Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain
Near-infrared (NIR) fluorescent imaging is a powerful tool for the non-invasive visualization of the inner structure of living organisms. Recently, NIR fluorescence imaging at 1000–1400 nm (second optical window) has been shown to offer better spatial resolution compared with conventional NIR fluore...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/21/8/1080 |
| _version_ | 1819206570492297216 |
|---|---|
| author | Yukio Imamura Sayumi Yamada Setsuko Tsuboi Yuko Nakane Yoshikazu Tsukasaki Akihito Komatsuzaki Takashi Jin |
| author_facet | Yukio Imamura Sayumi Yamada Setsuko Tsuboi Yuko Nakane Yoshikazu Tsukasaki Akihito Komatsuzaki Takashi Jin |
| author_sort | Yukio Imamura |
| collection | DOAJ |
| description | Near-infrared (NIR) fluorescent imaging is a powerful tool for the non-invasive visualization of the inner structure of living organisms. Recently, NIR fluorescence imaging at 1000–1400 nm (second optical window) has been shown to offer better spatial resolution compared with conventional NIR fluorescence imaging at 700–900 nm (first optical window). Here we report lead sulfide (PbS) quantum dots (QDs) and their use for in vivo NIR fluorescence imaging of cerebral venous thrombosis in septic mice. Highly fluorescent PbS QDs with a 1100 nm emission peak (QD1100) were prepared from lead acetate and hexamethyldisilathiane, and the surface of QD1100 was coated with mercaptoundecanoic acid so as to be soluble in water. NIR fluorescence imaging of the cerebral vessels of living mice was performed after intravascular injection (200–300 μL) of QD1100 (3 μM) from a caudal vein. By detecting the NIR fluorescence of QD1100, we achieved non-invasive NIR fluorescence imaging of cerebral blood vessels through the scalp and skull. We also achieved NIR fluorescence imaging of cerebral venous thrombosis in septic mice induced by the administration of lipopolysaccharide (LPS). From the NIR fluorescence imaging, we found that the number of thrombi in septic mice was significantly increased by the administration of LPS. The formation of thrombi in cerebral blood vessels in septic mice was confirmed by enzyme-linked immunosorbent assay (ELISA). We also found that the number of thrombi significantly decreased after the administration of heparin, an inhibitor of blood coagulation. These results show that NIR fluorescence imaging with QD1100 is useful for the evaluation of the pathological state of cerebral blood vessels in septic mice. |
| first_indexed | 2024-12-23T05:09:42Z |
| format | Article |
| id | doaj.art-a9cfa69c09214c5bbda584f2f6eab931 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T05:09:42Z |
| publishDate | 2016-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a9cfa69c09214c5bbda584f2f6eab9312022-12-21T17:59:01ZengMDPI AGMolecules1420-30492016-08-01218108010.3390/molecules21081080molecules21081080Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse BrainYukio Imamura0Sayumi Yamada1Setsuko Tsuboi2Yuko Nakane3Yoshikazu Tsukasaki4Akihito Komatsuzaki5Takashi Jin6Laboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanLaboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanLaboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanLaboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanLaboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanLaboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanLaboratory for Nano-Bio Probes, Quantitative Biology Center (QBiC), Riken, Suita 565-0874, JapanNear-infrared (NIR) fluorescent imaging is a powerful tool for the non-invasive visualization of the inner structure of living organisms. Recently, NIR fluorescence imaging at 1000–1400 nm (second optical window) has been shown to offer better spatial resolution compared with conventional NIR fluorescence imaging at 700–900 nm (first optical window). Here we report lead sulfide (PbS) quantum dots (QDs) and their use for in vivo NIR fluorescence imaging of cerebral venous thrombosis in septic mice. Highly fluorescent PbS QDs with a 1100 nm emission peak (QD1100) were prepared from lead acetate and hexamethyldisilathiane, and the surface of QD1100 was coated with mercaptoundecanoic acid so as to be soluble in water. NIR fluorescence imaging of the cerebral vessels of living mice was performed after intravascular injection (200–300 μL) of QD1100 (3 μM) from a caudal vein. By detecting the NIR fluorescence of QD1100, we achieved non-invasive NIR fluorescence imaging of cerebral blood vessels through the scalp and skull. We also achieved NIR fluorescence imaging of cerebral venous thrombosis in septic mice induced by the administration of lipopolysaccharide (LPS). From the NIR fluorescence imaging, we found that the number of thrombi in septic mice was significantly increased by the administration of LPS. The formation of thrombi in cerebral blood vessels in septic mice was confirmed by enzyme-linked immunosorbent assay (ELISA). We also found that the number of thrombi significantly decreased after the administration of heparin, an inhibitor of blood coagulation. These results show that NIR fluorescence imaging with QD1100 is useful for the evaluation of the pathological state of cerebral blood vessels in septic mice.http://www.mdpi.com/1420-3049/21/8/1080near-infrared fluorescencein vivo imagingquantum dotspathological statelipopolysaccharidesepsis |
| spellingShingle | Yukio Imamura Sayumi Yamada Setsuko Tsuboi Yuko Nakane Yoshikazu Tsukasaki Akihito Komatsuzaki Takashi Jin Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain Molecules near-infrared fluorescence in vivo imaging quantum dots pathological state lipopolysaccharide sepsis |
| title | Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain |
| title_full | Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain |
| title_fullStr | Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain |
| title_full_unstemmed | Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain |
| title_short | Near-Infrared Emitting PbS Quantum Dots for in Vivo Fluorescence Imaging of the Thrombotic State in Septic Mouse Brain |
| title_sort | near infrared emitting pbs quantum dots for in vivo fluorescence imaging of the thrombotic state in septic mouse brain |
| topic | near-infrared fluorescence in vivo imaging quantum dots pathological state lipopolysaccharide sepsis |
| url | http://www.mdpi.com/1420-3049/21/8/1080 |
| work_keys_str_mv | AT yukioimamura nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain AT sayumiyamada nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain AT setsukotsuboi nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain AT yukonakane nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain AT yoshikazutsukasaki nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain AT akihitokomatsuzaki nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain AT takashijin nearinfraredemittingpbsquantumdotsforinvivofluorescenceimagingofthethromboticstateinsepticmousebrain |