Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit
Globally, salinity and drought are severe abiotic stresses that presently threaten vegetable production. This study investigates the potential exogenously-applied glutathione (GSH) to relieve water deficits on Phaseolus vulgaris plants cultivated in saline soil conditions (6.22 dS m−1) by evaluating...
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PeerJ Inc.
2023-06-01
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| Online Access: | https://peerj.com/articles/15343.pdf |
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| author | Taia A. Abd El Mageed Wael Semida Khoulood A. Hemida Mohammed A.H. Gyushi Mostafa M. Rady Abdelsattar Abdelkhalik Othmane Merah Marian Brestic Heba I. Mohamed Ayman El Sabagh Magdi T. Abdelhamid |
| author_facet | Taia A. Abd El Mageed Wael Semida Khoulood A. Hemida Mohammed A.H. Gyushi Mostafa M. Rady Abdelsattar Abdelkhalik Othmane Merah Marian Brestic Heba I. Mohamed Ayman El Sabagh Magdi T. Abdelhamid |
| author_sort | Taia A. Abd El Mageed |
| collection | DOAJ |
| description | Globally, salinity and drought are severe abiotic stresses that presently threaten vegetable production. This study investigates the potential exogenously-applied glutathione (GSH) to relieve water deficits on Phaseolus vulgaris plants cultivated in saline soil conditions (6.22 dS m−1) by evaluating agronomic, stability index of membrane, water satatus, osmolytes, and antioxidant capacity responses. During two open field growing seasons (2017 and 2018), foliar spraying of glutathione (GSH) at 0.5 (GSH1) or 1.0 (GSH1) mM and three irrigation rates (I100 = 100%, I80 = 80% and I60 = 60% of the crop evapotranspiration) were applied to common bean plants. Water deficits significantly decreased common bean growth, green pods yield, integrity of the membranes, plant water status, SPAD chlorophyll index, and photosynthetic capacity (Fv/Fm, PI), while not improving the irrigation use efficiency (IUE) compared to full irrigation. Foliar-applied GSH markedly lessened drought-induced damages to bean plants, by enhancing the above variables. The integrative I80 + GSH1 or GSH2 and I60 + GSH1 or GSH2 elevated the IUE and exceeded the full irrigation without GSH application (I100) treatment by 38% and 37%, and 33% and 28%, respectively. Drought stress increased proline and total soluble sugars content while decreased the total free amino acids content. However, GSH-supplemented drought-stressed plants mediated further increases in all analyzed osmolytes contents. Exogenous GSH enhanced the common bean antioxidative machinery, being promoted the glutathione and ascorbic acid content as well as up-regulated the activity of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione peroxidase. These findings demonstrate the efficacy of exogenous GSH in alleviating water deficit in bean plants cultivated in salty soil. |
| first_indexed | 2024-03-09T04:09:54Z |
| format | Article |
| id | doaj.art-a9f6c3d0e86c4f018c31dee77f259630 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T04:09:54Z |
| publishDate | 2023-06-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-a9f6c3d0e86c4f018c31dee77f2596302023-12-03T14:01:22ZengPeerJ Inc.PeerJ2167-83592023-06-0111e1534310.7717/peerj.15343Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficitTaia A. Abd El Mageed0Wael Semida1Khoulood A. Hemida2Mohammed A.H. Gyushi3Mostafa M. Rady4Abdelsattar Abdelkhalik5Othmane Merah6Marian Brestic7Heba I. Mohamed8Ayman El Sabagh9Magdi T. Abdelhamid10Soil and Water Department, Faculty of Agriculture, Fayoum University, Fayoum, EgyptHorticulture Department, Faculty of Agriculture, Fayoum University, Fayoum, EgyptBotany Department, Faculty of Science, Fayoum University, Fayoum, EgyptHorticulture Department, Faculty of Agriculture, Fayoum University, Fayoum, EgyptBotany Department, Faculty of Agriculture, Fayoum University, Fayoum, EgyptHorticulture Department, Faculty of Agriculture, Fayoum University, Fayoum, EgyptLaboratoire de Chimie Agro-industrielle, Université de Toulouse, Toulouse, Toulouse, FrancePlant Physiology, Slovak University of Agriculture, Nitra, Nitra, SlovakiaBiological and Geological Sciences Department, Faculty of Education, Ain Shams University, Cairo, EgyptDepartment of Agronomy, Faculty of Agriculture, Kafrelsheikh University, Kafr Al-Sheik, EgyptBotany Department, National Research Centre, Cairo, EgyptGlobally, salinity and drought are severe abiotic stresses that presently threaten vegetable production. This study investigates the potential exogenously-applied glutathione (GSH) to relieve water deficits on Phaseolus vulgaris plants cultivated in saline soil conditions (6.22 dS m−1) by evaluating agronomic, stability index of membrane, water satatus, osmolytes, and antioxidant capacity responses. During two open field growing seasons (2017 and 2018), foliar spraying of glutathione (GSH) at 0.5 (GSH1) or 1.0 (GSH1) mM and three irrigation rates (I100 = 100%, I80 = 80% and I60 = 60% of the crop evapotranspiration) were applied to common bean plants. Water deficits significantly decreased common bean growth, green pods yield, integrity of the membranes, plant water status, SPAD chlorophyll index, and photosynthetic capacity (Fv/Fm, PI), while not improving the irrigation use efficiency (IUE) compared to full irrigation. Foliar-applied GSH markedly lessened drought-induced damages to bean plants, by enhancing the above variables. The integrative I80 + GSH1 or GSH2 and I60 + GSH1 or GSH2 elevated the IUE and exceeded the full irrigation without GSH application (I100) treatment by 38% and 37%, and 33% and 28%, respectively. Drought stress increased proline and total soluble sugars content while decreased the total free amino acids content. However, GSH-supplemented drought-stressed plants mediated further increases in all analyzed osmolytes contents. Exogenous GSH enhanced the common bean antioxidative machinery, being promoted the glutathione and ascorbic acid content as well as up-regulated the activity of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione peroxidase. These findings demonstrate the efficacy of exogenous GSH in alleviating water deficit in bean plants cultivated in salty soil.https://peerj.com/articles/15343.pdfAntioxidantDrought stressGrowthOsmotic stressWater statusYield |
| spellingShingle | Taia A. Abd El Mageed Wael Semida Khoulood A. Hemida Mohammed A.H. Gyushi Mostafa M. Rady Abdelsattar Abdelkhalik Othmane Merah Marian Brestic Heba I. Mohamed Ayman El Sabagh Magdi T. Abdelhamid Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit PeerJ Antioxidant Drought stress Growth Osmotic stress Water status Yield |
| title | Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit |
| title_full | Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit |
| title_fullStr | Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit |
| title_full_unstemmed | Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit |
| title_short | Glutathione-mediated changes in productivity, photosynthetic efficiency, osmolytes, and antioxidant capacity of common beans (Phaseolus vulgaris) grown under water deficit |
| title_sort | glutathione mediated changes in productivity photosynthetic efficiency osmolytes and antioxidant capacity of common beans phaseolus vulgaris grown under water deficit |
| topic | Antioxidant Drought stress Growth Osmotic stress Water status Yield |
| url | https://peerj.com/articles/15343.pdf |
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