Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives

Abstract Background It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS). Aim This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application of self‐etching primers/adhesives (SEP...

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Main Authors: Stefanie Amend, Roland Frankenberger, Christina Boutsiouki, Vanessa Scharrelmann, Julia Winter, Norbert Krämer
Format: Article
Language:English
Published: Wiley 2021-10-01
Series:Clinical and Experimental Dental Research
Subjects:
Online Access:https://doi.org/10.1002/cre2.420
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author Stefanie Amend
Roland Frankenberger
Christina Boutsiouki
Vanessa Scharrelmann
Julia Winter
Norbert Krämer
author_facet Stefanie Amend
Roland Frankenberger
Christina Boutsiouki
Vanessa Scharrelmann
Julia Winter
Norbert Krämer
author_sort Stefanie Amend
collection DOAJ
description Abstract Background It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS). Aim This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application of self‐etching primers/adhesives (SEPA). Design 131 third molars were assigned to ten groups. In Hel‐P, Helioseal® was applied after phosphoric acid etching. In the other groups, SEPA were used (Dyr‐AP: Adper™ Prompt™ L‐Pop™, Dyract® Seal; Bea: BeautiSealant Primer and Paste; Hel‐Exp: Experimental primer, Helioseal®; Hel‐Cl: Clearfil™ SE Bond1, Helioseal®). Specimens were stored in distilled water at 37°C (28 days), followed by 3500 thermocycles and staining with 5% methylene blue (M) or 5% silver nitrate (S). After methylene blue staining and sectioning, microleakage was assessed light microscopically. During silver nitrate staining, specimens were dissolved by 32% HCl and remaining PFS were evaluated under a SEM. Results Tightness, percentage of penetrated area, and maximum dye penetration were best for Hel‐P and Hel‐Cl (p < 0.05). Conclusions Phosphoric acid etching of enamel and Clearfil™ SE Bond resulted in the best sealing quality. Methylene blue staining allowed the evaluation of more criteria (fissure shape, voids, sealant penetration depth) compared to silver nitrate.
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spelling doaj.art-aa094090c63b4a2b8ecb06b58d3fcf9d2022-12-21T19:14:21ZengWileyClinical and Experimental Dental Research2057-43472021-10-017576377110.1002/cre2.420Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesivesStefanie Amend0Roland Frankenberger1Christina Boutsiouki2Vanessa Scharrelmann3Julia Winter4Norbert Krämer5Department of Pediatric Dentistry, Dental School University of Marburg and University Medical Center Giessen and Marburg, Campus Giessen Giessen GermanyDepartment of Operative Dentistry, Endodontics, and Pediatric Dentistry, Dental School University of Marburg and University Medical Center Giessen and Marburg, Campus Marburg Marburg GermanyDepartment of Pediatric Dentistry, Dental School University of Marburg and University Medical Center Giessen and Marburg, Campus Giessen Giessen GermanyDepartment of Pediatric Dentistry, Dental School University of Marburg and University Medical Center Giessen and Marburg, Campus Giessen Giessen GermanyDepartment of Operative Dentistry, Endodontics, and Pediatric Dentistry, Dental School University of Marburg and University Medical Center Giessen and Marburg, Campus Marburg Marburg GermanyDepartment of Pediatric Dentistry, Dental School University of Marburg and University Medical Center Giessen and Marburg, Campus Giessen Giessen GermanyAbstract Background It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS). Aim This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application of self‐etching primers/adhesives (SEPA). Design 131 third molars were assigned to ten groups. In Hel‐P, Helioseal® was applied after phosphoric acid etching. In the other groups, SEPA were used (Dyr‐AP: Adper™ Prompt™ L‐Pop™, Dyract® Seal; Bea: BeautiSealant Primer and Paste; Hel‐Exp: Experimental primer, Helioseal®; Hel‐Cl: Clearfil™ SE Bond1, Helioseal®). Specimens were stored in distilled water at 37°C (28 days), followed by 3500 thermocycles and staining with 5% methylene blue (M) or 5% silver nitrate (S). After methylene blue staining and sectioning, microleakage was assessed light microscopically. During silver nitrate staining, specimens were dissolved by 32% HCl and remaining PFS were evaluated under a SEM. Results Tightness, percentage of penetrated area, and maximum dye penetration were best for Hel‐P and Hel‐Cl (p < 0.05). Conclusions Phosphoric acid etching of enamel and Clearfil™ SE Bond resulted in the best sealing quality. Methylene blue staining allowed the evaluation of more criteria (fissure shape, voids, sealant penetration depth) compared to silver nitrate.https://doi.org/10.1002/cre2.420etch‐and‐rinsemicroleakagepit and fissure sealingself‐etch
spellingShingle Stefanie Amend
Roland Frankenberger
Christina Boutsiouki
Vanessa Scharrelmann
Julia Winter
Norbert Krämer
Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives
Clinical and Experimental Dental Research
etch‐and‐rinse
microleakage
pit and fissure sealing
self‐etch
title Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives
title_full Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives
title_fullStr Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives
title_full_unstemmed Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives
title_short Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives
title_sort microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self etching primers adhesives
topic etch‐and‐rinse
microleakage
pit and fissure sealing
self‐etch
url https://doi.org/10.1002/cre2.420
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