Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it>
<p>Abstract</p> <p>Background</p> <p><it>Bacillus anthracis </it>has two major virulence factors: a tripartite toxin that produces lethal and edema toxins and a polyglutamic acid capsule. A recent report suggested that a toxin belonging to the cholesterol de...
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| Format: | Article |
| Language: | English |
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BMC
2008-09-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/8/159 |
| _version_ | 1818690036748517376 |
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| author | Rest Richard F Rivera Johanna Nakouzi Antonio Casadevall Arturo |
| author_facet | Rest Richard F Rivera Johanna Nakouzi Antonio Casadevall Arturo |
| author_sort | Rest Richard F |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Bacillus anthracis </it>has two major virulence factors: a tripartite toxin that produces lethal and edema toxins and a polyglutamic acid capsule. A recent report suggested that a toxin belonging to the cholesterol dependant cytolysin (CDC) family, anthrolysin O (ALO) was a new virulence factor for <it>B. anthracis </it>but subsequent studies have questioned its relevance in pathogenesis. In this study, we examined the immunogenicity of recombinant anthrolysin O (rALO) in mice.</p> <p>Results</p> <p>BALB/c mice immunized with rALO and boosted after two weeks, produce sera with strong Ab responses with a predominance of IgG1 and IgG2a. Five hybridomas to rALO were recovered representing the IgM, IgG1, and IgG2b isotypes. Passive administration of 3 of the five monoclonal antibodies (mAbs) to rALO prior to infection with lethal intravenous (i.v.) <it>B. anthracis </it>Sterne strain infection in mice was associated with enhanced average survival and a greater likelihood of surviving infection. A combination of two mAbs to ALO was more effective than either mAb separately. One mAb (64F8) slowed the toxicity of rALO for J774.16 macrophage-like cells.</p> <p>Conclusion</p> <p>Our results suggest that ALO contributes to the virulence of <it>B. anthracis </it>Sterne strain in this infection model and that Ab response to ALO may contribute to protection in certain circumstances.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-17T12:19:37Z |
| publishDate | 2008-09-01 |
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| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a9cb915793f54295b71e939bdedec4f02022-12-21T21:49:02ZengBMCBMC Microbiology1471-21802008-09-018115910.1186/1471-2180-8-159Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it>Rest Richard FRivera JohannaNakouzi AntonioCasadevall Arturo<p>Abstract</p> <p>Background</p> <p><it>Bacillus anthracis </it>has two major virulence factors: a tripartite toxin that produces lethal and edema toxins and a polyglutamic acid capsule. A recent report suggested that a toxin belonging to the cholesterol dependant cytolysin (CDC) family, anthrolysin O (ALO) was a new virulence factor for <it>B. anthracis </it>but subsequent studies have questioned its relevance in pathogenesis. In this study, we examined the immunogenicity of recombinant anthrolysin O (rALO) in mice.</p> <p>Results</p> <p>BALB/c mice immunized with rALO and boosted after two weeks, produce sera with strong Ab responses with a predominance of IgG1 and IgG2a. Five hybridomas to rALO were recovered representing the IgM, IgG1, and IgG2b isotypes. Passive administration of 3 of the five monoclonal antibodies (mAbs) to rALO prior to infection with lethal intravenous (i.v.) <it>B. anthracis </it>Sterne strain infection in mice was associated with enhanced average survival and a greater likelihood of surviving infection. A combination of two mAbs to ALO was more effective than either mAb separately. One mAb (64F8) slowed the toxicity of rALO for J774.16 macrophage-like cells.</p> <p>Conclusion</p> <p>Our results suggest that ALO contributes to the virulence of <it>B. anthracis </it>Sterne strain in this infection model and that Ab response to ALO may contribute to protection in certain circumstances.</p>http://www.biomedcentral.com/1471-2180/8/159 |
| spellingShingle | Rest Richard F Rivera Johanna Nakouzi Antonio Casadevall Arturo Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it> BMC Microbiology |
| title | Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it> |
| title_full | Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it> |
| title_fullStr | Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it> |
| title_full_unstemmed | Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it> |
| title_short | Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with <it>Bacillus anthracis</it> |
| title_sort | passive administration of monoclonal antibodies to anthrolysin o prolong survival in mice lethally infected with it bacillus anthracis it |
| url | http://www.biomedcentral.com/1471-2180/8/159 |
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