Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples

Performance of an immunoenzymatic assay for Cryptosporidium diagnosis of fecal samples

We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen sta...

Full description

Bibliographic Details
Main Authors: Fabiana Rangel Marques, Luciana Ventura Cardoso, Carlos Eugênio Cavasini, Magali Carmem de Almeida, Nair Aparecida Bassi, Margarete Teresa Gottardo de Almeida, Andréa Regina Baptista Rossit, Ricardo Luiz Dantas Machado
Format: Article
Language:English
Published: Elsevier
Series:Brazilian Journal of Infectious Diseases
Subjects:
Parasites
acquired immunodeficiency syndrome
Cryptosporidium diagnosis
Brazil
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100002&lng=en&tlng=en
Description
Summary:We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.
ISSN:1678-4391

Similar Items