Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer
Abstract Background A few studies have reported the distribution of the microbiota in breast cancer tissues, but few reports have compared the microbiota in different subtypes of breast cancer tissue. Moreover, no study has reported on the relationship between the microbiota and gene expression in b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-10726-4 |
| _version_ | 1797864442129547264 |
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| author | Enuo Liu Fan Zhang Tiansheng Xu Luyi Ye Sean Si Qian Ma Zai-Si Ji |
| author_facet | Enuo Liu Fan Zhang Tiansheng Xu Luyi Ye Sean Si Qian Ma Zai-Si Ji |
| author_sort | Enuo Liu |
| collection | DOAJ |
| description | Abstract Background A few studies have reported the distribution of the microbiota in breast cancer tissues, but few reports have compared the microbiota in different subtypes of breast cancer tissue. Moreover, no study has reported on the relationship between the microbiota and gene expression in breast tumor. Methods Sections of formalin-fixed paraffin-embedded (FFPE) tissue were prepared from the breast tumors of 70 patients and were subjected to microarray analysis to identify gene expression profiles. The same total RNA samples were also used to analyze the microbiota activity in tumor tissues by performing 16 S rRNA sequencing and internal transcribed spacer (ITS) sequencing of reverse transcript cDNA with Illumina Miseq. Pearson’s correlation coefficient was used for calculating the correlation between microbial relative activity and gene expression. Results The microbiota transcriptional activity of 70 FFPE samples mainly consisted of the phyla Bacteroidetes, Firmicutes and Proteobacteria. Prevotella_9, Bacteroides and Alloprevotella were the most active genera in ER+/HER2-, ER+/HER2 + and ER-/HER2 + tumors, while triple-negative samples exhibited a higher activity of Lactobacillus. In ER-negative samples (triple-negative and ER-/HER2+), 479 genes, including the breast carcinogenesis genes phospholipase A2, histone cluster 2, Crk-like, and cyclin D1, were significantly positive associated with the activity of Lactobacillus. Conclusion This was the first study to clarify an association between the breast tumor microbiota transcriptional activity and the expression of carcinogenesis genes in ER-negative breast cancer. Changes in the microbiota of breast tissue induced by external factors might be one of the key causes of ER negative breast cancer. |
| first_indexed | 2024-04-09T22:53:07Z |
| format | Article |
| id | doaj.art-a9cf5fc2041a44ceb7126a9c1ca51e79 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-09T22:53:07Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-a9cf5fc2041a44ceb7126a9c1ca51e792023-03-22T11:34:17ZengBMCBMC Cancer1471-24072023-03-0123111010.1186/s12885-023-10726-4Relationship between tumor microbiota transcriptional activity and gene expression in breast cancerEnuo Liu0Fan Zhang1Tiansheng Xu2Luyi Ye3Sean Si Qian Ma4Zai-Si Ji5NHC Key Laboratory of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan UniversityShanghai OE Biotech Co., LtdShanghai OE Biotech Co., LtdNHC Key Laboratory of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan UniversityNHC Key Laboratory of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan UniversityNHC Key Laboratory of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan UniversityAbstract Background A few studies have reported the distribution of the microbiota in breast cancer tissues, but few reports have compared the microbiota in different subtypes of breast cancer tissue. Moreover, no study has reported on the relationship between the microbiota and gene expression in breast tumor. Methods Sections of formalin-fixed paraffin-embedded (FFPE) tissue were prepared from the breast tumors of 70 patients and were subjected to microarray analysis to identify gene expression profiles. The same total RNA samples were also used to analyze the microbiota activity in tumor tissues by performing 16 S rRNA sequencing and internal transcribed spacer (ITS) sequencing of reverse transcript cDNA with Illumina Miseq. Pearson’s correlation coefficient was used for calculating the correlation between microbial relative activity and gene expression. Results The microbiota transcriptional activity of 70 FFPE samples mainly consisted of the phyla Bacteroidetes, Firmicutes and Proteobacteria. Prevotella_9, Bacteroides and Alloprevotella were the most active genera in ER+/HER2-, ER+/HER2 + and ER-/HER2 + tumors, while triple-negative samples exhibited a higher activity of Lactobacillus. In ER-negative samples (triple-negative and ER-/HER2+), 479 genes, including the breast carcinogenesis genes phospholipase A2, histone cluster 2, Crk-like, and cyclin D1, were significantly positive associated with the activity of Lactobacillus. Conclusion This was the first study to clarify an association between the breast tumor microbiota transcriptional activity and the expression of carcinogenesis genes in ER-negative breast cancer. Changes in the microbiota of breast tissue induced by external factors might be one of the key causes of ER negative breast cancer.https://doi.org/10.1186/s12885-023-10726-4Tumor microbiotaER-negative breast cancerCarcinogenesisLactobacillusBacteroides |
| spellingShingle | Enuo Liu Fan Zhang Tiansheng Xu Luyi Ye Sean Si Qian Ma Zai-Si Ji Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer BMC Cancer Tumor microbiota ER-negative breast cancer Carcinogenesis Lactobacillus Bacteroides |
| title | Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer |
| title_full | Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer |
| title_fullStr | Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer |
| title_full_unstemmed | Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer |
| title_short | Relationship between tumor microbiota transcriptional activity and gene expression in breast cancer |
| title_sort | relationship between tumor microbiota transcriptional activity and gene expression in breast cancer |
| topic | Tumor microbiota ER-negative breast cancer Carcinogenesis Lactobacillus Bacteroides |
| url | https://doi.org/10.1186/s12885-023-10726-4 |
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