Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer
Background : Notch signaling played a critical role in promoting breast tumorigenesis and progression. However, the role and prognostic value of Notch3 combined with DLL4 expression in breast carcinoma had not been explored. Methods: The retrospective study enrolled 90 breast cancer tissues and 60 n...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-02-01
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| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338221118984 |
| _version_ | 1828040881385504768 |
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| author | Lin Wang Ge Cao Wei Li Xiao-Wen Chen Shi-Shuang Xiong Ya-Nan Mu Jin-Fang Jiang Lan Yang De-Rui Zhang Yu-Wen Cao |
| author_facet | Lin Wang Ge Cao Wei Li Xiao-Wen Chen Shi-Shuang Xiong Ya-Nan Mu Jin-Fang Jiang Lan Yang De-Rui Zhang Yu-Wen Cao |
| author_sort | Lin Wang |
| collection | DOAJ |
| description | Background : Notch signaling played a critical role in promoting breast tumorigenesis and progression. However, the role and prognostic value of Notch3 combined with DLL4 expression in breast carcinoma had not been explored. Methods: The retrospective study enrolled 90 breast cancer tissues and 60 noncancerous tissues from (conceal). The expression and prognostic value of Notch3 and DLL4 in patients with breast carcinoma were investigated using Oncomine and UALCAN database. Notch3 and DLL4 expression levels were detected by quantitative real-time polymerase chain reaction, western blotting, and immunohistochemistry. We analyzed the correlation between both proteins expression and clinicopathological parameters and survival data, respectively. Results: The expressions of Notch3 and DLL4 were increased, and Notch3 expression was significantly positively associated with DLL4 in breast carcinoma. The 2 proteins dramatically correlated with advanced stage, high grade and negative Her2 status. The overexpressing of single or both Notch3 and DLL4 resulted in shortened survival of breast cancer patients. And Notch3 overexpression was one of independent risk predictors to poor prognosis. Conclusion: The interaction of Notch3 receptor and DLL4 ligand accelerates oncogenesis, progression, and poor prognosis of breast cancer patients. Notch3 protein may serve as one of biomarker to independently predict prognosis of patients. |
| first_indexed | 2024-04-10T17:02:04Z |
| format | Article |
| id | doaj.art-a9d05523908d401ab7d7928eea601369 |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-04-10T17:02:04Z |
| publishDate | 2023-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-a9d05523908d401ab7d7928eea6013692023-02-06T10:03:47ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382023-02-012210.1177/15330338221118984Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast CancerLin Wang0Ge Cao1Wei Li2Xiao-Wen Chen3 Shi-Shuang Xiong4Ya-Nan Mu5Jin-Fang Jiang6Lan Yang7 De-Rui Zhang8Yu-Wen Cao9 Changle People's Hospital, Weifang, Shandong, China , Shihezi, Xinjiang, China Shandong Provincial Western Hospital, Jinan, , Shandong, China , Zhanjiang, Guangdong, China , Shihezi, Xinjiang, China , Shihezi, Xinjiang, China , Shihezi, Xinjiang, China , Shihezi, Xinjiang, China , Shihezi, Xinjiang, China The First Affiliated Hospital of Shihezi University School of Medicine, , Shihezi, Xinjiang, ChinaBackground : Notch signaling played a critical role in promoting breast tumorigenesis and progression. However, the role and prognostic value of Notch3 combined with DLL4 expression in breast carcinoma had not been explored. Methods: The retrospective study enrolled 90 breast cancer tissues and 60 noncancerous tissues from (conceal). The expression and prognostic value of Notch3 and DLL4 in patients with breast carcinoma were investigated using Oncomine and UALCAN database. Notch3 and DLL4 expression levels were detected by quantitative real-time polymerase chain reaction, western blotting, and immunohistochemistry. We analyzed the correlation between both proteins expression and clinicopathological parameters and survival data, respectively. Results: The expressions of Notch3 and DLL4 were increased, and Notch3 expression was significantly positively associated with DLL4 in breast carcinoma. The 2 proteins dramatically correlated with advanced stage, high grade and negative Her2 status. The overexpressing of single or both Notch3 and DLL4 resulted in shortened survival of breast cancer patients. And Notch3 overexpression was one of independent risk predictors to poor prognosis. Conclusion: The interaction of Notch3 receptor and DLL4 ligand accelerates oncogenesis, progression, and poor prognosis of breast cancer patients. Notch3 protein may serve as one of biomarker to independently predict prognosis of patients.https://doi.org/10.1177/15330338221118984 |
| spellingShingle | Lin Wang Ge Cao Wei Li Xiao-Wen Chen Shi-Shuang Xiong Ya-Nan Mu Jin-Fang Jiang Lan Yang De-Rui Zhang Yu-Wen Cao Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer Technology in Cancer Research & Treatment |
| title | Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer |
| title_full | Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer |
| title_fullStr | Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer |
| title_full_unstemmed | Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer |
| title_short | Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer |
| title_sort | expressions and prognostic values of notch3 and dll4 in human breast cancer |
| url | https://doi.org/10.1177/15330338221118984 |
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