Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass
For improving enzyme utilization in biotechnological processes, process costs have to be reduced, enzyme stability during industrial processes should be enhanced, and the recycle and reuse step should be favorable. The immobilization of enzymes is an important step for enhancing enzyme catalytic pro...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Slovenian Chemical Society
2019-09-01
|
| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/5013 |
| _version_ | 1818468837478105088 |
|---|---|
| author | Mateja Primožič Gordana Hojnik Podrepšek Irena Pavlovič Mojca Škerget Željko Knez Maja Leitgeb |
| author_facet | Mateja Primožič Gordana Hojnik Podrepšek Irena Pavlovič Mojca Škerget Željko Knez Maja Leitgeb |
| author_sort | Mateja Primožič |
| collection | DOAJ |
| description | For improving enzyme utilization in biotechnological processes, process costs have to be reduced, enzyme stability during industrial processes should be enhanced, and the recycle and reuse step should be favorable. The immobilization of enzymes is an important step for enhancing enzyme catalytic properties and operational stability. In order to reduce the costs of immobilization and consequently the cost of processes, a cheaper carrier (e.g. materials reclaimed as by-products) should be used. To achieve this, cellulase from Trichoderma sp. was immobilized on biochar obtained by low temperature hydrothermal carbonization (LTHTC) in two ways: by adsorption and by covalent binding via a crosslinking agent. The effect of immobilization time, enzyme concentration, type and concentration of the crosslinking agent and the types of carrier - biochar (LTHTC of waste from olive oil production (LTHTC of OL waste) or LTHTC of cellulose) on the immobilization efficiency and the residual activity of biocatalyst was studied. Higher immobilization efficiency and residual enzyme activity was achieved when the enzyme was covalently bound to biochar obtained by LTHTC of cellulose. |
| first_indexed | 2024-04-13T21:16:08Z |
| format | Article |
| id | doaj.art-a9c73b8503be429880dc3471b99917db |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-04-13T21:16:08Z |
| publishDate | 2019-09-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-a9c73b8503be429880dc3471b99917db2022-12-22T02:29:40ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552019-09-0166373273910.17344/acsi.2019.5013731Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of BiomassMateja Primožič0Gordana Hojnik Podrepšek1Irena Pavlovič2Mojca Škerget3Željko Knez4Maja Leitgeb5University of Maribor, Faculty of Chemistry and Chemical EngineeringUniversity of Maribor, Faculty of Chemistry and Chemical EngineeringUniversity of Maribor, Faculty of Chemistry and Chemical EngineeringUniversity of Maribor, Faculty of Chemistry and Chemical EngineeringUniversity of Maribor, Faculty of Chemistry and Chemical Engineering University of Maribor, Faculty of MedicineUniversity of Maribor, Faculty of Chemistry and Chemical Engineering University of Maribor, Faculty of MedicineFor improving enzyme utilization in biotechnological processes, process costs have to be reduced, enzyme stability during industrial processes should be enhanced, and the recycle and reuse step should be favorable. The immobilization of enzymes is an important step for enhancing enzyme catalytic properties and operational stability. In order to reduce the costs of immobilization and consequently the cost of processes, a cheaper carrier (e.g. materials reclaimed as by-products) should be used. To achieve this, cellulase from Trichoderma sp. was immobilized on biochar obtained by low temperature hydrothermal carbonization (LTHTC) in two ways: by adsorption and by covalent binding via a crosslinking agent. The effect of immobilization time, enzyme concentration, type and concentration of the crosslinking agent and the types of carrier - biochar (LTHTC of waste from olive oil production (LTHTC of OL waste) or LTHTC of cellulose) on the immobilization efficiency and the residual activity of biocatalyst was studied. Higher immobilization efficiency and residual enzyme activity was achieved when the enzyme was covalently bound to biochar obtained by LTHTC of cellulose.https://journals.matheo.si/index.php/ACSi/article/view/5013Hydrothermal carbonizationbiocharenzymeimmobilizationenzyme activity. |
| spellingShingle | Mateja Primožič Gordana Hojnik Podrepšek Irena Pavlovič Mojca Škerget Željko Knez Maja Leitgeb Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass Acta Chimica Slovenica Hydrothermal carbonization biochar enzyme immobilization enzyme activity. |
| title | Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass |
| title_full | Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass |
| title_fullStr | Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass |
| title_full_unstemmed | Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass |
| title_short | Enzyme Immobilization Onto Biochar Produced by the Hydrothermal Carbonization of Biomass |
| title_sort | enzyme immobilization onto biochar produced by the hydrothermal carbonization of biomass |
| topic | Hydrothermal carbonization biochar enzyme immobilization enzyme activity. |
| url | https://journals.matheo.si/index.php/ACSi/article/view/5013 |
| work_keys_str_mv | AT matejaprimozic enzymeimmobilizationontobiocharproducedbythehydrothermalcarbonizationofbiomass AT gordanahojnikpodrepsek enzymeimmobilizationontobiocharproducedbythehydrothermalcarbonizationofbiomass AT irenapavlovic enzymeimmobilizationontobiocharproducedbythehydrothermalcarbonizationofbiomass AT mojcaskerget enzymeimmobilizationontobiocharproducedbythehydrothermalcarbonizationofbiomass AT zeljkoknez enzymeimmobilizationontobiocharproducedbythehydrothermalcarbonizationofbiomass AT majaleitgeb enzymeimmobilizationontobiocharproducedbythehydrothermalcarbonizationofbiomass |