Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung
Typical and atypical carcinoid tumors belong to the neuroendocrine lung tumors. They have low recurrence and proliferation rate, lymph node, and distant metastases. Nevertheless, these tumors have shown a more aggressive behavior. In the last years, microRNAs were screened as new tumor markers for t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOS Press
2017-10-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317728417 |
| _version_ | 1818574846201692160 |
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| author | Pietro Di Fazio Moritz Maass Silvia Roth Christian Meyer Joana Grups Peter Rexin Detlef K Bartsch Andreas Kirschbaum |
| author_facet | Pietro Di Fazio Moritz Maass Silvia Roth Christian Meyer Joana Grups Peter Rexin Detlef K Bartsch Andreas Kirschbaum |
| author_sort | Pietro Di Fazio |
| collection | DOAJ |
| description | Typical and atypical carcinoid tumors belong to the neuroendocrine lung tumors. They have low recurrence and proliferation rate, lymph node, and distant metastases. Nevertheless, these tumors have shown a more aggressive behavior. In the last years, microRNAs were screened as new tumor markers for their potential diagnostic and therapeutic relevance. The expression of hsa-let-7b-5p, hsa-let-7f-5p, hsa-miR-222-3p, and their targets HMGA2 (high-mobility group A2) and CDKN1B (cyclin-dependent kynase inhibitor 1B, p27 kip1 ) was evaluated in this rare small group of patients. We analyzed the clinical data of all typical and atypical carcinoid tumors of patients who underwent surgical operation at Marburg University Hospital (n = 18) from 2000. Quantitative reverse transcription polymerase chain reaction was performed in formalin-fixed paraffin-embedded tumor tissue versus four tumor-free lung tissue samples. HMGA2 was stable or downregulated; only one patient showed a significant overexpression. CDKN1B showed a significant overexpression or a stable level; it was downregulated in two samples only. Hsa-miR-222-3p resulted almost stable or overexpressed except for two samples (significantly downregulated). Hsa-let-7f-5p was stable or overexpressed in the majority of analyzed samples, whereas hsa-let-7b-5p was significantly downregulated. HMGA2 and CDKN1B are differently expressed between atypical and typical carcinoid tumors, thus representing valid biomarkers for the classification of the two tumor groups. Hsa-let-7f-5p and HMGA2 are inversely correlated. Hsa-miR-222-3p does not correlate with its predicted target CDKN1B. |
| first_indexed | 2024-12-15T00:31:31Z |
| format | Article |
| id | doaj.art-a9d4cc05fce04211b9384d25f170e165 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-15T00:31:31Z |
| publishDate | 2017-10-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-a9d4cc05fce04211b9384d25f170e1652022-12-21T22:41:59ZengIOS PressTumor Biology1423-03802017-10-013910.1177/1010428317728417Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lungPietro Di Fazio0Moritz Maass1Silvia Roth2Christian Meyer3Joana Grups4Peter Rexin5Detlef K Bartsch6Andreas Kirschbaum7Department of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyDepartment of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyDepartment of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyDepartment of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyDepartment of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyInstitute for Pathology, Philipps University of Marburg, Marburg, GermanyDepartment of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyDepartment of Visceral, Thoracic and Vascular Surgery, Philipps University of Marburg, Marburg, GermanyTypical and atypical carcinoid tumors belong to the neuroendocrine lung tumors. They have low recurrence and proliferation rate, lymph node, and distant metastases. Nevertheless, these tumors have shown a more aggressive behavior. In the last years, microRNAs were screened as new tumor markers for their potential diagnostic and therapeutic relevance. The expression of hsa-let-7b-5p, hsa-let-7f-5p, hsa-miR-222-3p, and their targets HMGA2 (high-mobility group A2) and CDKN1B (cyclin-dependent kynase inhibitor 1B, p27 kip1 ) was evaluated in this rare small group of patients. We analyzed the clinical data of all typical and atypical carcinoid tumors of patients who underwent surgical operation at Marburg University Hospital (n = 18) from 2000. Quantitative reverse transcription polymerase chain reaction was performed in formalin-fixed paraffin-embedded tumor tissue versus four tumor-free lung tissue samples. HMGA2 was stable or downregulated; only one patient showed a significant overexpression. CDKN1B showed a significant overexpression or a stable level; it was downregulated in two samples only. Hsa-miR-222-3p resulted almost stable or overexpressed except for two samples (significantly downregulated). Hsa-let-7f-5p was stable or overexpressed in the majority of analyzed samples, whereas hsa-let-7b-5p was significantly downregulated. HMGA2 and CDKN1B are differently expressed between atypical and typical carcinoid tumors, thus representing valid biomarkers for the classification of the two tumor groups. Hsa-let-7f-5p and HMGA2 are inversely correlated. Hsa-miR-222-3p does not correlate with its predicted target CDKN1B.https://doi.org/10.1177/1010428317728417 |
| spellingShingle | Pietro Di Fazio Moritz Maass Silvia Roth Christian Meyer Joana Grups Peter Rexin Detlef K Bartsch Andreas Kirschbaum Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung Tumor Biology |
| title | Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung |
| title_full | Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung |
| title_fullStr | Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung |
| title_full_unstemmed | Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung |
| title_short | Expression of hsa-let-7b-5p, hsa-let-7f-5p, and hsa-miR-222-3p and their putative targets HMGA2 and CDKN1B in typical and atypical carcinoid tumors of the lung |
| title_sort | expression of hsa let 7b 5p hsa let 7f 5p and hsa mir 222 3p and their putative targets hmga2 and cdkn1b in typical and atypical carcinoid tumors of the lung |
| url | https://doi.org/10.1177/1010428317728417 |
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