OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice
Abstract Leaf senescence, the last stage of leaf development, is essential for crop yield by promoting nutrition relocation from senescence leaves to new leaves and seeds. NAC (NAM/ATAF1/ATAF2/CUC2) proteins, one of the plant-specific transcription factors, widely distribute in plants and play impor...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-02-01
|
| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-024-00690-3 |
| _version_ | 1797273265659445248 |
|---|---|
| author | Lina Sun Hanqin Xu Juan Song Xiaoying Yang XinYi Wang Haiyan Liu Mengzhen Pang Youchuan Hu Qi Yang Xiaotong Ning Shanshan Liang Siju Zhang Weijiang Luan |
| author_facet | Lina Sun Hanqin Xu Juan Song Xiaoying Yang XinYi Wang Haiyan Liu Mengzhen Pang Youchuan Hu Qi Yang Xiaotong Ning Shanshan Liang Siju Zhang Weijiang Luan |
| author_sort | Lina Sun |
| collection | DOAJ |
| description | Abstract Leaf senescence, the last stage of leaf development, is essential for crop yield by promoting nutrition relocation from senescence leaves to new leaves and seeds. NAC (NAM/ATAF1/ATAF2/CUC2) proteins, one of the plant-specific transcription factors, widely distribute in plants and play important roles in plant growth and development. Here, we identified a new NAC member OsNAC103 and found that it plays critical roles in leaf senescence and plant architecture in rice. OsNAC103 mRNA levels were dramatically induced by leaf senescence as well as different phytohormones such as ABA, MeJA and ACC and abiotic stresses including dark, drought and high salinity. OsNAC103 acts as a transcription factor with nuclear localization signals at the N terminal and a transcriptional activation signal at the C terminal. Overexpression of OsNAC103 promoted leaf senescence while osnac103 mutants delayed leaf senescence under natural condition and dark-induced condition, meanwhile, senescence-associated genes (SAGs) were up-regulated in OsNAC103 overexpression (OsNAC103-OE) lines, indicating that OsNAC103 positively regulates leaf senescence in rice. Moreover, OsNAC103-OE lines exhibited loose plant architecture with larger tiller angles while tiller angles of osnac103 mutants decreased during the vegetative and reproductive growth stages due to the response of shoot gravitropism, suggesting that OsNAC103 can regulate the plant architecture in rice. Taken together, our results reveal that OsNAC103 plays crucial roles in the regulation of leaf senescence and plant architecture in rice. |
| first_indexed | 2024-03-07T14:41:03Z |
| format | Article |
| id | doaj.art-a9f0ca225bcf4f5c9f61a4f4d9cc3c0f |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-03-07T14:41:03Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-a9f0ca225bcf4f5c9f61a4f4d9cc3c0f2024-03-05T20:22:09ZengSpringerOpenRice1939-84251939-84332024-02-0117112110.1186/s12284-024-00690-3OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in RiceLina Sun0Hanqin Xu1Juan Song2Xiaoying Yang3XinYi Wang4Haiyan Liu5Mengzhen Pang6Youchuan Hu7Qi Yang8Xiaotong Ning9Shanshan Liang10Siju Zhang11Weijiang Luan12College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityCollege of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal UniversityAbstract Leaf senescence, the last stage of leaf development, is essential for crop yield by promoting nutrition relocation from senescence leaves to new leaves and seeds. NAC (NAM/ATAF1/ATAF2/CUC2) proteins, one of the plant-specific transcription factors, widely distribute in plants and play important roles in plant growth and development. Here, we identified a new NAC member OsNAC103 and found that it plays critical roles in leaf senescence and plant architecture in rice. OsNAC103 mRNA levels were dramatically induced by leaf senescence as well as different phytohormones such as ABA, MeJA and ACC and abiotic stresses including dark, drought and high salinity. OsNAC103 acts as a transcription factor with nuclear localization signals at the N terminal and a transcriptional activation signal at the C terminal. Overexpression of OsNAC103 promoted leaf senescence while osnac103 mutants delayed leaf senescence under natural condition and dark-induced condition, meanwhile, senescence-associated genes (SAGs) were up-regulated in OsNAC103 overexpression (OsNAC103-OE) lines, indicating that OsNAC103 positively regulates leaf senescence in rice. Moreover, OsNAC103-OE lines exhibited loose plant architecture with larger tiller angles while tiller angles of osnac103 mutants decreased during the vegetative and reproductive growth stages due to the response of shoot gravitropism, suggesting that OsNAC103 can regulate the plant architecture in rice. Taken together, our results reveal that OsNAC103 plays crucial roles in the regulation of leaf senescence and plant architecture in rice.https://doi.org/10.1186/s12284-024-00690-3NAC transcription factorLeaf senescencePlant architectureRice |
| spellingShingle | Lina Sun Hanqin Xu Juan Song Xiaoying Yang XinYi Wang Haiyan Liu Mengzhen Pang Youchuan Hu Qi Yang Xiaotong Ning Shanshan Liang Siju Zhang Weijiang Luan OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice Rice NAC transcription factor Leaf senescence Plant architecture Rice |
| title | OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice |
| title_full | OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice |
| title_fullStr | OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice |
| title_full_unstemmed | OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice |
| title_short | OsNAC103, a NAC Transcription Factor, Positively Regulates Leaf Senescence and Plant Architecture in Rice |
| title_sort | osnac103 a nac transcription factor positively regulates leaf senescence and plant architecture in rice |
| topic | NAC transcription factor Leaf senescence Plant architecture Rice |
| url | https://doi.org/10.1186/s12284-024-00690-3 |
| work_keys_str_mv | AT linasun osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT hanqinxu osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT juansong osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT xiaoyingyang osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT xinyiwang osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT haiyanliu osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT mengzhenpang osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT youchuanhu osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT qiyang osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT xiaotongning osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT shanshanliang osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT sijuzhang osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice AT weijiangluan osnac103anactranscriptionfactorpositivelyregulatesleafsenescenceandplantarchitectureinrice |