Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma
Objective: Multiple myeloma (MM) is a malignant condition characterized by the accumulation of malignant plasma cells. Although MM remains incurable, the survival of MM patients has improved considerably due to the application of autologous stem cell transplantation, novel agents, and advanced treat...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2022-05-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-67026 |
| _version_ | 1797909123492216832 |
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| author | Ahmet Seyhanlı Boran Yavuz Zehra Akşit Zeynep Yüce Sermin Özkal Oğuz Altungöz Fatih Demirkan İnci Alacacıoğlu Güner Hayri Özsan |
| author_facet | Ahmet Seyhanlı Boran Yavuz Zehra Akşit Zeynep Yüce Sermin Özkal Oğuz Altungöz Fatih Demirkan İnci Alacacıoğlu Güner Hayri Özsan |
| author_sort | Ahmet Seyhanlı |
| collection | DOAJ |
| description | Objective: Multiple myeloma (MM) is a malignant condition characterized by the accumulation of malignant plasma cells. Although MM remains incurable, the survival of MM patients has improved considerably due to the application of autologous stem cell transplantation, novel agents, and advanced treatment strategies. This study aimed to determine the cytogenetic characterization and bone marrow (BM) features of Turkish patients with MM. Materials and Methods: Eighty-five MM patients were admitted to Dokuz Eylül University Hospital in Turkey. BM samples of these MM patients were subjected to cytogenetic analyses at diagnosis and during therapy as a part of therapeutical and clinical evaluation. A complete cytogenetic study was performed using the G-banding technique. Fluorescence in situ hybridization (FISH) analysis was performed using cytoplasmic immunoglobulin. The degree of BM fibrosis was determined using reticulin histochemical staining. We determined the percentage of BM plasma cells based on the extent of CD38 staining. Results: Eighty-five MM patients were retrospectively identified between 2015 and 2021. The median age was 63 (38-90) years. Of the 85 patients, 60 (70.6%) were male and 25 (29.4%) were female. Seventy-two (84.7%) cases had BM fibrosis at the time of diagnosis. The most common was grade 2 fibrosis, recorded in 35 cases (41.2%). About 72.9% of the patients showed more than 50% plasma cells. FISH analysis indicated the presence of abnormal chromosomes in 37% (32/85) of the patients. The most frequent abnormality was Immunoglobulin heavy-chain (IGH) translocation (21.3%). Conclusion: Subgroup analysis of IGH mutations is crucial in the identification of high-risk MM patients. We believe that our study will contribute to the determination of BM biopsy and cytogenetic features of MM patients in our country. |
| first_indexed | 2024-04-10T11:04:47Z |
| format | Article |
| id | doaj.art-a9f9a3e8d1444c2c9d9e8ff29832771c |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:04:47Z |
| publishDate | 2022-05-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-a9f9a3e8d1444c2c9d9e8ff29832771c2023-02-15T16:19:31ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632022-05-0139210911610.4274/tjh.galenos.2021.2021.0325TJH-67026Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple MyelomaAhmet Seyhanlı0Boran Yavuz1Zehra Akşit2Zeynep Yüce3Sermin Özkal4Oğuz Altungöz5Fatih Demirkan6İnci Alacacıoğlu7Güner Hayri Özsan8Sivas Numune Hospital, Clinic of Hematology, Sivas, TurkeyDokuz Eylül University Faculty of Medicine, Department of Hematology, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Internal Medicine, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Medical Biology, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Pathology, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Medical Biology, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Hematology, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Hematology, İzmir, TurkeyDokuz Eylül University Faculty of Medicine, Department of Hematology, İzmir, TurkeyObjective: Multiple myeloma (MM) is a malignant condition characterized by the accumulation of malignant plasma cells. Although MM remains incurable, the survival of MM patients has improved considerably due to the application of autologous stem cell transplantation, novel agents, and advanced treatment strategies. This study aimed to determine the cytogenetic characterization and bone marrow (BM) features of Turkish patients with MM. Materials and Methods: Eighty-five MM patients were admitted to Dokuz Eylül University Hospital in Turkey. BM samples of these MM patients were subjected to cytogenetic analyses at diagnosis and during therapy as a part of therapeutical and clinical evaluation. A complete cytogenetic study was performed using the G-banding technique. Fluorescence in situ hybridization (FISH) analysis was performed using cytoplasmic immunoglobulin. The degree of BM fibrosis was determined using reticulin histochemical staining. We determined the percentage of BM plasma cells based on the extent of CD38 staining. Results: Eighty-five MM patients were retrospectively identified between 2015 and 2021. The median age was 63 (38-90) years. Of the 85 patients, 60 (70.6%) were male and 25 (29.4%) were female. Seventy-two (84.7%) cases had BM fibrosis at the time of diagnosis. The most common was grade 2 fibrosis, recorded in 35 cases (41.2%). About 72.9% of the patients showed more than 50% plasma cells. FISH analysis indicated the presence of abnormal chromosomes in 37% (32/85) of the patients. The most frequent abnormality was Immunoglobulin heavy-chain (IGH) translocation (21.3%). Conclusion: Subgroup analysis of IGH mutations is crucial in the identification of high-risk MM patients. We believe that our study will contribute to the determination of BM biopsy and cytogenetic features of MM patients in our country.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-67026multiple myelomabone marrow biopsycytogenetic analysisfluorescence in situ hybridization |
| spellingShingle | Ahmet Seyhanlı Boran Yavuz Zehra Akşit Zeynep Yüce Sermin Özkal Oğuz Altungöz Fatih Demirkan İnci Alacacıoğlu Güner Hayri Özsan Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma Turkish Journal of Hematology multiple myeloma bone marrow biopsy cytogenetic analysis fluorescence in situ hybridization |
| title | Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma |
| title_full | Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma |
| title_fullStr | Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma |
| title_full_unstemmed | Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma |
| title_short | Assessment of Bone Marrow Biopsy and Cytogenetic Findings in Patients with Multiple Myeloma |
| title_sort | assessment of bone marrow biopsy and cytogenetic findings in patients with multiple myeloma |
| topic | multiple myeloma bone marrow biopsy cytogenetic analysis fluorescence in situ hybridization |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-67026 |
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