Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts
This was an exploratory study designed to evaluate the feasibility of a recently established imaging modality, quantitative ultrashort time-to-echo contrast enhanced (QUTE-CE), to follow the early pathology and vulnerability of the blood brain barrier in response to single and repetitive mild head i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Neurology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2021.729464/full |
| _version_ | 1818920445708075008 |
|---|---|
| author | Joshua Leaston Ju Qiao Ian C. Harding Praveen Kulkarni Codi Gharagouzloo Codi Gharagouzloo Eno Ebong Craig F. Ferris Craig F. Ferris |
| author_facet | Joshua Leaston Ju Qiao Ian C. Harding Praveen Kulkarni Codi Gharagouzloo Codi Gharagouzloo Eno Ebong Craig F. Ferris Craig F. Ferris |
| author_sort | Joshua Leaston |
| collection | DOAJ |
| description | This was an exploratory study designed to evaluate the feasibility of a recently established imaging modality, quantitative ultrashort time-to-echo contrast enhanced (QUTE-CE), to follow the early pathology and vulnerability of the blood brain barrier in response to single and repetitive mild head impacts. A closed-head, momentum exchange model was used to produce three consecutive mild head impacts aimed at the forebrain separated by 24 h each. Animals were measured at baseline and within 1 h of impact. Anatomical images were collected to assess the extent of structural damage. QUTE-CE biomarkers for BBB permeability were calculated on 420,000 voxels in the brain and were registered to a bilateral 3D brain atlas providing site-specific information on 118 anatomical regions. Blood brain barrier permeability was confirmed by extravasation of labeled dextran. All head impacts occurred in the absence of any structural brain damage. A single mild head impact had measurable effects on blood brain barrier permeability and was more significant after the second and third impacts. Affected regions included the prefrontal ctx, basal ganglia, hippocampus, amygdala, and brainstem. Our findings support the concerns raised by the healthcare community regarding mild head injuries in participants in organized contact sports and military personnel in basic training and combat. |
| first_indexed | 2024-12-20T01:21:52Z |
| format | Article |
| id | doaj.art-a9d2372b7b8f44e2aaee36104f5a5663 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-20T01:21:52Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-a9d2372b7b8f44e2aaee36104f5a56632022-12-21T19:58:24ZengFrontiers Media S.A.Frontiers in Neurology1664-22952021-09-011210.3389/fneur.2021.729464729464Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head ImpactsJoshua Leaston0Ju Qiao1Ian C. Harding2Praveen Kulkarni3Codi Gharagouzloo4Codi Gharagouzloo5Eno Ebong6Craig F. Ferris7Craig F. Ferris8Imaginostics, Inc., Cambridge, MA, United StatesCenter for Translational Neuroimaging, Northeastern University, Boston, MA, United StatesDepartment of Bioengineering, Northeastern University, Boston, MA, United StatesImaginostics, Inc., Cambridge, MA, United StatesImaginostics, Inc., Cambridge, MA, United StatesCenter for Translational Neuroimaging, Northeastern University, Boston, MA, United StatesDepartment of Chemical Engineering, Northeastern University, Boston, MA, United StatesCenter for Translational Neuroimaging, Northeastern University, Boston, MA, United StatesDepartments of Psychology and Pharmaceutical Sciences, Northeastern University, Boston, MA, United StatesThis was an exploratory study designed to evaluate the feasibility of a recently established imaging modality, quantitative ultrashort time-to-echo contrast enhanced (QUTE-CE), to follow the early pathology and vulnerability of the blood brain barrier in response to single and repetitive mild head impacts. A closed-head, momentum exchange model was used to produce three consecutive mild head impacts aimed at the forebrain separated by 24 h each. Animals were measured at baseline and within 1 h of impact. Anatomical images were collected to assess the extent of structural damage. QUTE-CE biomarkers for BBB permeability were calculated on 420,000 voxels in the brain and were registered to a bilateral 3D brain atlas providing site-specific information on 118 anatomical regions. Blood brain barrier permeability was confirmed by extravasation of labeled dextran. All head impacts occurred in the absence of any structural brain damage. A single mild head impact had measurable effects on blood brain barrier permeability and was more significant after the second and third impacts. Affected regions included the prefrontal ctx, basal ganglia, hippocampus, amygdala, and brainstem. Our findings support the concerns raised by the healthcare community regarding mild head injuries in participants in organized contact sports and military personnel in basic training and combat.https://www.frontiersin.org/articles/10.3389/fneur.2021.729464/fullconcussionferumoxytolcerebral small vessel diseasemomentum exchangequantitative ultrashort time-to-echocontrast enhanced MRI |
| spellingShingle | Joshua Leaston Ju Qiao Ian C. Harding Praveen Kulkarni Codi Gharagouzloo Codi Gharagouzloo Eno Ebong Craig F. Ferris Craig F. Ferris Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts Frontiers in Neurology concussion ferumoxytol cerebral small vessel disease momentum exchange quantitative ultrashort time-to-echo contrast enhanced MRI |
| title | Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts |
| title_full | Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts |
| title_fullStr | Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts |
| title_full_unstemmed | Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts |
| title_short | Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts |
| title_sort | quantitative imaging of blood brain barrier permeability following repetitive mild head impacts |
| topic | concussion ferumoxytol cerebral small vessel disease momentum exchange quantitative ultrashort time-to-echo contrast enhanced MRI |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2021.729464/full |
| work_keys_str_mv | AT joshualeaston quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT juqiao quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT iancharding quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT praveenkulkarni quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT codigharagouzloo quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT codigharagouzloo quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT enoebong quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT craigfferris quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts AT craigfferris quantitativeimagingofbloodbrainbarrierpermeabilityfollowingrepetitivemildheadimpacts |