Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy
The present study explores the green approach for the preparation of silver nanoparticles (AgNPs) through the reduction of silver nitrate by the cell-free stem and leaf aqueous extracts of Litsea glutinosa (L.glutinosa) and its potential antibacterial activity. The analytical instruments include sca...
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| Format: | Article |
| Language: | English |
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Iranian Environmental Mutagen Society
2022-12-01
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| Series: | Journal of Water and Environmental Nanotechnology |
| Subjects: | |
| Online Access: | http://www.jwent.net/article_700843_02f9e380db34427d275971d41de681bc.pdf |
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| author | P. Koteswara Rao B Vikram Babu A. Rama Krishna M. Sushma Reddi B. Sathish Mohan K. Anjani Devi U. Susmitha T. Raghava Rao |
| author_facet | P. Koteswara Rao B Vikram Babu A. Rama Krishna M. Sushma Reddi B. Sathish Mohan K. Anjani Devi U. Susmitha T. Raghava Rao |
| author_sort | P. Koteswara Rao |
| collection | DOAJ |
| description | The present study explores the green approach for the preparation of silver nanoparticles (AgNPs) through the reduction of silver nitrate by the cell-free stem and leaf aqueous extracts of Litsea glutinosa (L.glutinosa) and its potential antibacterial activity. The analytical instruments include scanning electron microscopy, Fourier transforms infrared spectroscopy, UV-visible spectroscopy, and X-ray diffraction spectroscopy confirmed the synthesis of smaller, uniformly spherical AgNPs (10-40 nm). The average crystalline size of prepared AgNPs produced by L. glutinosa leaf extract was found to be 19 mm. From UV-visible spectral analysis, the maximum absorbance peak appeared at 444 nm for leaf extract AgNPs different from stem extract AgNPs (422 nm), which are found to be specific for AgNPs. The L.glutinosa stem extract-assisted AgNPs have shown significant antibacterial activity against Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) in comparison to Gentamycin. Hence, the AgNPs obtained by green synthesis can be therapeutically explored against bacterial infections. |
| first_indexed | 2024-04-09T15:14:11Z |
| format | Article |
| id | doaj.art-aa0080de4042421d888fb00740444370 |
| institution | Directory Open Access Journal |
| issn | 2476-7204 2476-6615 |
| language | English |
| last_indexed | 2024-04-09T15:14:11Z |
| publishDate | 2022-12-01 |
| publisher | Iranian Environmental Mutagen Society |
| record_format | Article |
| series | Journal of Water and Environmental Nanotechnology |
| spelling | doaj.art-aa0080de4042421d888fb007404443702023-04-30T07:04:30ZengIranian Environmental Mutagen SocietyJournal of Water and Environmental Nanotechnology2476-72042476-66152022-12-017436336910.22090/jwent.2022.04.003700843Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial EfficacyP. Koteswara Rao0B Vikram Babu1A. Rama Krishna2M. Sushma Reddi3B. Sathish Mohan4K. Anjani Devi5U. Susmitha6T. Raghava Rao7Department of Biochemistry, Andhra University, Visakhapatnam-530003, IndiaDepartment of Physics, International School of Technology and Sciences (ISTS) for Women, Rajanagaram, Rajamahendravaram, Andhra Pradesh 533294, India. Aditya Engineering College (A), Kakinada, 533005, IndiaDepartment of ECE, Aditya College of Engineering and Technology, Surampalem, IndiaDepartment of Physics, Dr. B. R. Ambedkar University, Srikakulam- 532410, IndiaBio Enviro Chemical Solutions, Visakhapatnam-530017, IndiaDepartment of Physics, Aditya College of Engineering and Technology, Surampalem, IndiaQuiver Biotech Pvt Ltd., Mallapur, Hyderabad-500070, IndiaDepartment of Biochemistry, Andhra University, Visakhapatnam-530003, IndiaThe present study explores the green approach for the preparation of silver nanoparticles (AgNPs) through the reduction of silver nitrate by the cell-free stem and leaf aqueous extracts of Litsea glutinosa (L.glutinosa) and its potential antibacterial activity. The analytical instruments include scanning electron microscopy, Fourier transforms infrared spectroscopy, UV-visible spectroscopy, and X-ray diffraction spectroscopy confirmed the synthesis of smaller, uniformly spherical AgNPs (10-40 nm). The average crystalline size of prepared AgNPs produced by L. glutinosa leaf extract was found to be 19 mm. From UV-visible spectral analysis, the maximum absorbance peak appeared at 444 nm for leaf extract AgNPs different from stem extract AgNPs (422 nm), which are found to be specific for AgNPs. The L.glutinosa stem extract-assisted AgNPs have shown significant antibacterial activity against Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) in comparison to Gentamycin. Hence, the AgNPs obtained by green synthesis can be therapeutically explored against bacterial infections.http://www.jwent.net/article_700843_02f9e380db34427d275971d41de681bc.pdfgreen synthesisl.glutinosaleaf extractagnpsantibacterial activity |
| spellingShingle | P. Koteswara Rao B Vikram Babu A. Rama Krishna M. Sushma Reddi B. Sathish Mohan K. Anjani Devi U. Susmitha T. Raghava Rao Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy Journal of Water and Environmental Nanotechnology green synthesis l.glutinosa leaf extract agnps antibacterial activity |
| title | Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy |
| title_full | Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy |
| title_fullStr | Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy |
| title_full_unstemmed | Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy |
| title_short | Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy |
| title_sort | green synthesis of silver nanoparticles using litsea glutinosa l leaves and stem extracts and their antibacterial efficacy |
| topic | green synthesis l.glutinosa leaf extract agnps antibacterial activity |
| url | http://www.jwent.net/article_700843_02f9e380db34427d275971d41de681bc.pdf |
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