The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation
Abstract MORPHEUS’ MOLECULE1 (MOM1) is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we find that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with an artificial zinc finger to an unm...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-39751-4 |
| _version_ | 1797778781958569984 |
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| author | Zheng Li Ming Wang Zhenhui Zhong Javier Gallego-Bartolomé Suhua Feng Yasaman Jami-Alahmadi Xinyi Wang James Wohlschlegel Sylvain Bischof Jeff A. Long Steven E. Jacobsen |
| author_facet | Zheng Li Ming Wang Zhenhui Zhong Javier Gallego-Bartolomé Suhua Feng Yasaman Jami-Alahmadi Xinyi Wang James Wohlschlegel Sylvain Bischof Jeff A. Long Steven E. Jacobsen |
| author_sort | Zheng Li |
| collection | DOAJ |
| description | Abstract MORPHEUS’ MOLECULE1 (MOM1) is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we find that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with an artificial zinc finger to an unmethylated FWA promoter leads to establishment of DNA methylation and FWA silencing. This process is blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MICRORCHIDIA 6 (MORC6). We find that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes is impaired in the mom1 mutant. In addition to RdDM sites, we identify a group of MOM1 peaks at active chromatin near genes that colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation. |
| first_indexed | 2024-03-12T23:22:30Z |
| format | Article |
| id | doaj.art-a9ef9968972e4c4d834f28a8b57d10d9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-12T23:22:30Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-a9ef9968972e4c4d834f28a8b57d10d92023-07-16T11:21:39ZengNature PortfolioNature Communications2041-17232023-07-0114111610.1038/s41467-023-39751-4The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylationZheng Li0Ming Wang1Zhenhui Zhong2Javier Gallego-Bartolomé3Suhua Feng4Yasaman Jami-Alahmadi5Xinyi Wang6James Wohlschlegel7Sylvain Bischof8Jeff A. Long9Steven E. Jacobsen10Department of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Biological Chemistry, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Biological Chemistry, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell and Developmental Biology, University of CaliforniaAbstract MORPHEUS’ MOLECULE1 (MOM1) is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we find that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with an artificial zinc finger to an unmethylated FWA promoter leads to establishment of DNA methylation and FWA silencing. This process is blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MICRORCHIDIA 6 (MORC6). We find that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes is impaired in the mom1 mutant. In addition to RdDM sites, we identify a group of MOM1 peaks at active chromatin near genes that colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation.https://doi.org/10.1038/s41467-023-39751-4 |
| spellingShingle | Zheng Li Ming Wang Zhenhui Zhong Javier Gallego-Bartolomé Suhua Feng Yasaman Jami-Alahmadi Xinyi Wang James Wohlschlegel Sylvain Bischof Jeff A. Long Steven E. Jacobsen The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation Nature Communications |
| title | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
| title_full | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
| title_fullStr | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
| title_full_unstemmed | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
| title_short | The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation |
| title_sort | mom1 complex recruits the rddm machinery via morc6 to establish de novo dna methylation |
| url | https://doi.org/10.1038/s41467-023-39751-4 |
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