Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’
The Chinese plum (<i>Prunus salicina</i> L.) is a fruit tree belonging to the Rosaceae family, native to south-eastern China and widely cultivated throughout the world. Fruit sugar metabolism and color change is an important physiological behavior that directly determines flavor and arom...
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MDPI AG
2023-10-01
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| author | Zhimin Lin Xiaoyan Yi Muhammad Moaaz Ali Lijuan Zhang Shaojuan Wang Faxing Chen |
| author_facet | Zhimin Lin Xiaoyan Yi Muhammad Moaaz Ali Lijuan Zhang Shaojuan Wang Faxing Chen |
| author_sort | Zhimin Lin |
| collection | DOAJ |
| description | The Chinese plum (<i>Prunus salicina</i> L.) is a fruit tree belonging to the Rosaceae family, native to south-eastern China and widely cultivated throughout the world. Fruit sugar metabolism and color change is an important physiological behavior that directly determines flavor and aroma. Our study analyzed six stages of fruit growth and development using RNA-seq, yielding a total of 14,973 DEGs, and further evaluation of key DEGs revealed a focus on sugar metabolism, flavonoid biosynthesis, carotenoid biosynthesis, and photosynthesis. Using GO and KEGG to enrich differential genes in the pathway, we selected 107 differential genes and obtained 49 significant differential genes related to glucose metabolism. The results of the correlation analyses indicated that two genes of the SWEET family, evm.TU.Chr1.3663 (<i>PsSWEET9</i>) and evm.TU.Chr4.676 (<i>PsSWEET2</i>), could be closely related to the composition of soluble sugars, which was also confirmed in the ethylene treatment experiments. In addition, analysis of the TOP 20 pathways between different growth stages and the green stage, as well as transient overexpression in chili, suggested that capsanthin/capsorubin synthase (PsCCS) of the carotenoid biosynthetic pathway contributed to the color change of plum fruit. These findings provide an insight into the molecular mechanisms involved in the ripening and color change of plum fruit. |
| first_indexed | 2024-03-10T21:37:06Z |
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| last_indexed | 2024-03-10T21:37:06Z |
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| spelling | doaj.art-aa0777878e954df0a9bbf307aa1dffc32023-11-19T14:55:26ZengMDPI AGPlants2223-77472023-10-011219351310.3390/plants12193513Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’Zhimin Lin0Xiaoyan Yi1Muhammad Moaaz Ali2Lijuan Zhang3Shaojuan Wang4Faxing Chen5Fujian Academy of Agricultural Sciences Biotechnology Institute, Fuzhou 350003, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe Chinese plum (<i>Prunus salicina</i> L.) is a fruit tree belonging to the Rosaceae family, native to south-eastern China and widely cultivated throughout the world. Fruit sugar metabolism and color change is an important physiological behavior that directly determines flavor and aroma. Our study analyzed six stages of fruit growth and development using RNA-seq, yielding a total of 14,973 DEGs, and further evaluation of key DEGs revealed a focus on sugar metabolism, flavonoid biosynthesis, carotenoid biosynthesis, and photosynthesis. Using GO and KEGG to enrich differential genes in the pathway, we selected 107 differential genes and obtained 49 significant differential genes related to glucose metabolism. The results of the correlation analyses indicated that two genes of the SWEET family, evm.TU.Chr1.3663 (<i>PsSWEET9</i>) and evm.TU.Chr4.676 (<i>PsSWEET2</i>), could be closely related to the composition of soluble sugars, which was also confirmed in the ethylene treatment experiments. In addition, analysis of the TOP 20 pathways between different growth stages and the green stage, as well as transient overexpression in chili, suggested that capsanthin/capsorubin synthase (PsCCS) of the carotenoid biosynthetic pathway contributed to the color change of plum fruit. These findings provide an insight into the molecular mechanisms involved in the ripening and color change of plum fruit.https://www.mdpi.com/2223-7747/12/19/3513<i>Prunus salicina</i>sugar metabolismcarotenoid biosynthesisfruit developmenttranscriptome |
| spellingShingle | Zhimin Lin Xiaoyan Yi Muhammad Moaaz Ali Lijuan Zhang Shaojuan Wang Faxing Chen Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’ Plants <i>Prunus salicina</i> sugar metabolism carotenoid biosynthesis fruit development transcriptome |
| title | Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’ |
| title_full | Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’ |
| title_fullStr | Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’ |
| title_full_unstemmed | Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’ |
| title_short | Transcriptome Insights into Candidate Genes of the <i>SWEET</i> Family and Carotenoid Biosynthesis during Fruit Growth and Development in <i>Prunus salicina</i> ‘Huangguan’ |
| title_sort | transcriptome insights into candidate genes of the i sweet i family and carotenoid biosynthesis during fruit growth and development in i prunus salicina i huangguan |
| topic | <i>Prunus salicina</i> sugar metabolism carotenoid biosynthesis fruit development transcriptome |
| url | https://www.mdpi.com/2223-7747/12/19/3513 |
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