A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements
Abstract Background Existing methods for directly measuring photosynthetic capacity (A max) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchange systems are not designed to maximize throughput, on either a cost...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-09-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13007-018-0347-y |
| _version_ | 1819239515509751808 |
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| author | William T. Salter Matthew E. Gilbert Thomas N. Buckley |
| author_facet | William T. Salter Matthew E. Gilbert Thomas N. Buckley |
| author_sort | William T. Salter |
| collection | DOAJ |
| description | Abstract Background Existing methods for directly measuring photosynthetic capacity (A max) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchange systems are not designed to maximize throughput, on either a cost or time basis. Results We present a novel multiplexed semi-portable gas exchange system, OCTOflux, that can measure A max with approximately 4–7 times the throughput of commercial devices, despite a lower capital cost. The main time efficiency arises from having eight leaves simultaneously acclimate to saturating CO2 and high light levels; the long acclimation periods for each leaf (13.8 min on average in this study) thus overlap to a large degree, rather than occurring sequentially. The cost efficiency arises partly from custom-building the system and thus avoiding commercial costs like distribution, marketing and profit, and partly from optimizing the system’s design for A max throughput rather than flexibility for other types of measurements. Conclusion Throughput for A max measurements can be increased greatly, on both a cost and time basis, by multiplexing gas streams from several leaf chambers connected to a single gas analyzer. This can help overcome the bottleneck in breeding and ecological research posed by limited phenotyping throughput for A max. |
| first_indexed | 2024-12-23T13:53:21Z |
| format | Article |
| id | doaj.art-a9c82a6f736a41978d79aef126d5d9b2 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-23T13:53:21Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-a9c82a6f736a41978d79aef126d5d9b22022-12-21T17:44:32ZengBMCPlant Methods1746-48112018-09-0114111210.1186/s13007-018-0347-yA multiplexed gas exchange system for increased throughput of photosynthetic capacity measurementsWilliam T. Salter0Matthew E. Gilbert1Thomas N. Buckley2School of Life and Environmental Sciences, Sydney Institute of Agriculture, The University of SydneyDepartment of Plant Sciences, University of California, DavisDepartment of Plant Sciences, University of California, DavisAbstract Background Existing methods for directly measuring photosynthetic capacity (A max) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchange systems are not designed to maximize throughput, on either a cost or time basis. Results We present a novel multiplexed semi-portable gas exchange system, OCTOflux, that can measure A max with approximately 4–7 times the throughput of commercial devices, despite a lower capital cost. The main time efficiency arises from having eight leaves simultaneously acclimate to saturating CO2 and high light levels; the long acclimation periods for each leaf (13.8 min on average in this study) thus overlap to a large degree, rather than occurring sequentially. The cost efficiency arises partly from custom-building the system and thus avoiding commercial costs like distribution, marketing and profit, and partly from optimizing the system’s design for A max throughput rather than flexibility for other types of measurements. Conclusion Throughput for A max measurements can be increased greatly, on both a cost and time basis, by multiplexing gas streams from several leaf chambers connected to a single gas analyzer. This can help overcome the bottleneck in breeding and ecological research posed by limited phenotyping throughput for A max.http://link.springer.com/article/10.1186/s13007-018-0347-yPhenotypingPhotosynthesisHigh-throughputGas-exchangePhotosynthetic capacityA max |
| spellingShingle | William T. Salter Matthew E. Gilbert Thomas N. Buckley A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements Plant Methods Phenotyping Photosynthesis High-throughput Gas-exchange Photosynthetic capacity A max |
| title | A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements |
| title_full | A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements |
| title_fullStr | A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements |
| title_full_unstemmed | A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements |
| title_short | A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements |
| title_sort | multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements |
| topic | Phenotyping Photosynthesis High-throughput Gas-exchange Photosynthetic capacity A max |
| url | http://link.springer.com/article/10.1186/s13007-018-0347-y |
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