ALBUQUERQUE ALINE D. MARINHO ROBERTA J.B. JORGE ANTONIO G. SILVA NETO HELENA S.A. MONTEIRO TÚLIO D. SILVA MÁRCIA V. SILVA MARIA TEREZA S. CORREIA TICIANA P. PEREIRA ALICE M.C. MARTINS DALGIMAR B. MENEZES RAFAEL M. XIMENES RENÉ D. MARTINS |
| author_facet | NATHALIA A. SILVA CLEIDE M.R. ALBUQUERQUE ALINE D. MARINHO ROBERTA J.B. JORGE ANTONIO G. SILVA NETO HELENA S.A. MONTEIRO TÚLIO D. SILVA MÁRCIA V. SILVA MARIA TEREZA S. CORREIA TICIANA P. PEREIRA ALICE M.C. MARTINS DALGIMAR B. MENEZES RAFAEL M. XIMENES RENÉ D. MARTINS |
| author_sort | NATHALIA A. SILVA |
| collection | DOAJ |
| description | ABSTRACT Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurus is the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurus venom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), and electrolyte excretion were analyzed. Effects were observed only at TsV concentration of 1.0 μg/mL, which increased PP (controlPP40' = 92.7 ± 1.95; TsVPP40' = 182.0 ± 4.70* mmHg, *p < 0.05), RVR (controlRVR40' = 3.28 ± 0.23 mmHg; TstRVR40' = 6.76 ± 0.45* mmHg, *p < 0.05), UF (controlUF50' = 0.16 ± 0.04; TstUF50' = 0.60 ± 0.10* mL/g/min,*p < 0.05), GFR and electrolyte excretion, with histological changes that indicate renal tubular injury. In conclusion, T. stigmurus venom induces a transient increase in PP with tubular injury, both of which lead to an augmented electrolyte excretion. |
| first_indexed | 2024-04-13T02:03:03Z |
| format | Article |
| id | doaj.art-a9be322ef5b843b394b2d3bcb6bb10e2 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-13T02:03:03Z |
| publishDate | 2016-01-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-a9be322ef5b843b394b2d3bcb6bb10e22022-12-22T03:07:34ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902016-01-0188suppl 166567510.1590/0001-3765201620150253S0001-37652016000200665Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneysNATHALIA A. SILVACLEIDE M.R. ALBUQUERQUEALINE D. MARINHOROBERTA J.B. JORGEANTONIO G. SILVA NETOHELENA S.A. MONTEIROTÚLIO D. SILVAMÁRCIA V. SILVAMARIA TEREZA S. CORREIATICIANA P. PEREIRAALICE M.C. MARTINSDALGIMAR B. MENEZESRAFAEL M. XIMENESRENÉ D. MARTINSABSTRACT Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurus is the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurus venom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), and electrolyte excretion were analyzed. Effects were observed only at TsV concentration of 1.0 μg/mL, which increased PP (controlPP40' = 92.7 ± 1.95; TsVPP40' = 182.0 ± 4.70* mmHg, *p < 0.05), RVR (controlRVR40' = 3.28 ± 0.23 mmHg; TstRVR40' = 6.76 ± 0.45* mmHg, *p < 0.05), UF (controlUF50' = 0.16 ± 0.04; TstUF50' = 0.60 ± 0.10* mL/g/min,*p < 0.05), GFR and electrolyte excretion, with histological changes that indicate renal tubular injury. In conclusion, T. stigmurus venom induces a transient increase in PP with tubular injury, both of which lead to an augmented electrolyte excretion.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200665&lng=en&tlng=en2D-PAGErimMDCKvenenoTityus stigmurus |
| spellingShingle | NATHALIA A. SILVA CLEIDE M.R. ALBUQUERQUE ALINE D. MARINHO ROBERTA J.B. JORGE ANTONIO G. SILVA NETO HELENA S.A. MONTEIRO TÚLIO D. SILVA MÁRCIA V. SILVA MARIA TEREZA S. CORREIA TICIANA P. PEREIRA ALICE M.C. MARTINS DALGIMAR B. MENEZES RAFAEL M. XIMENES RENÉ D. MARTINS Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys Anais da Academia Brasileira de Ciências 2D-PAGE rim MDCK veneno Tityus stigmurus |
| title | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys |
| title_full | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys |
| title_fullStr | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys |
| title_full_unstemmed | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys |
| title_short | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys |
| title_sort | effects of tityus stigmurus thorell 1876 scorpiones buthidae venom in isolated perfused rat kidneys |
| topic | 2D-PAGE rim MDCK veneno Tityus stigmurus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200665&lng=en&tlng=en |
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