Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration
Diabetic ulcers remain a significant challenge in wound care due to loss of epithelial cell migration. Aligned nanofibrous scaffolds mimicking the skin's extracellular matrix (ECM) are promising candidates for diabetic wound healing. In this study, a composition of poly(ε-caprolactone), gelatin...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127524000662 |
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| author | Erfan Rezvani Ghomi Venkatesh Mayandi Vijila Chellappan Nileshkumar Dubey Kottaiswamy Amuthavalli Rasoul Esmaeely Neisiany Veluchamy Amutha Barathi Navin Kumar Verma Rajamani Lakshminarayanan Seeram Ramakrishna |
| author_facet | Erfan Rezvani Ghomi Venkatesh Mayandi Vijila Chellappan Nileshkumar Dubey Kottaiswamy Amuthavalli Rasoul Esmaeely Neisiany Veluchamy Amutha Barathi Navin Kumar Verma Rajamani Lakshminarayanan Seeram Ramakrishna |
| author_sort | Erfan Rezvani Ghomi |
| collection | DOAJ |
| description | Diabetic ulcers remain a significant challenge in wound care due to loss of epithelial cell migration. Aligned nanofibrous scaffolds mimicking the skin's extracellular matrix (ECM) are promising candidates for diabetic wound healing. In this study, a composition of poly(ε-caprolactone), gelatin, and dopamine-containing varying amounts of ε-polylysine (ε-PL) was electrospun to prepare aligned nanofiber wound dressings (ANFDs). We then investigated the morphological, physicochemical, mechanical, and biological properties of fabricated ANDFs. The presence of ε-PL confers bactericidal properties while promoting epithelial and fibroblast adhesion, proliferation, and migration, confirming its cell selectivity. The clinical importance of the ANFDs was then demonstrated in a mice model of full-thickness diabetic wounds. The results confirm that ANFD treatment resulted in a higher rate of wound closure in the linear range of wound closure than wounds treated with silver dressings. Taken together, these results suggest the potential of antimicrobial ANFDs for the treatment of diabetic wounds. |
| first_indexed | 2024-03-07T23:25:00Z |
| format | Article |
| id | doaj.art-aa0be1331665464b90316f18fe6bc1ed |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-07T23:25:00Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-aa0be1331665464b90316f18fe6bc1ed2024-02-21T05:24:06ZengElsevierMaterials & Design0264-12752024-02-01238112694Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regenerationErfan Rezvani Ghomi0Venkatesh Mayandi1Vijila Chellappan2Nileshkumar Dubey3Kottaiswamy Amuthavalli4Rasoul Esmaeely Neisiany5Veluchamy Amutha Barathi6Navin Kumar Verma7Rajamani Lakshminarayanan8Seeram Ramakrishna9Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore 117581, Singapore; Ocular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore; Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore 308232, Singapore; A.J. Drexel Nanomaterials Institute, Drexel University, Philadelphia, PA 19104, United StatesOcular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, SingaporeInstitute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore; Corresponding authors at: Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), #08-03, Singapore, 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore (V. Chellappan), Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore, 308232, Singapore (N.K. Verma), Ocular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore (R. Lakshminarayanan), Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore, 117581, Singapore (S. Ramakrishna).Faculty of Dentistry, National University of Singapore, Singapore 119085, Singapore; ORCHIDS: Oral Care Health Innovations and Designs Singapore, National University of Singapore, Singapore, Singapore 119085, SingaporeLee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore 308232, SingaporeDepartment of Polymer Engineering, Hakim Sabzevari University, Sabzevar 9617976487, Iran; Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 44-100, Gliwice, PolandOcular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, SingaporeOcular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore 308232, Singapore; National Skin Centre, 1 Mandalay Road, Singapore 308205, Singapore; Skin Research Institute of Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore 308232, Singapore; Corresponding authors at: Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), #08-03, Singapore, 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore (V. Chellappan), Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore, 308232, Singapore (N.K. Verma), Ocular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore (R. Lakshminarayanan), Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore, 117581, Singapore (S. Ramakrishna).Ocular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore; Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Medical School, Singapore 169857, Singapore; Department of Pharmacy, National University of Singapore, Singapore 117559, Singapore; Corresponding authors at: Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), #08-03, Singapore, 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore (V. Chellappan), Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore, 308232, Singapore (N.K. Verma), Ocular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore (R. Lakshminarayanan), Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore, 117581, Singapore (S. Ramakrishna).Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore 117581, Singapore; Corresponding authors at: Institute of Materials Research and Engineering (IMRE), Agency for Science Technology and Research (A*STAR), #08-03, Singapore, 2 Fusionopolis Way, Innovis #08-03, Singapore 138634, Singapore (V. Chellappan), Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Singapore, 308232, Singapore (N.K. Verma), Ocular Infections and Antimicrobials Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore (R. Lakshminarayanan), Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore, 117581, Singapore (S. Ramakrishna).Diabetic ulcers remain a significant challenge in wound care due to loss of epithelial cell migration. Aligned nanofibrous scaffolds mimicking the skin's extracellular matrix (ECM) are promising candidates for diabetic wound healing. In this study, a composition of poly(ε-caprolactone), gelatin, and dopamine-containing varying amounts of ε-polylysine (ε-PL) was electrospun to prepare aligned nanofiber wound dressings (ANFDs). We then investigated the morphological, physicochemical, mechanical, and biological properties of fabricated ANDFs. The presence of ε-PL confers bactericidal properties while promoting epithelial and fibroblast adhesion, proliferation, and migration, confirming its cell selectivity. The clinical importance of the ANFDs was then demonstrated in a mice model of full-thickness diabetic wounds. The results confirm that ANFD treatment resulted in a higher rate of wound closure in the linear range of wound closure than wounds treated with silver dressings. Taken together, these results suggest the potential of antimicrobial ANFDs for the treatment of diabetic wounds.http://www.sciencedirect.com/science/article/pii/S0264127524000662ElectrospinningWound healingAligned nanofiberBiomaterialsBacterial infections |
| spellingShingle | Erfan Rezvani Ghomi Venkatesh Mayandi Vijila Chellappan Nileshkumar Dubey Kottaiswamy Amuthavalli Rasoul Esmaeely Neisiany Veluchamy Amutha Barathi Navin Kumar Verma Rajamani Lakshminarayanan Seeram Ramakrishna Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration Materials & Design Electrospinning Wound healing Aligned nanofiber Biomaterials Bacterial infections |
| title | Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration |
| title_full | Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration |
| title_fullStr | Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration |
| title_full_unstemmed | Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration |
| title_short | Biomimetic aligned nanofibrous dressings containing cell-selective polymer enhance diabetic wound regeneration |
| title_sort | biomimetic aligned nanofibrous dressings containing cell selective polymer enhance diabetic wound regeneration |
| topic | Electrospinning Wound healing Aligned nanofiber Biomaterials Bacterial infections |
| url | http://www.sciencedirect.com/science/article/pii/S0264127524000662 |
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