Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram
The aim of this experiment was determine of antibiotic resistance profile of Escherichia coli isolated from rectal swabs of chicken and ram from two different conventional breeding from Slovakia. For the antibiotic susceptibility testing disk diffusion method was used. A tested bacterium, Escherichi...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/680 |
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| author | Jaroslav Pochop Lukáš Hleba Miroslava Kačániová Juraj Čuboň Katarína Rovná Henrieta Arpášová |
| author_facet | Jaroslav Pochop Lukáš Hleba Miroslava Kačániová Juraj Čuboň Katarína Rovná Henrieta Arpášová |
| author_sort | Jaroslav Pochop |
| collection | DOAJ |
| description | The aim of this experiment was determine of antibiotic resistance profile of Escherichia coli isolated from rectal swabs of chicken and ram from two different conventional breeding from Slovakia. For the antibiotic susceptibility testing disk diffusion method was used. A tested bacterium, Escherichia coli was exposed against four antibiotics: amikacin, gentamycin, tetracycline and chloramphenicol. For the identification of this strain, we used Chromogenic coliform agar, Triple sugar iron agar and biochemical test (ENTEROtest 24). For genetic identification of Escherichia coli Step One Real Time PCR with using special primer was used. Was determined that antibiotic resistance in Escherichia coli was not found. Was found susceptibility in all cases of Escherichia coli isolates. Antibiotic resistance is a biological danger. Bacteria, which we study, are considered to reservoirs of resistant genes and they are facultative and obligate pathogens. If these pathogen bacteria cause diseases those these diseases are difficult to treat. In this study, we determined that we have healthy farms in Slovakia too. In this farm antibiotic was not use and we do not determined any resistance to antibiotics, which we used in experiment. |
| first_indexed | 2024-04-24T11:29:08Z |
| format | Article |
| id | doaj.art-a9c2275394b0477fb0e75b29b939d053 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:29:08Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-a9c2275394b0477fb0e75b29b939d0532024-04-10T11:07:19ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01452127127680Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and RamJaroslav Pochop0Lukáš Hleba1Miroslava Kačániová2Juraj Čuboň3Katarína Rovná4Henrieta Arpášová5Deapertment of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 - Nitra, Slovak RepublicDeapertment of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 - Nitra, Slovak RepublicDeapertment of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 - Nitra, Slovak RepublicFaculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 - Nitra, SlovakiaDepartment of Green's Biotechnics, Horticulture and Landscape Engineering Faculty, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 - Nitra, Slovak RepublicDepartment of Poultry Science and Small Animal Husbandry, Faculty of Agrobiology and Food resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 - Nitra, Slovak RepublicThe aim of this experiment was determine of antibiotic resistance profile of Escherichia coli isolated from rectal swabs of chicken and ram from two different conventional breeding from Slovakia. For the antibiotic susceptibility testing disk diffusion method was used. A tested bacterium, Escherichia coli was exposed against four antibiotics: amikacin, gentamycin, tetracycline and chloramphenicol. For the identification of this strain, we used Chromogenic coliform agar, Triple sugar iron agar and biochemical test (ENTEROtest 24). For genetic identification of Escherichia coli Step One Real Time PCR with using special primer was used. Was determined that antibiotic resistance in Escherichia coli was not found. Was found susceptibility in all cases of Escherichia coli isolates. Antibiotic resistance is a biological danger. Bacteria, which we study, are considered to reservoirs of resistant genes and they are facultative and obligate pathogens. If these pathogen bacteria cause diseases those these diseases are difficult to treat. In this study, we determined that we have healthy farms in Slovakia too. In this farm antibiotic was not use and we do not determined any resistance to antibiotics, which we used in experiment.https://spasb.ro/index.php/public_html/article/view/680antibiotic resistanceescherichia colichickenramrectal swabs |
| spellingShingle | Jaroslav Pochop Lukáš Hleba Miroslava Kačániová Juraj Čuboň Katarína Rovná Henrieta Arpášová Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram Scientific Papers Animal Science and Biotechnologies antibiotic resistance escherichia coli chicken ram rectal swabs |
| title | Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram |
| title_full | Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram |
| title_fullStr | Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram |
| title_full_unstemmed | Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram |
| title_short | Healthy Farms in Slovakia: Antibiotic Sensitivity of Escherichia coli Isolated from Rectal Swabs of Chicken and Ram |
| title_sort | healthy farms in slovakia antibiotic sensitivity of escherichia coli isolated from rectal swabs of chicken and ram |
| topic | antibiotic resistance escherichia coli chicken ram rectal swabs |
| url | https://spasb.ro/index.php/public_html/article/view/680 |
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