In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials
Abstract This study investigated the characterization, antifungal activity, and biocompatibility of green agar/silver and collagen/silver bionanocomposite films for wound healing and cell growth scaffolds. Silver nanoparticles (AgNPs) are known for their antimicrobial properties, but their toxicity...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-02-01
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| Series: | Nano Select |
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| Online Access: | https://doi.org/10.1002/nano.202300087 |
| _version_ | 1797319701447049216 |
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| author | Federico Trotta Alna Dony Monica Mok Alessandra Grillo Thomas Whitehead‐Clarke Shervanthi Homer‐Vanniasinkam Alvena Kureshi |
| author_facet | Federico Trotta Alna Dony Monica Mok Alessandra Grillo Thomas Whitehead‐Clarke Shervanthi Homer‐Vanniasinkam Alvena Kureshi |
| author_sort | Federico Trotta |
| collection | DOAJ |
| description | Abstract This study investigated the characterization, antifungal activity, and biocompatibility of green agar/silver and collagen/silver bionanocomposite films for wound healing and cell growth scaffolds. Silver nanoparticles (AgNPs) are known for their antimicrobial properties, but their toxicity and harsh synthesis limit their applications. To address this, green‐synthesized AgNPs G‐AgNPs were incorporated into agar/collagen suspensions at specific concentrations and three different G‐AgNP‐agar and two different G‐AgNP‐col bionanocomposite films were produced. Nanoparticle homogeneity and film quality were characterized through SEM analysis. Mechanical properties were tested using a uniaxial tensile tester, revealing that the bioplastic control samples exhibited UTS of 3.86 MPa compared to 0.60 MPa for collagen, a 6‐fold improvement. Viable cell metabolic activity derived from MTT assay showed that Col‐4%AgNPs and Bio‐30%AgNPs had a 42.9% and 51.6% increase in net metabolic activity respectively compared to control on day 4. Fluorescence microscopy confirmed enhanced cell adhesion and proliferation in G‐AgNP‐incorporated samples. Antifungal properties were evaluated against Cladosporium spores, able to cause severe diseases when in contact with human skins, following ISO 16869:2008 standards. The demonstrated unique properties and tunability of G‐AgNPs bionanocomposites can be employed in a variety of specialties for wound‐healing applications, to improve rate and quality of healing while reducing the risk of infection. |
| first_indexed | 2024-03-08T04:10:46Z |
| format | Article |
| id | doaj.art-aa02674a851a40e3b20dcf2cc4369970 |
| institution | Directory Open Access Journal |
| issn | 2688-4011 |
| language | English |
| last_indexed | 2024-03-08T04:10:46Z |
| publishDate | 2024-02-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Nano Select |
| spelling | doaj.art-aa02674a851a40e3b20dcf2cc43699702024-02-08T20:30:42ZengWiley-VCHNano Select2688-40112024-02-0152n/an/a10.1002/nano.202300087In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materialsFederico Trotta0Alna Dony1Monica Mok2Alessandra Grillo3Thomas Whitehead‐Clarke4Shervanthi Homer‐Vanniasinkam5Alvena Kureshi6Metalchemy Limited London UKMetalchemy Limited London UKMetalchemy Limited London UKCentre for 3D Models of Health and Disease Division of Surgery and Interventional Science University College London London UKCentre for 3D Models of Health and Disease Division of Surgery and Interventional Science University College London London UKDepartment of Mechanical Engineering University College London London UKCentre for 3D Models of Health and Disease Division of Surgery and Interventional Science University College London London UKAbstract This study investigated the characterization, antifungal activity, and biocompatibility of green agar/silver and collagen/silver bionanocomposite films for wound healing and cell growth scaffolds. Silver nanoparticles (AgNPs) are known for their antimicrobial properties, but their toxicity and harsh synthesis limit their applications. To address this, green‐synthesized AgNPs G‐AgNPs were incorporated into agar/collagen suspensions at specific concentrations and three different G‐AgNP‐agar and two different G‐AgNP‐col bionanocomposite films were produced. Nanoparticle homogeneity and film quality were characterized through SEM analysis. Mechanical properties were tested using a uniaxial tensile tester, revealing that the bioplastic control samples exhibited UTS of 3.86 MPa compared to 0.60 MPa for collagen, a 6‐fold improvement. Viable cell metabolic activity derived from MTT assay showed that Col‐4%AgNPs and Bio‐30%AgNPs had a 42.9% and 51.6% increase in net metabolic activity respectively compared to control on day 4. Fluorescence microscopy confirmed enhanced cell adhesion and proliferation in G‐AgNP‐incorporated samples. Antifungal properties were evaluated against Cladosporium spores, able to cause severe diseases when in contact with human skins, following ISO 16869:2008 standards. The demonstrated unique properties and tunability of G‐AgNPs bionanocomposites can be employed in a variety of specialties for wound‐healing applications, to improve rate and quality of healing while reducing the risk of infection.https://doi.org/10.1002/nano.202300087bionanocompositecolloidal silvergreen‐chemistrysilver nanoparticlessustainable biomaterialstissue engineering |
| spellingShingle | Federico Trotta Alna Dony Monica Mok Alessandra Grillo Thomas Whitehead‐Clarke Shervanthi Homer‐Vanniasinkam Alvena Kureshi In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials Nano Select bionanocomposite colloidal silver green‐chemistry silver nanoparticles sustainable biomaterials tissue engineering |
| title | In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials |
| title_full | In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials |
| title_fullStr | In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials |
| title_full_unstemmed | In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials |
| title_short | In vitro characterization of bionanocomposites with green silver nanoparticles: A step towards sustainable wound healing materials |
| title_sort | in vitro characterization of bionanocomposites with green silver nanoparticles a step towards sustainable wound healing materials |
| topic | bionanocomposite colloidal silver green‐chemistry silver nanoparticles sustainable biomaterials tissue engineering |
| url | https://doi.org/10.1002/nano.202300087 |
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