IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds.
OBJECTIVE:Phosphatidylserine is exposed on apoptotic cells and is prone to oxidation (OxPS). Here we analyze the association of IgM antibodies against OxPS (anti-OxPS) with the risk of cardiovascular disease (CVD). METHODS:Among sixty-year olds from Stockholm County in Sweden, previously screened fo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5400230?pdf=render |
| _version_ | 1811235544759271424 |
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| author | Johan Frostegård Jun Su Sudhir Sing Xiang Hua Max Vikström Karin Leander Bruna Gigante Ulf de Faire Anna G Frostegård |
| author_facet | Johan Frostegård Jun Su Sudhir Sing Xiang Hua Max Vikström Karin Leander Bruna Gigante Ulf de Faire Anna G Frostegård |
| author_sort | Johan Frostegård |
| collection | DOAJ |
| description | OBJECTIVE:Phosphatidylserine is exposed on apoptotic cells and is prone to oxidation (OxPS). Here we analyze the association of IgM antibodies against OxPS (anti-OxPS) with the risk of cardiovascular disease (CVD). METHODS:Among sixty-year olds from Stockholm County in Sweden, previously screened for cardiovascular risk factors (2039 men, 2193 women), there were 210 incident CVD-cases identified during a 5-year follow-up. Using a nested case-control design, 622 age- and sex-matched controls were selected. Odds ratios (OR) with 95% intervals (CI) were calculated by conditional logistic regression. IgM anti-OxPS was measured by ELISA. Phagocytosis of apoptotic Jurkat-cells by macrophages was studied by flow cytometry. RESULTS:Anti-OxPS levels were lower among cases (median (interquartile range): 80.7 (60.9-101.0 vs. 84.6 (65.8-109.6); p = 0.047); among men (76.6 (55.8-99.2) vs. 82.0 (63.1-105.1); p = 0.022) and among women 89.6 (72.3-110.1) vs. 89.8 (69.9-114.4); p = 0.79). After adjustment for smoking, BMI, diabetes mellitus type II, hypercholesterolaemia and hypertension, and dividing into quartiles, using the highest quartile (quartile 4) as reference, quartile 3 was associated with a OR of 1.74 (CI 1.08-2.81). Quartiles 2 and 1 had similar associations, the later reaching statistical significance. Among men associations were stronger whereas no significant associations were observed in women. The OR of MI/angina comparing quartile 3 with quartile 4 was 2.31 (CI 1.30-4.11). The OR for quartile 2 and 1, respectively, were similar as for quartile 3. Total IgM increased uptake of apoptotic cells, which was reversed if incubated with OxPS. CONCLUSIONS:IgM anti-OxPS is a novel potential protection marker for CVD, in particular in men. Increased phagocytosis of dying/dead cells could be one potential underlying mechanism. |
| first_indexed | 2024-04-12T11:53:59Z |
| format | Article |
| id | doaj.art-a9d1e80ab00847d1a1260439fb26f7ab |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:53:59Z |
| publishDate | 2017-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-a9d1e80ab00847d1a1260439fb26f7ab2022-12-22T03:34:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017119510.1371/journal.pone.0171195IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds.Johan FrostegårdJun SuSudhir SingXiang HuaMax VikströmKarin LeanderBruna GiganteUlf de FaireAnna G FrostegårdOBJECTIVE:Phosphatidylserine is exposed on apoptotic cells and is prone to oxidation (OxPS). Here we analyze the association of IgM antibodies against OxPS (anti-OxPS) with the risk of cardiovascular disease (CVD). METHODS:Among sixty-year olds from Stockholm County in Sweden, previously screened for cardiovascular risk factors (2039 men, 2193 women), there were 210 incident CVD-cases identified during a 5-year follow-up. Using a nested case-control design, 622 age- and sex-matched controls were selected. Odds ratios (OR) with 95% intervals (CI) were calculated by conditional logistic regression. IgM anti-OxPS was measured by ELISA. Phagocytosis of apoptotic Jurkat-cells by macrophages was studied by flow cytometry. RESULTS:Anti-OxPS levels were lower among cases (median (interquartile range): 80.7 (60.9-101.0 vs. 84.6 (65.8-109.6); p = 0.047); among men (76.6 (55.8-99.2) vs. 82.0 (63.1-105.1); p = 0.022) and among women 89.6 (72.3-110.1) vs. 89.8 (69.9-114.4); p = 0.79). After adjustment for smoking, BMI, diabetes mellitus type II, hypercholesterolaemia and hypertension, and dividing into quartiles, using the highest quartile (quartile 4) as reference, quartile 3 was associated with a OR of 1.74 (CI 1.08-2.81). Quartiles 2 and 1 had similar associations, the later reaching statistical significance. Among men associations were stronger whereas no significant associations were observed in women. The OR of MI/angina comparing quartile 3 with quartile 4 was 2.31 (CI 1.30-4.11). The OR for quartile 2 and 1, respectively, were similar as for quartile 3. Total IgM increased uptake of apoptotic cells, which was reversed if incubated with OxPS. CONCLUSIONS:IgM anti-OxPS is a novel potential protection marker for CVD, in particular in men. Increased phagocytosis of dying/dead cells could be one potential underlying mechanism.http://europepmc.org/articles/PMC5400230?pdf=render |
| spellingShingle | Johan Frostegård Jun Su Sudhir Sing Xiang Hua Max Vikström Karin Leander Bruna Gigante Ulf de Faire Anna G Frostegård IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds. PLoS ONE |
| title | IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds. |
| title_full | IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds. |
| title_fullStr | IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds. |
| title_full_unstemmed | IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds. |
| title_short | IgM antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60-year olds. |
| title_sort | igm antibodies to oxidized phosphatidylserine as protection markers in cardiovascular disease among 60 year olds |
| url | http://europepmc.org/articles/PMC5400230?pdf=render |
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