Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
In the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, c...
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MDPI AG
2020-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/1/30 |
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| author | Sung Hyun Noh Han-Saem Jo Somang Choi Hee Gyeong Song Hak-Jun Kim Keung Nyun Kim Sung Eun Kim Kyeongsoon Park |
| author_facet | Sung Hyun Noh Han-Saem Jo Somang Choi Hee Gyeong Song Hak-Jun Kim Keung Nyun Kim Sung Eun Kim Kyeongsoon Park |
| author_sort | Sung Hyun Noh |
| collection | DOAJ |
| description | In the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, calcium accumulation, and expression of osteo-differentiation-specific genes, including osteocalcin (<i>OCN</i>) and osteopontin (<i>OPN</i>). Both bare MSNs and LF/TA-MSNs exhibited round nano-particle structures. The LF/TA-MSNs demonstrated prolonged LF release for up to 28 days. Treatment of ADSCs with LF (50 μg)/TA-MSNs resulted in markedly higher ALP level and calcium accumulation compared to treatment with LF (10 μg)/TA-MSNs or bare MSNs. Furthermore, LF (50 μg)/TA-MSNs remarkably increased mRNA levels of osteo-differentiation-specific genes, including <i>OCN</i> and <i>OPN</i>, compared to MSNs or LF (10 μg)/TA-MSNs. Together, these data suggest that the ability of LF/TA-MSNs to enhance osteo-differentiation of ADSCs make them a possible nanovehicle for bone healing and bone regeneration in patients with bone defect or disease. |
| first_indexed | 2024-03-10T13:45:46Z |
| format | Article |
| id | doaj.art-a9e4991b2cfb414ca26689c825724fd8 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T13:45:46Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-a9e4991b2cfb414ca26689c825724fd82023-11-21T02:39:35ZengMDPI AGPharmaceutics1999-49232020-12-011313010.3390/pharmaceutics13010030Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation AbilitySung Hyun Noh0Han-Saem Jo1Somang Choi2Hee Gyeong Song3Hak-Jun Kim4Keung Nyun Kim5Sung Eun Kim6Kyeongsoon Park7Department of Neurosurgery, National Health Insurance Service Ilsan Hospital, #100, Ilsan-ro, Ilsan-donggu, Gyeonggi-do, Goyang-si 10444, KoreaDepartment of Systems Biotechnology, Chung-Ang University, Gyeonggi-do, Anseong-si 17546, KoreaDepartment of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, KoreaDepartment of Systems Biotechnology, Chung-Ang University, Gyeonggi-do, Anseong-si 17546, KoreaDepartment of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, KoreaDepartment of Neurosurgery, Spine and Spinal Cord Institute, Severance Hospital, Yonsei University College of Medicine, #50, Yonsei-ro, Seodaemun-gu, Seoul 03722, KoreaDepartment of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, KoreaDepartment of Systems Biotechnology, Chung-Ang University, Gyeonggi-do, Anseong-si 17546, KoreaIn the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, calcium accumulation, and expression of osteo-differentiation-specific genes, including osteocalcin (<i>OCN</i>) and osteopontin (<i>OPN</i>). Both bare MSNs and LF/TA-MSNs exhibited round nano-particle structures. The LF/TA-MSNs demonstrated prolonged LF release for up to 28 days. Treatment of ADSCs with LF (50 μg)/TA-MSNs resulted in markedly higher ALP level and calcium accumulation compared to treatment with LF (10 μg)/TA-MSNs or bare MSNs. Furthermore, LF (50 μg)/TA-MSNs remarkably increased mRNA levels of osteo-differentiation-specific genes, including <i>OCN</i> and <i>OPN</i>, compared to MSNs or LF (10 μg)/TA-MSNs. Together, these data suggest that the ability of LF/TA-MSNs to enhance osteo-differentiation of ADSCs make them a possible nanovehicle for bone healing and bone regeneration in patients with bone defect or disease.https://www.mdpi.com/1999-4923/13/1/30mesoporous silica nanomaterialslactoferrinadipose-derived stem cellsosteo-differentiation ability |
| spellingShingle | Sung Hyun Noh Han-Saem Jo Somang Choi Hee Gyeong Song Hak-Jun Kim Keung Nyun Kim Sung Eun Kim Kyeongsoon Park Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability Pharmaceutics mesoporous silica nanomaterials lactoferrin adipose-derived stem cells osteo-differentiation ability |
| title | Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability |
| title_full | Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability |
| title_fullStr | Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability |
| title_full_unstemmed | Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability |
| title_short | Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability |
| title_sort | lactoferrin anchored tannylated mesoporous silica nanomaterials for enhanced osteo differentiation ability |
| topic | mesoporous silica nanomaterials lactoferrin adipose-derived stem cells osteo-differentiation ability |
| url | https://www.mdpi.com/1999-4923/13/1/30 |
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