Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway
Abstract Background Cancer pain is a debilitating disorder of human breast cancer and a primary determinant of the poor quality of life, and relieving pain is fundamental strategy in the cancer treatment. However, opioid analgesics, like morphine and fentanyl, which are widely used in cancer pain tr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-05-01
|
| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13046-019-1184-1 |
| _version_ | 1818027955099533312 |
|---|---|
| author | Jiachuan Yu Yuanyuan Luo Qingping Wen |
| author_facet | Jiachuan Yu Yuanyuan Luo Qingping Wen |
| author_sort | Jiachuan Yu |
| collection | DOAJ |
| description | Abstract Background Cancer pain is a debilitating disorder of human breast cancer and a primary determinant of the poor quality of life, and relieving pain is fundamental strategy in the cancer treatment. However, opioid analgesics, like morphine and fentanyl, which are widely used in cancer pain treatment have been reported to enhance stem-like traits and epithelial-mesenchymal transition (EMT) of breast cancer cells. As such, it is vital to make the best choice of analgesic for breast cancer management. Methods MTT assays and colony formation assays were performed to examine tumor cell proliferation upon nalbuphine treatment. RT-PCR, western blot, flow cytometry, sphere formation, immunohistochemistry, transwell assays, wound healing assays and mouse xenograft were used to assess the biological effects of nalbuphine treatment. Results Nalbuphine inhibited breast cancer cell growth and tumorigenesis, with little effect on noncancerous breast cell lines. Nalbuphine suppressed cancer stem-like traits and EMT in both breast cancer cells and mouse xenograft tumor tissues. Additionally, activation of AKT reversed the nalbuphine-induced inhibition of cancer stem-like properties, tumorigenesis and EMT. Conclusions Our results demonstrate a new role of nalbuphine in inhibiting cancer stem-like properties and EMT in addition to relieving pain, which suggests that nalbuphine may be effective in breast cancer treatment. |
| first_indexed | 2024-12-10T04:56:07Z |
| format | Article |
| id | doaj.art-a9d477bebfde4c69b58bc0a2463d078b |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-10T04:56:07Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-a9d477bebfde4c69b58bc0a2463d078b2022-12-22T02:01:30ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662019-05-0138111010.1186/s13046-019-1184-1Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathwayJiachuan Yu0Yuanyuan Luo1Qingping Wen2Department of Anesthesiology, The First Affiliated Hospital of Dalian Medical UniversityInstitute of Cancer Stem Cell, Dalian Medical UniversityDepartment of Anesthesiology, The First Affiliated Hospital of Dalian Medical UniversityAbstract Background Cancer pain is a debilitating disorder of human breast cancer and a primary determinant of the poor quality of life, and relieving pain is fundamental strategy in the cancer treatment. However, opioid analgesics, like morphine and fentanyl, which are widely used in cancer pain treatment have been reported to enhance stem-like traits and epithelial-mesenchymal transition (EMT) of breast cancer cells. As such, it is vital to make the best choice of analgesic for breast cancer management. Methods MTT assays and colony formation assays were performed to examine tumor cell proliferation upon nalbuphine treatment. RT-PCR, western blot, flow cytometry, sphere formation, immunohistochemistry, transwell assays, wound healing assays and mouse xenograft were used to assess the biological effects of nalbuphine treatment. Results Nalbuphine inhibited breast cancer cell growth and tumorigenesis, with little effect on noncancerous breast cell lines. Nalbuphine suppressed cancer stem-like traits and EMT in both breast cancer cells and mouse xenograft tumor tissues. Additionally, activation of AKT reversed the nalbuphine-induced inhibition of cancer stem-like properties, tumorigenesis and EMT. Conclusions Our results demonstrate a new role of nalbuphine in inhibiting cancer stem-like properties and EMT in addition to relieving pain, which suggests that nalbuphine may be effective in breast cancer treatment.http://link.springer.com/article/10.1186/s13046-019-1184-1NalbuphineBreast cancerStem-like traitsEpithelial-mesenchymal transitionAKT-NFκB pathway |
| spellingShingle | Jiachuan Yu Yuanyuan Luo Qingping Wen Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway Journal of Experimental & Clinical Cancer Research Nalbuphine Breast cancer Stem-like traits Epithelial-mesenchymal transition AKT-NFκB pathway |
| title | Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway |
| title_full | Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway |
| title_fullStr | Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway |
| title_full_unstemmed | Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway |
| title_short | Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway |
| title_sort | nalbuphine suppresses breast cancer stem like properties and epithelial mesenchymal transition via the akt nfκb signaling pathway |
| topic | Nalbuphine Breast cancer Stem-like traits Epithelial-mesenchymal transition AKT-NFκB pathway |
| url | http://link.springer.com/article/10.1186/s13046-019-1184-1 |
| work_keys_str_mv | AT jiachuanyu nalbuphinesuppressesbreastcancerstemlikepropertiesandepithelialmesenchymaltransitionviatheaktnfkbsignalingpathway AT yuanyuanluo nalbuphinesuppressesbreastcancerstemlikepropertiesandepithelialmesenchymaltransitionviatheaktnfkbsignalingpathway AT qingpingwen nalbuphinesuppressesbreastcancerstemlikepropertiesandepithelialmesenchymaltransitionviatheaktnfkbsignalingpathway |