Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV
Respiratory syncytial virus (RSV) is one of the leading causes of lower respiratory tract infection in infants. Immunoprophylaxis with the anti-RSV monoclonal antibody, palivizumab, reduces the risk for RSV-related hospitalizations, but its use is restricted to high-risk infants due to the high cost...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Vaccines |
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| Online Access: | http://www.mdpi.com/2076-393X/7/1/3 |
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| author | Rika Gomi Anurag Sharma Wenzhu Wu Stefan Worgall |
| author_facet | Rika Gomi Anurag Sharma Wenzhu Wu Stefan Worgall |
| author_sort | Rika Gomi |
| collection | DOAJ |
| description | Respiratory syncytial virus (RSV) is one of the leading causes of lower respiratory tract infection in infants. Immunoprophylaxis with the anti-RSV monoclonal antibody, palivizumab, reduces the risk for RSV-related hospitalizations, but its use is restricted to high-risk infants due to the high costs. In this study, we investigated if genetic delivery of anti-RSV antibody to neonatal mice by chimpanzee adenovirus type 7 expressing the murine form of palivizumab (AdC7αRSV) can provide protection against RSV. Intranasal and intramuscular administration of AdC7αRSV to adult mice resulted in similar levels of anti-RSV IgG in the serum. However, only intranasal administration resulted in detectable levels of anti-RSV IgG in the bronchoalveolar lavage fluid. Intranasal administration of AdC7αRSV provided protection against subsequent RSV challenge. Expression of the anti-RSV antibody was prolonged following intranasal administration of AdC7αRSV to neonatal mice. Protection against RSV was confirmed at 6 weeks of age. These data suggest that neonatal genetic delivery of anti-RSV antibody by AdC7αRSV can provide protection against RSV. |
| first_indexed | 2024-04-12T05:37:55Z |
| format | Article |
| id | doaj.art-a9e37bb441fa46fd98f4100040b579dc |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-12T05:37:55Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-a9e37bb441fa46fd98f4100040b579dc2022-12-22T03:45:46ZengMDPI AGVaccines2076-393X2018-12-0171310.3390/vaccines7010003vaccines7010003Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSVRika Gomi0Anurag Sharma1Wenzhu Wu2Stefan Worgall3Department of Pediatrics, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pediatrics, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pediatrics, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pediatrics, Weill Cornell Medicine, New York, NY 10065, USARespiratory syncytial virus (RSV) is one of the leading causes of lower respiratory tract infection in infants. Immunoprophylaxis with the anti-RSV monoclonal antibody, palivizumab, reduces the risk for RSV-related hospitalizations, but its use is restricted to high-risk infants due to the high costs. In this study, we investigated if genetic delivery of anti-RSV antibody to neonatal mice by chimpanzee adenovirus type 7 expressing the murine form of palivizumab (AdC7αRSV) can provide protection against RSV. Intranasal and intramuscular administration of AdC7αRSV to adult mice resulted in similar levels of anti-RSV IgG in the serum. However, only intranasal administration resulted in detectable levels of anti-RSV IgG in the bronchoalveolar lavage fluid. Intranasal administration of AdC7αRSV provided protection against subsequent RSV challenge. Expression of the anti-RSV antibody was prolonged following intranasal administration of AdC7αRSV to neonatal mice. Protection against RSV was confirmed at 6 weeks of age. These data suggest that neonatal genetic delivery of anti-RSV antibody by AdC7αRSV can provide protection against RSV.http://www.mdpi.com/2076-393X/7/1/3respiratory syncytial virus (RSV)immunoprophylaxispalivizumabchimpanzee adenovirus type 7 (AdC7)neonatepassive immunizationgenetic gene delivery |
| spellingShingle | Rika Gomi Anurag Sharma Wenzhu Wu Stefan Worgall Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV Vaccines respiratory syncytial virus (RSV) immunoprophylaxis palivizumab chimpanzee adenovirus type 7 (AdC7) neonate passive immunization genetic gene delivery |
| title | Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV |
| title_full | Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV |
| title_fullStr | Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV |
| title_full_unstemmed | Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV |
| title_short | Neonatal Genetic Delivery of Anti-Respiratory Syncytial Virus (RSV) Antibody by Non-Human Primate-Based Adenoviral Vector to Provide Protection against RSV |
| title_sort | neonatal genetic delivery of anti respiratory syncytial virus rsv antibody by non human primate based adenoviral vector to provide protection against rsv |
| topic | respiratory syncytial virus (RSV) immunoprophylaxis palivizumab chimpanzee adenovirus type 7 (AdC7) neonate passive immunization genetic gene delivery |
| url | http://www.mdpi.com/2076-393X/7/1/3 |
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