Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation
The neural crest (NC) is a transient population of embryonic progenitors that are implicated in a diverse range of congenital birth defects and pediatric syndromes. The broad spectrum of NC-related disorders can be attributed to the wide variety of differentiated cell types arising from the NC. In v...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2018-12-01
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| Series: | Disease Models & Mechanisms |
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| Online Access: | http://dmm.biologists.org/content/11/12/dmm035097 |
| _version_ | 1819040791972020224 |
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| author | Maria R. Replogle Virinchipuram S. Sreevidya Vivian M. Lee Michael D. Laiosa Kurt R. Svoboda Ava J. Udvadia |
| author_facet | Maria R. Replogle Virinchipuram S. Sreevidya Vivian M. Lee Michael D. Laiosa Kurt R. Svoboda Ava J. Udvadia |
| author_sort | Maria R. Replogle |
| collection | DOAJ |
| description | The neural crest (NC) is a transient population of embryonic progenitors that are implicated in a diverse range of congenital birth defects and pediatric syndromes. The broad spectrum of NC-related disorders can be attributed to the wide variety of differentiated cell types arising from the NC. In vitro models of NC development provide a powerful platform for testing the relative contributions of intrinsic and extrinsic factors mediating NC differentiation under normal and pathogenic conditions. Although differentiation is a dynamic process that unfolds over time, currently, there is no well-defined chronology that characterizes the in vitro progression of NC differentiation towards specific cell fates. In this study, we have optimized culture conditions for expansion of primary murine NC cells that give rise to both ectodermal and mesoectodermal derivatives, even after multiple passages. Significantly, we have delineated highly reproducible timelines that include distinct intermediate stages for lineage-specific NC differentiation in vitro. In addition, isolating both cranial and trunk NC cells from the same embryos enabled us to make direct comparisons between the two cell populations over the course of differentiation. Our results define characteristic changes in cell morphology and behavior that track the temporal progression of NC cells as they differentiate along the neuronal, glial and chondrogenic lineages in vitro. These benchmarks constitute a chronological baseline for assessing how genetic or environmental disruptions may facilitate or impede NC differentiation. Introducing a temporal dimension substantially increases the power of this platform for screening drugs or chemicals for developmental toxicity or therapeutic potential.
This article has an associated First Person interview with the first author of the paper. |
| first_indexed | 2024-12-21T09:14:43Z |
| format | Article |
| id | doaj.art-aa010c50def04a32b6e589bd8499de4f |
| institution | Directory Open Access Journal |
| issn | 1754-8403 1754-8411 |
| language | English |
| last_indexed | 2024-12-21T09:14:43Z |
| publishDate | 2018-12-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Disease Models & Mechanisms |
| spelling | doaj.art-aa010c50def04a32b6e589bd8499de4f2022-12-21T19:09:11ZengThe Company of BiologistsDisease Models & Mechanisms1754-84031754-84112018-12-01111210.1242/dmm.035097035097Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiationMaria R. Replogle0Virinchipuram S. Sreevidya1Vivian M. Lee2Michael D. Laiosa3Kurt R. Svoboda4Ava J. Udvadia5 Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA Joseph J. Zilber School of Public Health, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA STEMCELL Technologies, Vancouver, BC V6A 1BC, Canada Joseph J. Zilber School of Public Health, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA Joseph J. Zilber School of Public Health, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA The neural crest (NC) is a transient population of embryonic progenitors that are implicated in a diverse range of congenital birth defects and pediatric syndromes. The broad spectrum of NC-related disorders can be attributed to the wide variety of differentiated cell types arising from the NC. In vitro models of NC development provide a powerful platform for testing the relative contributions of intrinsic and extrinsic factors mediating NC differentiation under normal and pathogenic conditions. Although differentiation is a dynamic process that unfolds over time, currently, there is no well-defined chronology that characterizes the in vitro progression of NC differentiation towards specific cell fates. In this study, we have optimized culture conditions for expansion of primary murine NC cells that give rise to both ectodermal and mesoectodermal derivatives, even after multiple passages. Significantly, we have delineated highly reproducible timelines that include distinct intermediate stages for lineage-specific NC differentiation in vitro. In addition, isolating both cranial and trunk NC cells from the same embryos enabled us to make direct comparisons between the two cell populations over the course of differentiation. Our results define characteristic changes in cell morphology and behavior that track the temporal progression of NC cells as they differentiate along the neuronal, glial and chondrogenic lineages in vitro. These benchmarks constitute a chronological baseline for assessing how genetic or environmental disruptions may facilitate or impede NC differentiation. Introducing a temporal dimension substantially increases the power of this platform for screening drugs or chemicals for developmental toxicity or therapeutic potential. This article has an associated First Person interview with the first author of the paper.http://dmm.biologists.org/content/11/12/dmm035097Chondrogenic differentiationDifferentiation timelineGlial differentiationNeuronal differentiationSox9In vitro model of neural crest differentiation |
| spellingShingle | Maria R. Replogle Virinchipuram S. Sreevidya Vivian M. Lee Michael D. Laiosa Kurt R. Svoboda Ava J. Udvadia Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation Disease Models & Mechanisms Chondrogenic differentiation Differentiation timeline Glial differentiation Neuronal differentiation Sox9 In vitro model of neural crest differentiation |
| title | Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation |
| title_full | Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation |
| title_fullStr | Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation |
| title_full_unstemmed | Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation |
| title_short | Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation |
| title_sort | establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation |
| topic | Chondrogenic differentiation Differentiation timeline Glial differentiation Neuronal differentiation Sox9 In vitro model of neural crest differentiation |
| url | http://dmm.biologists.org/content/11/12/dmm035097 |
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