Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil
ABSTRACT: Objectives: The draft genome sequence of Campylobacter jejuni (Cj26) was analysed to investigate genetic mechanisms of antimicrobial resistance, virulence-associated genes, and phylogenetic context. Methods: Antimicrobial resistance was assessed by agar dilution and disk diffusion. Cj26 w...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652300084X |
| _version_ | 1797689515068882944 |
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| author | Thomas Salles Dias Pedro Panzenhagen Arthur de Almeida Figueira Gisllany Alves Costa Daise Aparecida Rossi Roberta Torres de Melo Virginia Léo de Almeida Pereira Maria Helena Cosendey de Aquino |
| author_facet | Thomas Salles Dias Pedro Panzenhagen Arthur de Almeida Figueira Gisllany Alves Costa Daise Aparecida Rossi Roberta Torres de Melo Virginia Léo de Almeida Pereira Maria Helena Cosendey de Aquino |
| author_sort | Thomas Salles Dias |
| collection | DOAJ |
| description | ABSTRACT: Objectives: The draft genome sequence of Campylobacter jejuni (Cj26) was analysed to investigate genetic mechanisms of antimicrobial resistance, virulence-associated genes, and phylogenetic context. Methods: Antimicrobial resistance was assessed by agar dilution and disk diffusion. Cj26 was sequenced using NovaSeq 6000 technology. The genome was assembled and annotated. Resistance genes and chromosomal mutations were analysed using the Center for Genomic Epidemiology services, and the multilocus sequence type, SVR-flaA, and porA were determined. The virulome was determined using the Virulence Factor Database. Plasmid detection and assembly were performed using Unicycler v0.5.0 software. To infer the core genome phylogeny, prokka v1.14.5 was employed in conjunction with IQtree v2.0.3. Results: The Cj26 strain showed a high level of resistance to ciprofloxacin (32 µg/mL) and erythromycin (>128 µg/mL) and resistance to tetracycline and ampicillin. Multilocus sequence typing revealed that the strain belonged to sequence type 353. The substitutions Tre-86-Ile in gyrA and A2075G in 23s RNA were detected, along with the genes tetO, aph(3′)-III, ant(6)-Ia, and blaOXA 460. A consistent relationship among accessory and core genes was identified. When compared to other sequence type 353 genomes from Brazil, Cj26 clustered with strains that had more antimicrobial resistance genes than the other clusters. Conclusion: This report provides insight into the antimicrobial resistance determinants found in a C. jejuni strain and offers a valuable resource for further studies on Campylobacter genomics and antimicrobial resistance. |
| first_indexed | 2024-03-12T01:46:39Z |
| format | Article |
| id | doaj.art-a9beb54bc3af4f129311009280cf134e |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-03-12T01:46:39Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-a9beb54bc3af4f129311009280cf134e2023-09-09T04:55:07ZengElsevierJournal of Global Antimicrobial Resistance2213-71652023-09-013414Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern BrazilThomas Salles Dias0Pedro Panzenhagen1Arthur de Almeida Figueira2Gisllany Alves Costa3Daise Aparecida Rossi4Roberta Torres de Melo5Virginia Léo de Almeida Pereira6Maria Helena Cosendey de Aquino7Postgraduate Program in Veterinary Medicine (Veterinary Hygiene and Processing Technology of Animal Products), Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, Brazil; Corresponding author. Mailing address: Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brasil Filho 64, Niteroi, Rio de Janeiro 24230340, Brazil.Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, Brazil; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, BrazilPostgraduate Program in Veterinary Medicine (Veterinary Hygiene and Processing Technology of Animal Products), Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, BrazilPostgraduate Program in Veterinary Medicine (Veterinary Hygiene and Processing Technology of Animal Products), Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, BrazilLaboratory of Molecular Epidemiology, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia, BrazilLaboratory of Molecular Epidemiology, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia, BrazilPostgraduate Program in Veterinary Medicine (Veterinary Hygiene and Processing Technology of Animal Products), Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, Brazil; Department of Preventive Veterinary Medicine, Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, BrazilPostgraduate Program in Veterinary Medicine (Veterinary Hygiene and Processing Technology of Animal Products), Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, Brazil; Department of Preventive Veterinary Medicine, Faculdade de Veterinária, Universidade Federal Fluminense, Niteroi, BrazilABSTRACT: Objectives: The draft genome sequence of Campylobacter jejuni (Cj26) was analysed to investigate genetic mechanisms of antimicrobial resistance, virulence-associated genes, and phylogenetic context. Methods: Antimicrobial resistance was assessed by agar dilution and disk diffusion. Cj26 was sequenced using NovaSeq 6000 technology. The genome was assembled and annotated. Resistance genes and chromosomal mutations were analysed using the Center for Genomic Epidemiology services, and the multilocus sequence type, SVR-flaA, and porA were determined. The virulome was determined using the Virulence Factor Database. Plasmid detection and assembly were performed using Unicycler v0.5.0 software. To infer the core genome phylogeny, prokka v1.14.5 was employed in conjunction with IQtree v2.0.3. Results: The Cj26 strain showed a high level of resistance to ciprofloxacin (32 µg/mL) and erythromycin (>128 µg/mL) and resistance to tetracycline and ampicillin. Multilocus sequence typing revealed that the strain belonged to sequence type 353. The substitutions Tre-86-Ile in gyrA and A2075G in 23s RNA were detected, along with the genes tetO, aph(3′)-III, ant(6)-Ia, and blaOXA 460. A consistent relationship among accessory and core genes was identified. When compared to other sequence type 353 genomes from Brazil, Cj26 clustered with strains that had more antimicrobial resistance genes than the other clusters. Conclusion: This report provides insight into the antimicrobial resistance determinants found in a C. jejuni strain and offers a valuable resource for further studies on Campylobacter genomics and antimicrobial resistance.http://www.sciencedirect.com/science/article/pii/S221371652300084XBrazilMultidrug resistancePoultry |
| spellingShingle | Thomas Salles Dias Pedro Panzenhagen Arthur de Almeida Figueira Gisllany Alves Costa Daise Aparecida Rossi Roberta Torres de Melo Virginia Léo de Almeida Pereira Maria Helena Cosendey de Aquino Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil Journal of Global Antimicrobial Resistance Brazil Multidrug resistance Poultry |
| title | Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil |
| title_full | Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil |
| title_fullStr | Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil |
| title_full_unstemmed | Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil |
| title_short | Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil |
| title_sort | genomic characterisation of campylobacter jejuni cj26 a high level ciprofloxacin erythromycin resistant strain isolated from a poultry carcass in southern brazil |
| topic | Brazil Multidrug resistance Poultry |
| url | http://www.sciencedirect.com/science/article/pii/S221371652300084X |
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