Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis
Background: Adenoviral keratoconjunctivitis is a prevalent form of eye infection which involves the conjunctiva and less commonly the cornea. The signs and symptoms of the disease are more often overlapped with other causes of conjunctivitis. In this study, we applied polymerase chain reaction (PCR)...
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Isfahan University of Medical Sciences
2013-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2472 |
| _version_ | 1797728354457092096 |
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| author | Masoud Mokhtari Amir Reza Laripour Tehranfar Sharareh Moghim Ali Reza Dehghani Ziba Farajzadegan Nafiseh Sadat Hosseini Seyyed Ali Razavinasab |
| author_facet | Masoud Mokhtari Amir Reza Laripour Tehranfar Sharareh Moghim Ali Reza Dehghani Ziba Farajzadegan Nafiseh Sadat Hosseini Seyyed Ali Razavinasab |
| author_sort | Masoud Mokhtari |
| collection | DOAJ |
| description | Background: Adenoviral keratoconjunctivitis is a prevalent form of eye infection which involves the conjunctiva and less commonly the cornea. The signs and symptoms of the disease are more often overlapped with other causes of conjunctivitis. In this study, we applied polymerase chain reaction (PCR) as a sensitive test for diagnosis of adenovirus to determine the prevalence, sensitivity, and specificity of each sign and symptom of adenoviral keratoconjunctivitis.
Methods: This descriptive, analytic, cross-sectional study was conducted from September 2011 to September 2012 at Feiz Hospital of Ophthalmology and laboratory of virology, affiliated to Isfahan University of Medical Sciences, Iran. After obtaining ocular specimens from 195 patients with general signs and symptoms of conjunctivitis, their signs and symptoms were recorded. The specimens were tested by PCR to evaluate adenoviral etiology. Prevalence, sensitivity, and specificity of each sign or symptom was measured based on the findings of PCR.
Findings: The ocular specimens were collected from 106 (55%) males and 87(45%) females with the mean age of 27.3 ± 7.1 years old. The prevalence of adenoviral keratoconjunctivitis was 11.4 %. There was no significant difference in signs and symptoms between patients with positive and negative adenoviral PCR test. The most sensitive (100%) sign and symptom were conjunctival hyperemia and sudden onset, respectively. The least sensitive (0%) sign and symptom were blepharospasm and membrane/pseudomembrane formation, respectively. The most specific (98.84%) and the least specific (1.16%) were blepharospasm and conjunctival hyperemia, respectively.
Conclusion: Although many signs and symptoms accounted for adenoviral conjunctivitis, there was no hallmark for disease diagnosis. Conjunctival hyperemia is a sensitive criterion to diagnose adenoviral keratoconjunctivitis and there was no prevalent specific criterion for this purpose.
Keywords: Keratoconjunctivitis, Adenovirus, Sensitivity and specificity, Polymerase chain reaction |
| first_indexed | 2024-03-12T11:12:51Z |
| format | Article |
| id | doaj.art-a9f7c6c7fdd346b2a7d24d05464a8680 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T11:12:51Z |
| publishDate | 2013-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-a9f7c6c7fdd346b2a7d24d05464a86802023-09-02T02:30:59ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-01-0130215202920381212Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral KeratoconjunctivitisMasoud Mokhtari0Amir Reza Laripour Tehranfar1Sharareh Moghim2Ali Reza Dehghani3Ziba Farajzadegan4Nafiseh Sadat Hosseini5Seyyed Ali Razavinasab6Student of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranStudent of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Virology and Bacteriology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Ophthalmology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Community Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Virology and Bacteriology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranGeneral Practitioner, Research Assistant, Kerman University of Medical Sciences, Kerman, IranBackground: Adenoviral keratoconjunctivitis is a prevalent form of eye infection which involves the conjunctiva and less commonly the cornea. The signs and symptoms of the disease are more often overlapped with other causes of conjunctivitis. In this study, we applied polymerase chain reaction (PCR) as a sensitive test for diagnosis of adenovirus to determine the prevalence, sensitivity, and specificity of each sign and symptom of adenoviral keratoconjunctivitis. Methods: This descriptive, analytic, cross-sectional study was conducted from September 2011 to September 2012 at Feiz Hospital of Ophthalmology and laboratory of virology, affiliated to Isfahan University of Medical Sciences, Iran. After obtaining ocular specimens from 195 patients with general signs and symptoms of conjunctivitis, their signs and symptoms were recorded. The specimens were tested by PCR to evaluate adenoviral etiology. Prevalence, sensitivity, and specificity of each sign or symptom was measured based on the findings of PCR. Findings: The ocular specimens were collected from 106 (55%) males and 87(45%) females with the mean age of 27.3 ± 7.1 years old. The prevalence of adenoviral keratoconjunctivitis was 11.4 %. There was no significant difference in signs and symptoms between patients with positive and negative adenoviral PCR test. The most sensitive (100%) sign and symptom were conjunctival hyperemia and sudden onset, respectively. The least sensitive (0%) sign and symptom were blepharospasm and membrane/pseudomembrane formation, respectively. The most specific (98.84%) and the least specific (1.16%) were blepharospasm and conjunctival hyperemia, respectively. Conclusion: Although many signs and symptoms accounted for adenoviral conjunctivitis, there was no hallmark for disease diagnosis. Conjunctival hyperemia is a sensitive criterion to diagnose adenoviral keratoconjunctivitis and there was no prevalent specific criterion for this purpose. Keywords: Keratoconjunctivitis, Adenovirus, Sensitivity and specificity, Polymerase chain reactionhttp://jims.mui.ac.ir/index.php/jims/article/view/2472 |
| spellingShingle | Masoud Mokhtari Amir Reza Laripour Tehranfar Sharareh Moghim Ali Reza Dehghani Ziba Farajzadegan Nafiseh Sadat Hosseini Seyyed Ali Razavinasab Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis مجله دانشکده پزشکی اصفهان |
| title | Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis |
| title_full | Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis |
| title_fullStr | Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis |
| title_full_unstemmed | Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis |
| title_short | Determination of Prevalence, Sensitivity and Specificity of Clinical Signs and Symptoms of Adenoviral Keratoconjunctivitis |
| title_sort | determination of prevalence sensitivity and specificity of clinical signs and symptoms of adenoviral keratoconjunctivitis |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2472 |
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