MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury
Our previous research studies have demonstrated the role of microRNA133b (miR133b) in healing the contused spinal cord when administered either intranasally or intravenously 24 h following an injury. While our data showed beneficial effects of exogenous miR133b delivered within hours of a spinal cor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/3058 |
| _version_ | 1797264384273154048 |
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| author | James Young Ho Yu Thomas C. Chen Camelia A. Danilov |
| author_facet | James Young Ho Yu Thomas C. Chen Camelia A. Danilov |
| author_sort | James Young Ho Yu |
| collection | DOAJ |
| description | Our previous research studies have demonstrated the role of microRNA133b (miR133b) in healing the contused spinal cord when administered either intranasally or intravenously 24 h following an injury. While our data showed beneficial effects of exogenous miR133b delivered within hours of a spinal cord injury (SCI), the kinetics of endogenous miR133b levels in the contused spinal cord and rostral/caudal segments of the injury were not fully investigated. In this study, we examined the miR133b dysregulation in a mouse model of moderate unilateral contusion injury at the fifth cervical (C5) level. Between 30 min and 7 days post-injury, mice were euthanized and tissues were collected from different areas of the spinal cord, ipsilateral and contralateral prefrontal motor cortices, and off-targets such as lung and spleen. The endogenous level of miR133b was determined by RT-qPCR. We found that after SCI, (a) most changes in miR133b level were restricted to the injured area with very limited alterations in the rostral and caudal parts relative to the injury site, (b) acute changes in the endogenous levels were predominantly specific to the lesion site with delayed miR133b changes in the motor cortex, and (c) ipsilateral and contralateral hemispheres responded differently to unilateral SCI. Our results suggest that the therapeutic window for exogenous miR133b therapy begins earlier than 24 h post-injury and potentially lasts longer than 7 days. |
| first_indexed | 2024-04-25T00:28:03Z |
| format | Article |
| id | doaj.art-a9e81c16774e4bbbb1b3bc577c1b6319 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:28:03Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a9e81c16774e4bbbb1b3bc577c1b63192024-03-12T16:47:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255305810.3390/ijms25053058MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion InjuryJames Young Ho Yu0Thomas C. Chen1Camelia A. Danilov2Department of Neurological Surgery, University of Southern California, 1200 N State St., Suite 3300, Los Angeles, CA 90033, USADepartment of Neurological Surgery, University of Southern California, 1200 N State St., Suite 3300, Los Angeles, CA 90033, USADepartment of Neurological Surgery, University of Southern California, 2011 Zonal Ave., Los Angeles, CA 90089, USAOur previous research studies have demonstrated the role of microRNA133b (miR133b) in healing the contused spinal cord when administered either intranasally or intravenously 24 h following an injury. While our data showed beneficial effects of exogenous miR133b delivered within hours of a spinal cord injury (SCI), the kinetics of endogenous miR133b levels in the contused spinal cord and rostral/caudal segments of the injury were not fully investigated. In this study, we examined the miR133b dysregulation in a mouse model of moderate unilateral contusion injury at the fifth cervical (C5) level. Between 30 min and 7 days post-injury, mice were euthanized and tissues were collected from different areas of the spinal cord, ipsilateral and contralateral prefrontal motor cortices, and off-targets such as lung and spleen. The endogenous level of miR133b was determined by RT-qPCR. We found that after SCI, (a) most changes in miR133b level were restricted to the injured area with very limited alterations in the rostral and caudal parts relative to the injury site, (b) acute changes in the endogenous levels were predominantly specific to the lesion site with delayed miR133b changes in the motor cortex, and (c) ipsilateral and contralateral hemispheres responded differently to unilateral SCI. Our results suggest that the therapeutic window for exogenous miR133b therapy begins earlier than 24 h post-injury and potentially lasts longer than 7 days.https://www.mdpi.com/1422-0067/25/5/3058microRNA133bspinal cord injuryspine traumascar tissuecervical spinemotor cortex |
| spellingShingle | James Young Ho Yu Thomas C. Chen Camelia A. Danilov MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury International Journal of Molecular Sciences microRNA133b spinal cord injury spine trauma scar tissue cervical spine motor cortex |
| title | MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury |
| title_full | MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury |
| title_fullStr | MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury |
| title_full_unstemmed | MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury |
| title_short | MicroRNA-133b Dysregulation in a Mouse Model of Cervical Contusion Injury |
| title_sort | microrna 133b dysregulation in a mouse model of cervical contusion injury |
| topic | microRNA133b spinal cord injury spine trauma scar tissue cervical spine motor cortex |
| url | https://www.mdpi.com/1422-0067/25/5/3058 |
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