New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination
The generation of energy through the transformation of polluting waste is a widely explored field and offers advances in green technologies. One of the promising technologies is Microbial Fuel Cells (MFCs). These cells can contain electroactive microorganisms that transform organic waste into electr...
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MDPI AG
2023-03-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/16/7/3047 |
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| author | Hugo Guillermo Jimenez Pacheco Abdel Alejandro Portocarrero Banda Eric Ivan Vilca Cayllahua Lilia Mary Miranda Ramos Victor Ludgardo Alvarez Tohalino Herbert Jesús Del Carpio Beltran Pavel Kewin Delgado Sarmiento |
| author_facet | Hugo Guillermo Jimenez Pacheco Abdel Alejandro Portocarrero Banda Eric Ivan Vilca Cayllahua Lilia Mary Miranda Ramos Victor Ludgardo Alvarez Tohalino Herbert Jesús Del Carpio Beltran Pavel Kewin Delgado Sarmiento |
| author_sort | Hugo Guillermo Jimenez Pacheco |
| collection | DOAJ |
| description | The generation of energy through the transformation of polluting waste is a widely explored field and offers advances in green technologies. One of the promising technologies is Microbial Fuel Cells (MFCs). These cells can contain electroactive microorganisms that transform organic waste into electricity by transferring electrons from their metabolism. In this study, a new bacterium capable of producing electricity from the waste of the poultry sector and using copper electrodes, called Av_G1, was identified and isolated. It is phylogenetically related to <i>Citrobacter freundii</i> and <i>Citrobacter Murlinae</i>. This new strain was identified molecularly, biochemically, and phylogenetically; its physiological and morphological characteristics were also studied through a Scanning Electron Microscope (SEM). Biochemical determination was performed using Simmons Citrate Agar, Lysine Iron Medium (L.I.A.), Motility/Ornithine Test, Methyl Red indicator, Enzymes: oxidase and catalase, and Gram stain test. The phylogenetic inference was deduced by bioinformatics tools (MEGA X, JalView, Clustal Omega) and the genetic databases The Ribosomal Database Project—RDP and the National Center for Biotechnology Information (NCBI). A maximum current potential of 0.645 V, a maximum current density of 168.72 ± 14.07 mA/m<sup>2</sup>, and a power density of 31.05 mW/m<sup>2</sup> were recorded. During the monitoring, the physicochemical parameters were taken: pH, Oxide Reduction Potential (ORP), Dissolved Oxygen (DO), conductivity, Total Solids (TDS), and average temperature were recorded. Therefore, the present study shows a new Gram-negative electrogenic bacterium, which can be used for electrochemical processes and applied in MFC with copper electrodes. |
| first_indexed | 2024-03-11T05:39:05Z |
| format | Article |
| id | doaj.art-aa0b341ca57e46199393bf4b76cefdbd |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-11T05:39:05Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-aa0b341ca57e46199393bf4b76cefdbd2023-11-17T16:36:41ZengMDPI AGEnergies1996-10732023-03-01167304710.3390/en16073047New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics DeterminationHugo Guillermo Jimenez Pacheco0Abdel Alejandro Portocarrero Banda1Eric Ivan Vilca Cayllahua2Lilia Mary Miranda Ramos3Victor Ludgardo Alvarez Tohalino4Herbert Jesús Del Carpio Beltran5Pavel Kewin Delgado Sarmiento6Instituto de Investigación e Innovación en Energías Renovables y Medio Ambiente, Universidad Católica de Santa María de Arequipa, Arequipa 04011, PeruInstituto de Investigación e Innovación en Energías Renovables y Medio Ambiente, Universidad Católica de Santa María de Arequipa, Arequipa 04011, PeruInstituto de Investigación e Innovación en Energías Renovables y Medio Ambiente, Universidad Católica de Santa María de Arequipa, Arequipa 04011, PeruInstituto de Investigación e Innovación en Energías Renovables y Medio Ambiente, Universidad Católica de Santa María de Arequipa, Arequipa 04011, PeruFacultad de Ingeniería de Procesos, Escuela Profesional de Ingeniería Química, Universidad Nacional de San Agustín de Arequipa, Arequipa 04011, PeruFacultad de Ingeniería y Arquitectura, Escuela Profesional de Ingeniería Industrial, Universidad Continental, Arequipa 04011, PeruFacultad de Ingeniería de Procesos, Escuela Profesional de Ingeniería Química, Universidad Nacional de San Agustín de Arequipa, Arequipa 04011, PeruThe generation of energy through the transformation of polluting waste is a widely explored field and offers advances in green technologies. One of the promising technologies is Microbial Fuel Cells (MFCs). These cells can contain electroactive microorganisms that transform organic waste into electricity by transferring electrons from their metabolism. In this study, a new bacterium capable of producing electricity from the waste of the poultry sector and using copper electrodes, called Av_G1, was identified and isolated. It is phylogenetically related to <i>Citrobacter freundii</i> and <i>Citrobacter Murlinae</i>. This new strain was identified molecularly, biochemically, and phylogenetically; its physiological and morphological characteristics were also studied through a Scanning Electron Microscope (SEM). Biochemical determination was performed using Simmons Citrate Agar, Lysine Iron Medium (L.I.A.), Motility/Ornithine Test, Methyl Red indicator, Enzymes: oxidase and catalase, and Gram stain test. The phylogenetic inference was deduced by bioinformatics tools (MEGA X, JalView, Clustal Omega) and the genetic databases The Ribosomal Database Project—RDP and the National Center for Biotechnology Information (NCBI). A maximum current potential of 0.645 V, a maximum current density of 168.72 ± 14.07 mA/m<sup>2</sup>, and a power density of 31.05 mW/m<sup>2</sup> were recorded. During the monitoring, the physicochemical parameters were taken: pH, Oxide Reduction Potential (ORP), Dissolved Oxygen (DO), conductivity, Total Solids (TDS), and average temperature were recorded. Therefore, the present study shows a new Gram-negative electrogenic bacterium, which can be used for electrochemical processes and applied in MFC with copper electrodes.https://www.mdpi.com/1996-1073/16/7/3047electrogenicphylogeneticcitrobacterenterobacterexoelectrogenicelectrotrophic |
| spellingShingle | Hugo Guillermo Jimenez Pacheco Abdel Alejandro Portocarrero Banda Eric Ivan Vilca Cayllahua Lilia Mary Miranda Ramos Victor Ludgardo Alvarez Tohalino Herbert Jesús Del Carpio Beltran Pavel Kewin Delgado Sarmiento New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination Energies electrogenic phylogenetic citrobacter enterobacter exoelectrogenic electrotrophic |
| title | New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination |
| title_full | New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination |
| title_fullStr | New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination |
| title_full_unstemmed | New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination |
| title_short | New Electrogenic Microorganism <i>Citrobacter</i> sp. Isolated from Microbial Fuel Cell and Bacterial Characteristics Determination |
| title_sort | new electrogenic microorganism i citrobacter i sp isolated from microbial fuel cell and bacterial characteristics determination |
| topic | electrogenic phylogenetic citrobacter enterobacter exoelectrogenic electrotrophic |
| url | https://www.mdpi.com/1996-1073/16/7/3047 |
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