Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response
Both strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4+ T cells and macrophages have been identified as major effector cells...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.917389/full |
| _version_ | 1818256197484019712 |
|---|---|
| author | Amarin Rittipornlertrak Amarin Rittipornlertrak Boondarika Nambooppha Boondarika Nambooppha Anucha Muenthaisong Nisachon Apinda Pongpisid Koonyosying Wanwisa Srisawat Paweena Chomjit Kanokwan Sangkakam Veerasak Punyapornwithaya Saruda Tiwananthagorn Naoaki Yokoyama Nattawooti Sthitmatee Nattawooti Sthitmatee Nattawooti Sthitmatee |
| author_facet | Amarin Rittipornlertrak Amarin Rittipornlertrak Boondarika Nambooppha Boondarika Nambooppha Anucha Muenthaisong Nisachon Apinda Pongpisid Koonyosying Wanwisa Srisawat Paweena Chomjit Kanokwan Sangkakam Veerasak Punyapornwithaya Saruda Tiwananthagorn Naoaki Yokoyama Nattawooti Sthitmatee Nattawooti Sthitmatee Nattawooti Sthitmatee |
| author_sort | Amarin Rittipornlertrak |
| collection | DOAJ |
| description | Both strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4+ T cells and macrophages have been identified as major effector cells mediating the clearance of pathogens. Previously, the apical membrane antigen 1 (AMA-1) was found to significantly induce the immune response inhibiting B. bovis merozoite growth and invasion. However, a detailed characterization of both humoral and cellular immune responses against the structure of B. bovis AMA-1 (BbAMA-1) has not yet been established. Herein, the present study aimed to express the recombinant BbAMA-1 domain I+II protein [rBbAMA-1(I/II)], which is the most predominant immune response region, and to characterize its immune response. As a result, cattle vaccinated with BbAMA-1(I/II) significantly developed high titters of total immunoglobulin (Ig) G antibodies and a high ratio of IgG2/IgG1 when compared to control groups. Interestingly, the BbAMA-1(I/II)-based formulations produced in our study could elicit CD4+ T cells and CD8+ T cells producing IFN-γ and tumor necrosis factor (TNF)-α. Collectively, the results indicate that immunization of cattle with BbAMA-1(I/II) could induce strong Th1 cell responses. In support of this, we observed the up-regulation of Th1 cytokine mRNA transcripts, including IFN-γ, TNF-α, Interleukin (IL)-2 and IL-12, in contrast to down regulation of IL-4, IL-6 and IL-10, which would be indicative of a Th2 cytokine response. Moreover, the up-regulation of inducible nitric oxide synthase (iNOS) was observed. In conclusion, this is the first report on the in-depth immunological characterization of the response to BbAMA-1. According to our results, BbAMA-1 is recognized as a potential candidate vaccine against B. bovis infection. As evidenced by the Th1 cell response, it could potentially provide protective immunity. However, further challenge-exposure with virulent B. bovis strain in immunized cattle would be needed to determine its protective efficacy. |
| first_indexed | 2024-12-12T17:23:56Z |
| format | Article |
| id | doaj.art-a9eb644c973440d4ae259ac213e16199 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-12T17:23:56Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-a9eb644c973440d4ae259ac213e161992022-12-22T00:17:35ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-06-01910.3389/fvets.2022.917389917389Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune ResponseAmarin Rittipornlertrak0Amarin Rittipornlertrak1Boondarika Nambooppha2Boondarika Nambooppha3Anucha Muenthaisong4Nisachon Apinda5Pongpisid Koonyosying6Wanwisa Srisawat7Paweena Chomjit8Kanokwan Sangkakam9Veerasak Punyapornwithaya10Saruda Tiwananthagorn11Naoaki Yokoyama12Nattawooti Sthitmatee13Nattawooti Sthitmatee14Nattawooti Sthitmatee15Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandRuminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandRuminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandExcellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, ThailandBoth strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4+ T cells and macrophages have been identified as major effector cells mediating the clearance of pathogens. Previously, the apical membrane antigen 1 (AMA-1) was found to significantly induce the immune response inhibiting B. bovis merozoite growth and invasion. However, a detailed characterization of both humoral and cellular immune responses against the structure of B. bovis AMA-1 (BbAMA-1) has not yet been established. Herein, the present study aimed to express the recombinant BbAMA-1 domain I+II protein [rBbAMA-1(I/II)], which is the most predominant immune response region, and to characterize its immune response. As a result, cattle vaccinated with BbAMA-1(I/II) significantly developed high titters of total immunoglobulin (Ig) G antibodies and a high ratio of IgG2/IgG1 when compared to control groups. Interestingly, the BbAMA-1(I/II)-based formulations produced in our study could elicit CD4+ T cells and CD8+ T cells producing IFN-γ and tumor necrosis factor (TNF)-α. Collectively, the results indicate that immunization of cattle with BbAMA-1(I/II) could induce strong Th1 cell responses. In support of this, we observed the up-regulation of Th1 cytokine mRNA transcripts, including IFN-γ, TNF-α, Interleukin (IL)-2 and IL-12, in contrast to down regulation of IL-4, IL-6 and IL-10, which would be indicative of a Th2 cytokine response. Moreover, the up-regulation of inducible nitric oxide synthase (iNOS) was observed. In conclusion, this is the first report on the in-depth immunological characterization of the response to BbAMA-1. According to our results, BbAMA-1 is recognized as a potential candidate vaccine against B. bovis infection. As evidenced by the Th1 cell response, it could potentially provide protective immunity. However, further challenge-exposure with virulent B. bovis strain in immunized cattle would be needed to determine its protective efficacy.https://www.frontiersin.org/articles/10.3389/fvets.2022.917389/fullBabesia bovisAMA-1recombinant proteinTh1 immune responsecattlevaccine |
| spellingShingle | Amarin Rittipornlertrak Amarin Rittipornlertrak Boondarika Nambooppha Boondarika Nambooppha Anucha Muenthaisong Nisachon Apinda Pongpisid Koonyosying Wanwisa Srisawat Paweena Chomjit Kanokwan Sangkakam Veerasak Punyapornwithaya Saruda Tiwananthagorn Naoaki Yokoyama Nattawooti Sthitmatee Nattawooti Sthitmatee Nattawooti Sthitmatee Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response Frontiers in Veterinary Science Babesia bovis AMA-1 recombinant protein Th1 immune response cattle vaccine |
| title | Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response |
| title_full | Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response |
| title_fullStr | Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response |
| title_full_unstemmed | Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response |
| title_short | Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response |
| title_sort | immunization of cattle with recombinant structural ectodomains i and ii of babesia bovis apical membrane antigen 1 bbama 1 i ii induces strong th1 immune response |
| topic | Babesia bovis AMA-1 recombinant protein Th1 immune response cattle vaccine |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.917389/full |
| work_keys_str_mv | AT amarinrittipornlertrak immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT amarinrittipornlertrak immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT boondarikanambooppha immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT boondarikanambooppha immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT anuchamuenthaisong immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT nisachonapinda immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT pongpisidkoonyosying immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT wanwisasrisawat immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT paweenachomjit immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT kanokwansangkakam immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT veerasakpunyapornwithaya immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT sarudatiwananthagorn immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT naoakiyokoyama immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT nattawootisthitmatee immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT nattawootisthitmatee immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse AT nattawootisthitmatee immunizationofcattlewithrecombinantstructuralectodomainsiandiiofbabesiabovisapicalmembraneantigen1bbama1iiiinducesstrongth1immuneresponse |