Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma
There is an obvious need to diagnose hepatocellular carcinoma using novel non-invasive and sensitive biomarkers. In this regard, the aim of this study was to evaluate and correlate both relative quantification of microRNA-7 using quantitative real time polymerase chain reaction and quantitative anal...
| Главные авторы: | , , , , , |
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| Формат: | Статья |
| Язык: | English |
| Опубликовано: |
IOS Press
2017-04-01
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| Серии: | Tumor Biology |
| Online-ссылка: | https://doi.org/10.1177/1010428317698372 |
| _version_ | 1831578526695817216 |
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| author | Marwa Tarek Manal Louis Louka Eman Khairy Randa Ali-Labib Doaa Zakaria Zaky Iman F Montasser |
| author_facet | Marwa Tarek Manal Louis Louka Eman Khairy Randa Ali-Labib Doaa Zakaria Zaky Iman F Montasser |
| author_sort | Marwa Tarek |
| collection | DOAJ |
| description | There is an obvious need to diagnose hepatocellular carcinoma using novel non-invasive and sensitive biomarkers. In this regard, the aim of this study was to evaluate and correlate both relative quantification of microRNA-7 using quantitative real time polymerase chain reaction and quantitative analysis of selenoprotein P using enzyme-linked immunosorbent assay in sera of hepatocellular carcinoma patients, chronic liver disease patients, as well as normal healthy subjects in order to establish a new diagnostic biomarker with a valid non-invasive technique. In addition, this study aimed to investigate whether changes in selenium supply affect microRNA-7 expression and selenoprotein P levels in human hepatocarcinoma cell line (HepG2). The results showed a highly significant decrease in serum microRNA-7 relative quantification values and selenoprotein P levels in malignant group in comparison with benign and control groups. The best cutoff for serum microRNA-7 and selenoprotein P to discriminate hepatocellular carcinoma group from benign and control groups was 0.06 and 4.30 mg/L, respectively. Furthermore, this study showed that changes in selenium supply to HepG2 cell line can alter the microRNA-7 profile and are paralleled by changes in the concentration of its target protein (selenoprotein P). Hence, serum microRNA-7 and selenoprotein P appear to be potential non-invasive diagnostic markers for hepatocellular carcinoma. Moreover, the results suggest that selenium could be used as an anticancer therapy for hepatocellular carcinoma by affecting both microRNA-7 and selenoprotein P. |
| first_indexed | 2024-12-17T16:12:40Z |
| format | Article |
| id | doaj.art-a9cc7a8e2efa4abeb6749cac77a1f7f6 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-17T16:12:40Z |
| publishDate | 2017-04-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-a9cc7a8e2efa4abeb6749cac77a1f7f62022-12-21T21:41:45ZengIOS PressTumor Biology1423-03802017-04-013910.1177/1010428317698372Role of microRNA-7 and selenoprotein P in hepatocellular carcinomaMarwa Tarek0Manal Louis Louka1Eman Khairy2Randa Ali-Labib3Doaa Zakaria Zaky4Iman F Montasser5Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptTropical Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptTropical Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptThere is an obvious need to diagnose hepatocellular carcinoma using novel non-invasive and sensitive biomarkers. In this regard, the aim of this study was to evaluate and correlate both relative quantification of microRNA-7 using quantitative real time polymerase chain reaction and quantitative analysis of selenoprotein P using enzyme-linked immunosorbent assay in sera of hepatocellular carcinoma patients, chronic liver disease patients, as well as normal healthy subjects in order to establish a new diagnostic biomarker with a valid non-invasive technique. In addition, this study aimed to investigate whether changes in selenium supply affect microRNA-7 expression and selenoprotein P levels in human hepatocarcinoma cell line (HepG2). The results showed a highly significant decrease in serum microRNA-7 relative quantification values and selenoprotein P levels in malignant group in comparison with benign and control groups. The best cutoff for serum microRNA-7 and selenoprotein P to discriminate hepatocellular carcinoma group from benign and control groups was 0.06 and 4.30 mg/L, respectively. Furthermore, this study showed that changes in selenium supply to HepG2 cell line can alter the microRNA-7 profile and are paralleled by changes in the concentration of its target protein (selenoprotein P). Hence, serum microRNA-7 and selenoprotein P appear to be potential non-invasive diagnostic markers for hepatocellular carcinoma. Moreover, the results suggest that selenium could be used as an anticancer therapy for hepatocellular carcinoma by affecting both microRNA-7 and selenoprotein P.https://doi.org/10.1177/1010428317698372 |
| spellingShingle | Marwa Tarek Manal Louis Louka Eman Khairy Randa Ali-Labib Doaa Zakaria Zaky Iman F Montasser Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma Tumor Biology |
| title | Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma |
| title_full | Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma |
| title_fullStr | Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma |
| title_full_unstemmed | Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma |
| title_short | Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma |
| title_sort | role of microrna 7 and selenoprotein p in hepatocellular carcinoma |
| url | https://doi.org/10.1177/1010428317698372 |
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