Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains.
C57BL/6J (B6J) and C57BL/6N (B6N) mice are the most frequently used substrains in C57BL/6 (B6) inbred mice, serving as physiological models for in vivo studies and as background strains to build transgenic mice. However, the differences in metabolic phenotypes between B6J and B6N mice are not cohere...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0271651 |
| _version_ | 1828067316588347392 |
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| author | Shino Nemoto Tetsuya Kubota Hiroshi Ohno |
| author_facet | Shino Nemoto Tetsuya Kubota Hiroshi Ohno |
| author_sort | Shino Nemoto |
| collection | DOAJ |
| description | C57BL/6J (B6J) and C57BL/6N (B6N) mice are the most frequently used substrains in C57BL/6 (B6) inbred mice, serving as physiological models for in vivo studies and as background strains to build transgenic mice. However, the differences in metabolic phenotypes between B6J and B6N mice are not coherent, and genotypic differences in metabolically important tissues have not been well studied. The phenotypic differences between B6J and B6N substrains have often been attributed to the role of the nicotinamide nucleotide transhydrogenase (Nnt) gene, whereby B6J has a spontaneous missense mutation of Nnt. Nevertheless, phenotypic differences between the two cannot be explained by Nnt mutations alone, especially in metabolic traits. Therefore, we aimed to investigate the genetic cause of the phenotypic differences between B6J and B6N mice. Determining consistent genetic differences across multiple tissues involved in metabolic traits such as subcutaneous and visceral white adipose tissues, brown adipose tissue, skeletal muscle, liver, hypothalamus, and hippocampus, may help explain phenotypic differences in metabolism between the two substrains. We report candidate genes along with comparative data on body weight, tissue weight, blood components involved in metabolism, and energy balance of B6J and B6N mice. Insulin degrading enzyme, adenylosuccinate synthase 2, and ectonucleotide triphosphate diphosphohydrolase 4 were highly expressed in B6J mice compared with those in B6N mice, and Nnt, WD repeat and FYVE domain containing 1, and dynein light chain Tctex-type 1 were less expressed in B6J mice compared with those in B6N mice in all seven tissues. Considering the extremely wide use of both substrains and their critical importance in generating transgenic and knock-out models, these findings guide future research across several interrelated fields. |
| first_indexed | 2024-04-10T23:45:02Z |
| format | Article |
| id | doaj.art-a9e67281dbf0466ca1f80fc7836ed433 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T23:45:02Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a9e67281dbf0466ca1f80fc7836ed4332023-01-11T05:31:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712e027165110.1371/journal.pone.0271651Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains.Shino NemotoTetsuya KubotaHiroshi OhnoC57BL/6J (B6J) and C57BL/6N (B6N) mice are the most frequently used substrains in C57BL/6 (B6) inbred mice, serving as physiological models for in vivo studies and as background strains to build transgenic mice. However, the differences in metabolic phenotypes between B6J and B6N mice are not coherent, and genotypic differences in metabolically important tissues have not been well studied. The phenotypic differences between B6J and B6N substrains have often been attributed to the role of the nicotinamide nucleotide transhydrogenase (Nnt) gene, whereby B6J has a spontaneous missense mutation of Nnt. Nevertheless, phenotypic differences between the two cannot be explained by Nnt mutations alone, especially in metabolic traits. Therefore, we aimed to investigate the genetic cause of the phenotypic differences between B6J and B6N mice. Determining consistent genetic differences across multiple tissues involved in metabolic traits such as subcutaneous and visceral white adipose tissues, brown adipose tissue, skeletal muscle, liver, hypothalamus, and hippocampus, may help explain phenotypic differences in metabolism between the two substrains. We report candidate genes along with comparative data on body weight, tissue weight, blood components involved in metabolism, and energy balance of B6J and B6N mice. Insulin degrading enzyme, adenylosuccinate synthase 2, and ectonucleotide triphosphate diphosphohydrolase 4 were highly expressed in B6J mice compared with those in B6N mice, and Nnt, WD repeat and FYVE domain containing 1, and dynein light chain Tctex-type 1 were less expressed in B6J mice compared with those in B6N mice in all seven tissues. Considering the extremely wide use of both substrains and their critical importance in generating transgenic and knock-out models, these findings guide future research across several interrelated fields.https://doi.org/10.1371/journal.pone.0271651 |
| spellingShingle | Shino Nemoto Tetsuya Kubota Hiroshi Ohno Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains. PLoS ONE |
| title | Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains. |
| title_full | Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains. |
| title_fullStr | Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains. |
| title_full_unstemmed | Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains. |
| title_short | Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains. |
| title_sort | metabolic differences and differentially expressed genes between c57bl 6j and c57bl 6n mice substrains |
| url | https://doi.org/10.1371/journal.pone.0271651 |
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