The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility
Background The prognostic value of peripheral natural killer (pNK) cells, as a screening test in women with recur- rent pregnancy loss (RPL) and unexplained infertility, is still a matter for discussion. The purpose of this study was to compare the percentage of circulating CD56+NK cells, CD69 an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2019-04-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45517_90600e7e73f83f9718aca940b93593ad.pdf |
| _version_ | 1819091142954713088 |
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| author | Azam Azargoon Yasaman Mirrasouli Mahdieh Shokrollahi Barough Mehdi Barati Parviz Kokhaei |
| author_facet | Azam Azargoon Yasaman Mirrasouli Mahdieh Shokrollahi Barough Mehdi Barati Parviz Kokhaei |
| author_sort | Azam Azargoon |
| collection | DOAJ |
| description | Background The prognostic value of peripheral natural killer (pNK) cells, as a screening test in women with recur- rent pregnancy loss (RPL) and unexplained infertility, is still a matter for discussion. The purpose of this study was to compare the percentage of circulating CD56+NK cells, CD69 and perforin markers between women with unexplained infertility and RPL with the healthy control group. Materials and Methods In this case-control study, the percentage of CD56+NK cells and activation markers (CD69 and perforin levels) in the peripheral blood were measured in 25 women with unexplained infertility, 24 women with idiopathic RPL and 26 women from the healthy control group, using specific monoclonal antibodies by flow cytometry. Results The percentage of CD56+NK cells was significantly higher in patients with infertility in comparison with the healthy control group (P=0.007). There were not significant differences either in the total number of CD56+cells between the RPL group and the control group (P=0.2) or between the RPL group and the infertile group (P=0.36). The percentage of CD69+lymphocytes in RPL group was significantly higher than in the infertility group (P=0.004). There was a statistically significant difference in Perforin levels between RLP and control (P=0.001) as well as RPL and infertile (P=0.002) groups. Conclusion An increased percentage of CD56+NK cells in patients with unexplained infertility, an elevated expression of CD69 on NK cells in patients with RPL and infertility and a high level of perforin on CD56+cells in the RPL group might be considered as immunological risk factors in these women. |
| first_indexed | 2024-12-21T22:35:02Z |
| format | Article |
| id | doaj.art-a9f87574c4a14abc995dc79c620d52a6 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-21T22:35:02Z |
| publishDate | 2019-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-a9f87574c4a14abc995dc79c620d52a62022-12-21T18:47:59ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782019-04-01131121710.22074/ijfs.2019.550345517The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained InfertilityAzam Azargoon0Yasaman Mirrasouli1Mahdieh Shokrollahi Barough2Mehdi Barati3Parviz Kokhaei4Abnormal Uterine Bleeding Research Center, Semnan University of Medical Sciences, Semnan, Iran;Department of Infertility, Amir-AL-Momenin Hospital, Semnan University of Medical Sciences, Semnan, IranDepartment of Infertility, Amir-AL-Momenin Hospital, Semnan University of Medical Sciences, Semnan, Iran;Student Research Committee, Semnan University of Medical Sciences, Semnan, IranStudent Research Committee, Semnan University of Medical Sciences, Semnan, Iran;4Cancer Immunotherapy and Regenerative Medicine Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, IranStudent Research Committee, Semnan University of Medical Sciences, Semnan, Iran;5Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran6Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran;7Immune and Gene therapy Lab, CCK, Karolinska University Hospital Solna, Stockholm, SwedenBackground The prognostic value of peripheral natural killer (pNK) cells, as a screening test in women with recur- rent pregnancy loss (RPL) and unexplained infertility, is still a matter for discussion. The purpose of this study was to compare the percentage of circulating CD56+NK cells, CD69 and perforin markers between women with unexplained infertility and RPL with the healthy control group. Materials and Methods In this case-control study, the percentage of CD56+NK cells and activation markers (CD69 and perforin levels) in the peripheral blood were measured in 25 women with unexplained infertility, 24 women with idiopathic RPL and 26 women from the healthy control group, using specific monoclonal antibodies by flow cytometry. Results The percentage of CD56+NK cells was significantly higher in patients with infertility in comparison with the healthy control group (P=0.007). There were not significant differences either in the total number of CD56+cells between the RPL group and the control group (P=0.2) or between the RPL group and the infertile group (P=0.36). The percentage of CD69+lymphocytes in RPL group was significantly higher than in the infertility group (P=0.004). There was a statistically significant difference in Perforin levels between RLP and control (P=0.001) as well as RPL and infertile (P=0.002) groups. Conclusion An increased percentage of CD56+NK cells in patients with unexplained infertility, an elevated expression of CD69 on NK cells in patients with RPL and infertility and a high level of perforin on CD56+cells in the RPL group might be considered as immunological risk factors in these women.http://www.ijfs.ir/article_45517_90600e7e73f83f9718aca940b93593ad.pdfcd56infertilityperforinperipheral natural killer cellrecurrent miscarriage |
| spellingShingle | Azam Azargoon Yasaman Mirrasouli Mahdieh Shokrollahi Barough Mehdi Barati Parviz Kokhaei The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility International Journal of Fertility and Sterility cd56 infertility perforin peripheral natural killer cell recurrent miscarriage |
| title | The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility |
| title_full | The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility |
| title_fullStr | The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility |
| title_full_unstemmed | The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility |
| title_short | The State of Peripheral Blood Natural Killer Cells and Cytotoxicity in Women with Recurrent Pregnancy Loss and Unexplained Infertility |
| title_sort | state of peripheral blood natural killer cells and cytotoxicity in women with recurrent pregnancy loss and unexplained infertility |
| topic | cd56 infertility perforin peripheral natural killer cell recurrent miscarriage |
| url | http://www.ijfs.ir/article_45517_90600e7e73f83f9718aca940b93593ad.pdf |
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